{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:40:43Z","timestamp":1791168043048,"version":"4.1.0"},"reference-count":38,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4941,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,23]]},"abstract":"<jats:p>Poor fertility rates are often observed when fresh ram semen stored in conventional extenders is used for cervical artificial insemination (AI). Heat-shock 70-kDa protein 8 (HSPA8), found within the oviduct, prolongs boar, ram and bull sperm survival at body temperatures in vitro. Here, we aimed to determine whether supplementing extenders (INRA-96 and RSD-1) with HSPA8 (4 \u00b5g mL\u20131) would improve their performance in maintaining freshly collected ram sperm viability and sperm nuclear DNA integrity during storage over 48 h at 17\u00b0C. Sperm function was assessed at 1, 6, 24 and 48 h and this experiment was repeated using 25 \u00d7 106 and 800 \u00d7 106 spermatozoa mL\u20131. INRA96 supplemented with HSPA8 maintained sperm viability significantly better than INRA96 alone at both sperm concentrations. However, sperm nuclear DNA fragmentation (DF) increased significantly during storage using the higher sperm concentration, irrespective of the extender and the protein treatment used. Increasing levels of sperm nuclear DF over time could explain why poor fertility rates are often observed following cervical AI using stored ram semen. However, further research is required to ascertain whether supplementing the commercially available INRA96 extender with HSPA8 will improve fertility rates following cervical AI using stored ram semen.<\/jats:p>","DOI":"10.1071\/rd11173","type":"journal-article","created":{"date-parts":[[2011,11,16]],"date-time":"2011-11-16T10:02:13Z","timestamp":1321437733000},"page":"543-549","source":"Crossref","is-referenced-by-count":33,"title":["The oviducal protein, heat-shock 70-kDa protein 8, improves the long-term survival of ram spermatozoa during storage at 17\u00b0C in a commercial extender"],"prefix":"10.1071","volume":"24","author":[{"given":"R. E.","family":"Lloyd","sequence":"first","affiliation":[{"name":"AInstitute of Zoology, Regent\u2019s Park, London NW1 4RY, UK."},{"name":"DPresent address: Nuffield Building, Institute of Zoology, Regent\u2019s Park, London NW1 4RY, UK."},{"name":"ECorresponding author. Email: rhiannon.lloyd@ioz.ac.uk"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fazeli","sequence":"additional","affiliation":[{"name":"BAcademic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Level 4, The Jessop Wing, Tree Root Walk, Sheffield S10 2SF, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. F.","family":"Watson","sequence":"additional","affiliation":[{"name":"CRoyal Veterinary College, Royal College Street, London, NW1 0TU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. V.","family":"Holt","sequence":"additional","affiliation":[{"name":"AInstitute of Zoology, Regent\u2019s Park, London NW1 4RY, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2011,11,16]]},"reference":[{"issue":"Suppl. 2","key":"2025102819245123900_R1","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1111\/J.1439-0531.2006.00767.X","article-title":"Improvement strategies in ovine artificial insemination.","volume":"41","author":"Anel","year":"2006","journal-title":"Reprod. Domest. Anim."},{"key":"2025102819245123900_R2","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1083\/JCB.97.1.209","article-title":"Evidence for tubulin-binding sites on cellular membranes: plasma membranes, mitochondrial membranes and secretory granule membranes.","volume":"97","author":"Bernier-Valentin","year":"1983","journal-title":"J. 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