{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:16:37Z","timestamp":1791206197164,"version":"4.1.0"},"reference-count":49,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":6595,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,10]]},"abstract":"<jats:p>Cytokines produced in the female reproductive tract after mating may enhance reproductive success. The present study investigated the distribution of granulocyte\u2013macrophage colony stimulating factor (GM-CSF) and interleukin (IL)-8 in tissues and luminal secretions from different sites in the reproductive tract of the ewe following oestrus and after natural mating. Fifteen ewes were mated with a ram for 1 h and their reproductive tracts collected 3, 6, 18, 24 or 48 h later. Another 15 ewes were used as oestrous controls. Luminal secretions and tissue samples were collected from seven sites in each reproductive tract. Secretions were analysed by enzyme-linked immunosorbent assay and tissues were stained immunohistochemically using anti-sheep GM-CSF and anti-sheep IL-8 antibodies. Both cytokines were found in luminal and glandular endometrial epithelium and, to a lesser extent, in cervical epithelium; neither was found in the vaginal epithelium. Twice as many (P &amp;lt; 0.05) luminal samples from mated ewes than non-mated ewes were positive for GM-CSF. The vaginal lumen contained significantly higher (P &amp;lt; 0.01) concentrations of IL-8 compared with other sites, irrespective of mating status. Significant differences (P &amp;lt; 0.05) were found in staining intensity of GM-CSF and IL-8 from different sites. Production of GM-CSF and IL-8 by reproductive tissues is likely to contribute to leucocyte infiltration into the ovine reproductive tract.<\/jats:p>","DOI":"10.1071\/rd06137","type":"journal-article","created":{"date-parts":[[2007,5,10]],"date-time":"2007-05-10T17:52:04Z","timestamp":1178819524000},"page":"585-593","source":"Crossref","is-referenced-by-count":9,"title":["Granulocyte\u2013macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating"],"prefix":"10.1071","volume":"19","author":[{"given":"Jennifer L.","family":"Scott","sequence":"first","affiliation":[{"name":"ASchool of Veterinary and Biomedical Sciences, James Cook University, Solander Drive, Douglas, QLD 4811, Australia."},{"name":"BCorresponding author. Email: jennifer.scott1@jcu.edu.au"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natkunam","family":"Ketheesan","sequence":"additional","affiliation":[{"name":"ASchool of Veterinary and Biomedical Sciences, James Cook University, Solander Drive, Douglas, QLD 4811, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip M.","family":"Summers","sequence":"additional","affiliation":[{"name":"ASchool of Veterinary and Biomedical Sciences, James Cook University, Solander Drive, Douglas, QLD 4811, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2007,5,7]]},"reference":[{"key":"2025102818353156500_R1"},{"key":"2025102818353156500_R2"},{"key":"2025102818353156500_R3"},{"key":"2025102818353156500_R4"},{"key":"2025102818353156500_R5"},{"key":"2025102818353156500_R6"},{"key":"2025102818353156500_R7"},{"key":"2025102818353156500_R8"},{"key":"2025102818353156500_R9"},{"key":"2025102818353156500_R10"},{"key":"2025102818353156500_R11"},{"key":"2025102818353156500_R12"},{"key":"2025102818353156500_R13"},{"key":"2025102818353156500_R14"},{"key":"2025102818353156500_R15"},{"key":"2025102818353156500_R16"},{"key":"2025102818353156500_R17"},{"key":"2025102818353156500_R18"},{"key":"2025102818353156500_R19"},{"key":"2025102818353156500_R20"},{"key":"2025102818353156500_R21"},{"key":"2025102818353156500_R22"},{"key":"2025102818353156500_R23"},{"key":"2025102818353156500_R24"},{"key":"2025102818353156500_R25"},{"key":"2025102818353156500_R26"},{"key":"2025102818353156500_R27"},{"key":"2025102818353156500_R28"},{"key":"2025102818353156500_R29"},{"key":"2025102818353156500_R30"},{"key":"2025102818353156500_R31"},{"key":"2025102818353156500_R32"},{"key":"2025102818353156500_R33"},{"key":"2025102818353156500_R34"},{"key":"2025102818353156500_R35"},{"key":"2025102818353156500_R36"},{"key":"2025102818353156500_R37"},{"key":"2025102818353156500_R38"},{"key":"2025102818353156500_R39"},{"key":"2025102818353156500_R40"},{"key":"2025102818353156500_R41"},{"key":"2025102818353156500_R42"},{"key":"2025102818353156500_R43"},{"key":"2025102818353156500_R44"},{"key":"2025102818353156500_R45"},{"key":"2025102818353156500_R46"},{"key":"2025102818353156500_R47"},{"key":"2025102818353156500_R48"},{"key":"2025102818353156500_R49"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/19\/4\/585\/301046\/rd06137.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/19\/4\/585\/301046\/rd06137.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:35:40Z","timestamp":1761690940000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/19\/4\/585\/48481\/Granulocyte-macrophage-colony-stimulating-factor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,7]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,5,10]]}},"URL":"https:\/\/doi.org\/10.1071\/rd06137","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,7]]}}}