{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:13:40Z","timestamp":1791166420014,"version":"4.1.0"},"reference-count":60,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4827,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,28]]},"abstract":"<jats:p>Polycystic ovary syndrome (PCOS) is one of the most common causes of anovulatory infertility, affecting 5\u201310% of females during their reproductive life. Currently the pathology of PCOS is largely unknown. To identify the differential protein expression in follicular fluids from PCOS and normal subjects during controlled ovarian hyperstimulation, we performed an initial proteomic study including two-dimensional gel electrophoresis (2DE) analysis and mass spectroscopy, and confirmed results by western blot. Thirty-two protein spots were shown to be significantly differentially expressed between PCOS and normal follicular fluids, of which 20 unique proteins were identified to be associated with cellular metabolism and physiological processes; 13 of these proteins were upregulated while seven were downregulated in PCOS follicular fluids. Western blotting analyses confirmed the differential expressions for three randomly selected proteins, i.e. upregulated a1-antitrypsin, apolipoprotein A-I and transferrin in follicular fluid from PCOS patients than normal controls. Furthermore, semiquantitative reverse transcription-polymerase chain reaction (RT\u2013PCR) analyses revealed that mRNA levels of serine palmitoyltransferase 2, serine\/threonine-protein kinase male germ cell-associated kinase (MAK) and DNA damage-regulated autophagy modulator protein 2 decreased significantly in granulosa cells of PCOS patients compared with normal samples. These results increase our understanding of PCOS and the identified genes may serve as candidate biomarkers to develop diagnostic and therapeutic tools.<\/jats:p>","DOI":"10.1071\/rd11201","type":"journal-article","created":{"date-parts":[[2012,3,8]],"date-time":"2012-03-08T23:18:02Z","timestamp":1331248682000},"page":"893-904","source":"Crossref","is-referenced-by-count":46,"title":["Different protein expression patterns associated with polycystic ovary syndrome in human follicular fluid during controlled ovarian hyperstimulation"],"prefix":"10.1071","volume":"24","author":[{"given":"Guo","family":"Dai","sequence":"first","affiliation":[{"name":"AInstitute of Reproductive and Stem Cell Engineering, Central South University, Xiangya Road 84, Changsha 410078, People\u2019s Republic of China."},{"name":"DCollege of Life Science, Hunan Normal University, Lushan Road 36, Changsha 410081, People\u2019s Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangxiu","family":"Lu","sequence":"additional","affiliation":[{"name":"AInstitute of Reproductive and Stem Cell Engineering, Central South University, Xiangya Road 84, Changsha 410078, People\u2019s Republic of China."},{"name":"BReproductive and Genetic Hospital of CITIC-XIANGYA, Xiangya Road 84, Changsha 410078, People\u2019s Republic of China."},{"name":"CNational Engineering and Research Center of Human Stem Cell, Xiangya Road 84, Changsha 410078, People\u2019s Republic of China."},{"name":"ECorresponding author. 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