{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:41:24Z","timestamp":1767854484484,"version":"3.49.0"},"reference-count":62,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":5884,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,4,17]]},"abstract":"<jats:p>In the present study, the viability, intracellular pH (pHi), cAMP ([cAMP]i), calcium concentration and protein phosphotyrosine content were evaluated in relation to the acrosomal and capacitation status of freshly ejaculated bull spermatozoa. These parameters were evaluated before and after incubation with the capacitation inducer heparin, the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), the phosphotyrosyl-protein phosphatase inhibitors phenylarsine oxide (PAO) and sodium orthovanadate, and hydrogen peroxide. The results obtained were integrated to address the physiological interactions between the different signalling events affecting sperm capacitation and acrosome reaction. As expected, heparin promoted the expression of the \u2018B\u2019 pattern of chlortetracycline binding, increased pHi, [cAMP]i and the phosphotyrosine content of sperm proteins. The effects of heparin were enhanced by IBMX. Both PAO and sodium orthovanadate stimulated protein phosphotyrosine content and acrosomal exocytosis, although only PAO affected pH, Ca2+ and cAMP levels. Intracellular pH was increased while both Ca2+ and [cAMP]i were decreased. Physiological concentrations of H2O2 increased [cAMP]i and promoted acrosomal exocytosis. A significant positive correlation was found between sperm capacitation, protein phosphotyrosine content and stored Ca2+ concentration, whereas the acrosome reaction was correlated with pHi and Ca2+ concentration. This study presents the first global analysis of the major elements individually described during sperm capacitation and acrosome reaction signalling pathways, supported by statistical correlations.<\/jats:p>","DOI":"10.1071\/rd07169","type":"journal-article","created":{"date-parts":[[2009,4,16]],"date-time":"2009-04-16T23:21:21Z","timestamp":1239924081000},"page":"511-524","source":"Crossref","is-referenced-by-count":28,"title":["Modulation of bovine sperm signalling pathways: correlation between intracellular parameters and sperm capacitation and acrosome exocytosis"],"prefix":"10.1071","volume":"21","author":[{"given":"Hanae","family":"Pons-Rejraji","sequence":"first","affiliation":[{"name":"AD\u00e9partement d\u2019Obst\u00e9trique-Gyn\u00e9cologie, Centre de Recherche en Biologie de la Reproduction, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 4G2, Canada."},{"name":"CUnit\u00e9 d\u2019Ontogenie et Reproduction, Centre de recherche du CHUQ-CHUL, 2705 boul Laurier, Ste-Foy, QC G1V 4G2, Canada."},{"name":"DPresent address: Laboratoire Biologie du D\u00e9veloppement et de la Reproduction, UE 975, Facult\u00e9 de M\u00e9decine-Clermont 1, 28 place Henri Dunant, 63001 Clermont-Ferrand Cedex, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice L.","family":"Bailey","sequence":"additional","affiliation":[{"name":"BD\u00e9partement des Sciences Animales, Centre de Recherche en Biologie de la Reproduction, Universit\u00e9 Laval, Qu\u00e9bec, QC G1K 7P4, Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Leclerc","sequence":"additional","affiliation":[{"name":"AD\u00e9partement d\u2019Obst\u00e9trique-Gyn\u00e9cologie, Centre de Recherche en Biologie de la Reproduction, Universit\u00e9 Laval, Qu\u00e9bec, QC G1V 4G2, Canada."},{"name":"CUnit\u00e9 d\u2019Ontogenie et Reproduction, Centre de recherche du CHUQ-CHUL, 2705 boul Laurier, Ste-Foy, QC G1V 4G2, Canada."},{"name":"ECorresponding author. Email: pierre.leclerc@crchul.ulaval.ca"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2009,4,17]]},"reference":[{"key":"2025102818385287700_R1"},{"key":"2025102818385287700_R2"},{"key":"2025102818385287700_R3"},{"key":"2025102818385287700_R4"},{"key":"2025102818385287700_R5"},{"key":"2025102818385287700_R6"},{"key":"2025102818385287700_R7"},{"key":"2025102818385287700_R8"},{"key":"2025102818385287700_R9"},{"key":"2025102818385287700_R10"},{"key":"2025102818385287700_R11"},{"key":"2025102818385287700_R12"},{"key":"2025102818385287700_R13"},{"key":"2025102818385287700_R14"},{"key":"2025102818385287700_R15"},{"key":"2025102818385287700_R16"},{"key":"2025102818385287700_R17"},{"key":"2025102818385287700_R18"},{"key":"2025102818385287700_R19"},{"key":"2025102818385287700_R20"},{"key":"2025102818385287700_R21"},{"key":"2025102818385287700_R22"},{"key":"2025102818385287700_R23"},{"key":"2025102818385287700_R24"},{"key":"2025102818385287700_R25"},{"key":"2025102818385287700_R26"},{"key":"2025102818385287700_R27"},{"key":"2025102818385287700_R28"},{"key":"2025102818385287700_R29"},{"key":"2025102818385287700_R30"},{"key":"2025102818385287700_R31"},{"key":"2025102818385287700_R32"},{"key":"2025102818385287700_R33"},{"key":"2025102818385287700_R34"},{"key":"2025102818385287700_R35"},{"key":"2025102818385287700_R36"},{"key":"2025102818385287700_R37"},{"key":"2025102818385287700_R38"},{"key":"2025102818385287700_R39"},{"key":"2025102818385287700_R40"},{"key":"2025102818385287700_R41"},{"key":"2025102818385287700_R42"},{"key":"2025102818385287700_R43"},{"key":"2025102818385287700_R44"},{"key":"2025102818385287700_R45"},{"key":"2025102818385287700_R46"},{"key":"2025102818385287700_R47"},{"key":"2025102818385287700_R48"},{"key":"2025102818385287700_R49"},{"key":"2025102818385287700_R50"},{"key":"2025102818385287700_R51"},{"key":"2025102818385287700_R52"},{"key":"2025102818385287700_R53"},{"key":"2025102818385287700_R54"},{"key":"2025102818385287700_R55"},{"key":"2025102818385287700_R56"},{"key":"2025102818385287700_R57"},{"key":"2025102818385287700_R58"},{"key":"2025102818385287700_R59"},{"key":"2025102818385287700_R60"},{"key":"2025102818385287700_R61"},{"key":"2025102818385287700_R62"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/21\/4\/511\/301661\/rd07169.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/21\/4\/511\/301661\/rd07169.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:38:57Z","timestamp":1761691137000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/21\/4\/511\/48554\/Modulation-of-bovine-sperm-signalling-pathways"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4,17]]},"references-count":62,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4,17]]}},"URL":"https:\/\/doi.org\/10.1071\/rd07169","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,4,17]]}}}