{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:17:44Z","timestamp":1767842264632,"version":"3.49.0"},"reference-count":50,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":5928,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,4]]},"abstract":"<jats:p>The male reproductive tract of ungulates contains two protein families bearing tandemly arranged fibronectin II (Fn2) modules; one (small Fn2 proteins) bears two modules (e.g. BSP\u2013A1\/2), the other (long Fn2 proteins) bears four (e.g. epididymal sperm-binding protein 1 (ELSPBP1)). While it is well known that small Fn2 proteins are present in bull semen, nothing is known about long Fn2 proteins. In the present study, the presence of ELSPBP1 proteins in the bull epididymis and their association with maturing spermatozoa were investigated using a specific antibody against canine ELSPBP1. Analysis of western blots showed ELSPBP1 to be present in the caput, corpus and cauda regions of the epididymis. The protein, which bound phosphorylcholine (PC) strongly, appeared to associate with the spermatozoa during maturation because it was absent from caput spermatozoa but present on cauda spermatozoa. Immunocytochemistry of cauda spermatozoa showed the protein to be bound to the post-acrosomal and midpiece regions. ELSPBP1 could not be detected on freshly ejaculated spermatozoa but was revealed after a capacitating treatment. Our previous studies have shown differences between bovine caput and cauda spermatozoa in terms of their ability to control cell volume. Because of the close homology of BSP\u2013A1\/2 PC binding regions with Fn2 regions in ELSPBP1, BSP\u2013A1\/2 was used as a model to investigate the effect of a PC-binding Fn2 protein on cell volume control. While the protein had no effect on cauda spermatozoa, it caused caput spermatozoa to swell more in response to hypotonic stress, similarly to untreated cauda spermatozoa.<\/jats:p>","DOI":"10.1071\/rd08209","type":"journal-article","created":{"date-parts":[[2009,3,3]],"date-time":"2009-03-03T22:20:21Z","timestamp":1236118821000},"page":"479-488","source":"Crossref","is-referenced-by-count":25,"title":["Fibronectin type II-module proteins in the bovine genital tract and their putative role in cell volume control during sperm maturation"],"prefix":"10.1071","volume":"21","author":[{"given":"Evrim","family":"Sahin","sequence":"first","affiliation":[{"name":"AClinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."},{"name":"BClinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna M.","family":"Petrunkina","sequence":"additional","affiliation":[{"name":"CClinic for Horses, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."},{"name":"FCambridge Institute for Medical Research, University of Cambridge, Welcome Trust\/MRC Building, Hills Road, Cambridge CB2 0XY, UK."},{"name":"GCorresponding authors. Email: edda.toepfer-petersen@tiho-hannover.de"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahnaz","family":"Ekhlasi-Hundrieser","sequence":"additional","affiliation":[{"name":"BClinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiane","family":"Hettel","sequence":"additional","affiliation":[{"name":"BClinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmar","family":"Waberski","sequence":"additional","affiliation":[{"name":"AClinic for Pigs and Small Ruminants, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin A. P.","family":"Harrison","sequence":"additional","affiliation":[{"name":"D11 London Road, Great Shelford, Cambridge CB22 5DB, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edda","family":"T\u00f6pfer-Petersen","sequence":"additional","affiliation":[{"name":"EInstitute for Reproductive Biology, University of Veterinary Medicine Hannover, Foundation, Unit of Reproductive Medicine of the Clinics, B\u00fcnteweg 15, Hannover 30559, Germany."},{"name":"GCorresponding authors. Email: edda.toepfer-petersen@tiho-hannover.de"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2009,3,4]]},"reference":[{"key":"2025102818432082700_R1"},{"key":"2025102818432082700_R2"},{"key":"2025102818432082700_R3"},{"key":"2025102818432082700_R4"},{"key":"2025102818432082700_R5"},{"key":"2025102818432082700_R6"},{"key":"2025102818432082700_R7"},{"key":"2025102818432082700_R8"},{"key":"2025102818432082700_R9"},{"key":"2025102818432082700_R10"},{"key":"2025102818432082700_R11"},{"key":"2025102818432082700_R12"},{"key":"2025102818432082700_R13"},{"key":"2025102818432082700_R14"},{"key":"2025102818432082700_R15"},{"key":"2025102818432082700_R16"},{"key":"2025102818432082700_R17"},{"key":"2025102818432082700_R18"},{"key":"2025102818432082700_R19"},{"key":"2025102818432082700_R20"},{"key":"2025102818432082700_R21"},{"key":"2025102818432082700_R22"},{"key":"2025102818432082700_R23"},{"key":"2025102818432082700_R24"},{"key":"2025102818432082700_R25"},{"key":"2025102818432082700_R26"},{"key":"2025102818432082700_R27"},{"key":"2025102818432082700_R28"},{"key":"2025102818432082700_R29"},{"key":"2025102818432082700_R30"},{"key":"2025102818432082700_R31"},{"key":"2025102818432082700_R32"},{"key":"2025102818432082700_R33"},{"key":"2025102818432082700_R34"},{"key":"2025102818432082700_R35"},{"key":"2025102818432082700_R36"},{"key":"2025102818432082700_R37"},{"key":"2025102818432082700_R38"},{"key":"2025102818432082700_R39"},{"key":"2025102818432082700_R40"},{"key":"2025102818432082700_R41"},{"key":"2025102818432082700_R42"},{"key":"2025102818432082700_R43"},{"key":"2025102818432082700_R44"},{"key":"2025102818432082700_R45"},{"key":"2025102818432082700_R46"},{"key":"2025102818432082700_R47"},{"key":"2025102818432082700_R48"},{"key":"2025102818432082700_R49"},{"key":"2025102818432082700_R50"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/21\/3\/479\/302541\/rd08209.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/21\/3\/479\/302541\/rd08209.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:43:30Z","timestamp":1761691410000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/21\/3\/479\/48650\/Fibronectin-type-II-module-proteins-in-the-bovine"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,4]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3,4]]}},"URL":"https:\/\/doi.org\/10.1071\/rd08209","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,4]]}}}