{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:04:12Z","timestamp":1791389052096,"version":"4.3.3"},"reference-count":21,"publisher":"CSIRO Publishing","issue":"2","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4443,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,6]]},"abstract":"<jats:p>In this study we evaluated the value of short-time insemination and early rescue intra-cytoplasmic sperm injection (ICSI) in preventing the occurrence of complete fertilisation failure for mild or moderate male infertility patients. A total of 866 couples with borderline semen who underwent in vitro fertilisation treatment in 2010 were included. Regular insemination was performed between January and June of 2010 and short-term insemination was performed from July through December 2010, where, as early as 4 h after insemination, oocytes were denuded from cumulus cells and extrusion of the second polar body was evaluated. Of the 4153 mature oocytes with a detectable second polar body 4 h after insemination, 3874 (93.3%) showed signs of fertilisation on Day 1. Where no second polar body was present in any of the retrieved oocytes for a given patient, rescue ICSI was performed immediately. Similar rates of normal fertilisation and percentage of good-quality embryos were obtained between early rescue ICSI and regular ICSI. Clinical pregnancy occurred in 16 of 43 patients (37.2%) receiving early rescue ICSI. Our results showed early rescue ICSI in combination with evaluation of the second polar body 4 h following insemination is an effective method to prevent complete fertilisation failure for patients with mild or moderate male infertility.<\/jats:p>","DOI":"10.1071\/rd12369","type":"journal-article","created":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T18:48:02Z","timestamp":1364410082000},"page":"346-350","source":"Crossref","is-referenced-by-count":19,"title":["The value of second polar body detection 4 hours after insemination and early rescue ICSI in preventing complete fertilisation failure in patients with borderline semen"],"prefix":"10.1071","volume":"26","author":[{"given":"Haixia","family":"Jin","sequence":"first","affiliation":[{"name":"AReproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimin","family":"Shu","sequence":"additional","affiliation":[{"name":"BDepartment of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanjun","family":"Dai","sequence":"additional","affiliation":[{"name":"AReproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Peng","sequence":"additional","affiliation":[{"name":"AReproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senlin","family":"Shi","sequence":"additional","affiliation":[{"name":"AReproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingpu","family":"Sun","sequence":"additional","affiliation":[{"name":"AReproductive Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China."},{"name":"CCorresponding author. 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