{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T22:45:33Z","timestamp":1789685133359,"version":"4.0.1"},"reference-count":42,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":5529,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,29]]},"abstract":"<jats:p>Insulin-like 3 (INSL3) plays a prominent role in male development and is supposed to induce the growth of the gubernaculum testis (g.t.), thus being directly involved in testicular descent in humans and rodents. This happens through activation of the RXFP2 receptor (GREAT or LGR8). The INSL3\u2013RXFP2 complex is reputed to play an additional paracrine role in the testis, possibly acting as part of an autocrine feedback loop. The present work provides evidence of the immunolocalisation of INSL3 in the Leydig cells of canine fetuses and of the expression of RXFP2 receptor in different tissues of the g.t. of the same specimens. RXFP2 was localised at the cell membrane of g.t. muscle and connective cells, as well as in the epithelial cells of the developing excurrent ducts. Notably, RXFP2 immunoreactivity of the g.t. was limited to fetuses at ~35\u201345 days of gestation, which is also the fetal period when the endocrine compartment of the dog testis is active endocrinologically, as confirmed by the anti-P450c17 and anti-INSL3 immunoreactivities of the fetal Leydig cells, and by anti-M\u00fcllerian hormone immunoreactivity of the Sertoli cells. The same immunoreactivities were also evaluated in the testes of cryptorchid dogs of different ages. RXFP2 immunoreactivity was absent from genital tracts of cryptorchid testes and g.t. remnants.<\/jats:p>","DOI":"10.1071\/rd09260","type":"journal-article","created":{"date-parts":[[2010,4,28]],"date-time":"2010-04-28T21:25:20Z","timestamp":1272489920000},"page":"751-760","source":"Crossref","is-referenced-by-count":18,"title":["An insight into testis and gubernaculum dynamics of INSL3\u2013RXFP2 signalling during testicular descent in the dog"],"prefix":"10.1071","volume":"22","author":[{"given":"S.","family":"Arrighi","sequence":"first","affiliation":[{"name":"ADepartment of Veterinary Sciences and Technologies for Food Safety, Laboratory of Anatomy, Universit\u00e0 degli Studi di Milano, Italy."},{"name":"CCorresponding author. Email: silvana.arrighi@unimi.it"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bosi","sequence":"additional","affiliation":[{"name":"ADepartment of Veterinary Sciences and Technologies for Food Safety, Laboratory of Anatomy, Universit\u00e0 degli Studi di Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Groppetti","sequence":"additional","affiliation":[{"name":"BDepartment of Veterinary Clinical Science, Reproduction Unit, Faculty of Veterinary Medicine, Universit\u00e0 degli Studi di Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Aralla","sequence":"additional","affiliation":[{"name":"ADepartment of Veterinary Sciences and Technologies for Food Safety, Laboratory of Anatomy, Universit\u00e0 degli Studi di Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Cremonesi","sequence":"additional","affiliation":[{"name":"BDepartment of Veterinary Clinical Science, Reproduction Unit, Faculty of Veterinary Medicine, Universit\u00e0 degli Studi di Milano, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2010,4,7]]},"reference":[{"key":"2025102819000074300_R1"},{"key":"2025102819000074300_R2"},{"key":"2025102819000074300_R3"},{"key":"2025102819000074300_R4"},{"key":"2025102819000074300_R5"},{"key":"2025102819000074300_R6"},{"key":"2025102819000074300_R7"},{"key":"2025102819000074300_R8"},{"key":"2025102819000074300_R9"},{"key":"2025102819000074300_R10"},{"key":"2025102819000074300_R11"},{"key":"2025102819000074300_R12"},{"key":"2025102819000074300_R13"},{"key":"2025102819000074300_R14"},{"key":"2025102819000074300_R15"},{"key":"2025102819000074300_R16"},{"key":"2025102819000074300_R17"},{"key":"2025102819000074300_R18"},{"key":"2025102819000074300_R19"},{"key":"2025102819000074300_R20"},{"key":"2025102819000074300_R21"},{"key":"2025102819000074300_R22"},{"key":"2025102819000074300_R23"},{"key":"2025102819000074300_R24"},{"key":"2025102819000074300_R25"},{"key":"2025102819000074300_R26"},{"key":"2025102819000074300_R27"},{"key":"2025102819000074300_R28"},{"key":"2025102819000074300_R29"},{"key":"2025102819000074300_R30"},{"key":"2025102819000074300_R31"},{"key":"2025102819000074300_R32"},{"key":"2025102819000074300_R33"},{"key":"2025102819000074300_R34"},{"key":"2025102819000074300_R35"},{"key":"2025102819000074300_R36"},{"key":"2025102819000074300_R37"},{"key":"2025102819000074300_R38"},{"key":"2025102819000074300_R39"},{"key":"2025102819000074300_R40"},{"key":"2025102819000074300_R41"},{"key":"2025102819000074300_R42"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/22\/5\/751\/304351\/rd09260.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/22\/5\/751\/304351\/rd09260.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:00:07Z","timestamp":1761692407000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/22\/5\/751\/49011\/An-insight-into-testis-and-gubernaculum-dynamics"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4,7]]},"references-count":42,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,4,29]]}},"URL":"https:\/\/doi.org\/10.1071\/rd09260","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4,7]]}}}