{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:08:38Z","timestamp":1791119318958,"version":"4.1.0"},"reference-count":48,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":5030,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,8,19]]},"abstract":"<jats:p>The present study aimed to develop a set of sensitive assays to evaluate the presence of different isoforms, the activity degree, and the immunolocalisation of proacrosin\u2013acrosin in sexually mature boars. The goal was to determine the proacrosin\u2013acrosin status of boar spermatozoa throughout epididymal maturation, during ejaculation and after in vitro capacitation. In epididymal samples, proacrosin expression was high in all regions studied. In contrast, a- and \u00df-acrosin expression was low in the caput region, and increased progressively during maturation and in vitro capacitation. In in vitro capacitated samples, the acrosin activity was 2.25 times higher than in the ejaculated samples and immunolocalisation analyses showed redistribution of proacrosin\u2013acrosin at the apical ridge of the head. This study provides relevant data about the expression, localisation and activity of the proacrosin\u2013acrosin system in healthy adult boars that can be used as a base to analyse changes in the proacrosin\u2013acrosin system under pathological conditions.<\/jats:p>","DOI":"10.1071\/rd10345","type":"journal-article","created":{"date-parts":[[2011,8,25]],"date-time":"2011-08-25T21:13:06Z","timestamp":1314306786000},"page":"837-845","source":"Crossref","is-referenced-by-count":31,"title":["Study of the proacrosin\u2013acrosin system in epididymal, ejaculated and in vitro capacitated boar spermatozoa"],"prefix":"10.1071","volume":"23","author":[{"given":"Marta","family":"Puigmul\u00e9","sequence":"first","affiliation":[{"name":"ABiotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, Campus of Montilivi, s\/n, 17071 Girona, Spain."},{"name":"CCorresponding author. 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