{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T02:11:14Z","timestamp":1767838274763,"version":"3.49.0"},"reference-count":57,"publisher":"CSIRO Publishing","issue":"2","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,23]]},"abstract":"<jats:p>In the first 2\u20133 weeks after parturition &amp;gt;90% of dairy cows will have some form of uterine infection. Uterine contamination with pathogens, such as Trueperella (formerly Arcanobacterium) pyogenes increases the risk of developing more severe endometritis, which can reduce conception rates. In this study, we compared the uterine proteome of cows infected with Trueperella pyogenes with that of uninfected cows, using 2D gel electrophoresis, and identified annexins A1 and A2 (ANXA1 and ANXA2), apolipoprotein A-1, calprotectin (S100A9), cathelicidin, enolase 1 (ENO1), peptidoglycan recognition protein 1 (PGLYRP1), phosphoglycerate mutase 1 (PGAM1), serine dehydratase (SDS) and serine protease inhibitors (SERPIN) B1, B3 and B4 proteins as differing in abundance in endometritis. Subsequently, levels of ten of these proteins were monitored in uterine samples collected from a herd of lactating, dairy cows at 15 and 42 days post-partum (DPP). The levels were compared with the cytology scores of the samples and the bacterial species isolated from the uterus. Cathelicidin, PGLYRP1, SERPINB1 and S100A9 levels at 15DPP showed strong positive correlations (r = 0.78, 0.80, 0.79, and 0.68 respectively; P &amp;lt; 0.001) with % of polymorphonuclear neutrophils (PMN). When compared with other bacterial pathogens identified, Streptococcus agalactiae and Truperella pyogenes induced increased expression of the indicator proteins, suggesting that these organisms may adversely affect the subsequent ability of the cow to conceive. Interestingly, there was no difference in the proportion of cows pregnant at 6 and 17 weeks after start of mating between the cows with high or low %PMN.<\/jats:p>","DOI":"10.1071\/rd13144","type":"journal-article","created":{"date-parts":[[2013,12,11]],"date-time":"2013-12-11T18:24:23Z","timestamp":1386786263000},"page":"395-406","source":"Crossref","is-referenced-by-count":15,"title":["Influence of pathogenic bacteria species present in the postpartum bovine uterus on proteome profiles"],"prefix":"10.1071","volume":"27","author":[{"given":"A. M.","family":"Ledgard","sequence":"first","affiliation":[{"name":"AAgResearch, Ruakura Research Centre, East Street, Hamilton 3240, New Zealand."},{"name":"BCorresponding author. 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