{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T05:37:33Z","timestamp":1779946653438,"version":"3.53.1"},"reference-count":44,"publisher":"CSIRO Publishing","issue":"8","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4779,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,10,5]]},"abstract":"<jats:p>Artificial insemination (AI) is a potentially useful tool for breeding captive elephants because it facilitates efforts to minimise inbreeding. However, cooled storage of elephant semen markedly reduces fertility. This study compared the effects on semen-quality parameters, including sperm DNA fragmentation, of storing elephant semen at 4\u00b0C or 15\u00b0C in a commonly-used diluent (TEST) or a diluent developed to protect against sperm DNA damage (BullMax). Storing elephant semen for &amp;gt;24 h in either extender at either temperature resulted in decreases in sperm motility, viability, acrosome integrity and DNA integrity (P &amp;lt; 0.05); the decrease in motility was especially rapid. A subjective impression of circular sperm movement in TEST was confirmed by a higher curvilinear velocity and amplitude of lateral head displacement, but lower straight-line velocity and linearity than in BullMax. Initial percentages of spermatozoa with fragmented DNA (%SDF) did not differ between extenders or temperatures, but the rate of increase in %SDF during a 48-h incubation at 37\u00b0C was higher in TEST than in BullMax (P &amp;lt; 0.05). In conclusion, BullMax allows more linear movement and better preserves DNA stability of stored elephant spermatozoa than TEST. Sperm DNA stability during incubation at 37\u00b0C is a promising, discriminative parameter for selecting semen storage conditions of bulls for elephant AI.<\/jats:p>","DOI":"10.1071\/rd11309","type":"journal-article","created":{"date-parts":[[2012,10,5]],"date-time":"2012-10-05T00:41:26Z","timestamp":1349397686000},"page":"1105-1116","source":"Crossref","is-referenced-by-count":21,"title":["Effect of cooled storage on quality and DNA integrity of Asian elephant (Elephas maximus) spermatozoa"],"prefix":"10.1071","volume":"24","author":[{"given":"P.","family":"Imrat","sequence":"first","affiliation":[{"name":"AFaculty of Veterinary Medicine, Department of Equine Sciences, Utrecht University, Yalelaan 114, 3584 CM, Utrecht, The Netherlands."},{"name":"GCorresponding author. 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