{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T04:58:47Z","timestamp":1761713927913,"version":"build-2065373602"},"reference-count":30,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4668,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,3]]},"abstract":"<jats:p>The in vitro quality of spermatozoa from one elephant (Elephas maximus) was examined after chilled storage and directional freezing (DF). High-quality, non-contaminated ejaculates (77.6 \u00b1 6.0% progressive motility, 3.9 \u00b1 1.5 \u00b5g creatinine mL\u20131 raw semen, 2.7 \u00b1 0.6% detached heads) were cryopreserved after 0 (0hStor), 12 (12hStor) and 24 h (24hStor) of chilled storage. At 0 h and 6 h post-thawing, total motility, plasma membrane integrity, acrosome integrity, mitochondrial activity and normal morphology were similar (P &amp;gt; 0.05) across treatments. In contrast, progressive motility, rapid velocity and several kinematic parameters were lower (P &amp;lt; 0.05) for 24Stor compared with 0hStor at 0 h post-thaw. By 6 h post-thaw, amplitude of lateral head displacement and velocity parameters (average pathway, straight-line and curvilinear velocity) were lower (P &amp;lt; 0.05) for 24hStor compared with 0hStor and 12hStor. DNA integrity was high and remained unchanged (P &amp;gt; 0.05) across all groups and processing stages (1.6 \u00b1 0.6% of cells contained fragmented DNA). Results indicate that DF after up to 12 h of chilled storage results in a post-thaw sperm population of acceptable quality for artificial insemination. These findings have implications for the cryopreservation of sex-sorted spermatozoa, which typically undergo more than 12 h of chilled storage prior to sorting and preservation.<\/jats:p>","DOI":"10.1071\/rd12129","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T21:39:05Z","timestamp":1344980345000},"page":"790-797","source":"Crossref","is-referenced-by-count":10,"title":["Characteristics of high-quality Asian elephant (Elephas maximus) ejaculates and in vitro sperm quality after prolonged chilled storage and directional freezing"],"prefix":"10.1071","volume":"25","author":[{"given":"J. K.","family":"O\u2019Brien","sequence":"first","affiliation":[{"name":"ASeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham St, San Diego, CA 92109, USA."},{"name":"ECorresponding author. Email: justine.obrien@seaworld.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. J.","family":"Steinman","sequence":"additional","affiliation":[{"name":"ASeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham St, San Diego, CA 92109, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. A.","family":"Montano","sequence":"additional","affiliation":[{"name":"ASeaWorld and Busch Gardens Reproductive Research Center, SeaWorld Parks and Entertainment, 2595 Ingraham St, San Diego, CA 92109, USA."},{"name":"BDepartment of Animal Science, Texas A&M University, College Station, TX 77843, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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