{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T10:51:04Z","timestamp":1775559064768,"version":"3.50.1"},"reference-count":38,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3793,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,18]]},"abstract":"<jats:p>This study evaluated the use of cooled semen in a fixed-time artificial insemination (FTAI) program compared with frozen\u2013thawed semen to improve pregnancy rates in beef cattle. Ejaculates of three bulls were collected and divided into two treatments: (1) frozen\u2013thawed semen and (2) cooled semen. Egg-yolk extender without glycerol was used for the cooled semen treatment. Straws (25 \u00d7 106 spermatozoa) were submitted to cooling for preservation at 5\u00b0C for 24 h, after which FTAI was performed. Nelore cows (n = 838) submitted to FTAI were randomly inseminated using frozen\u2013thawed semen or cooled semen. There was a 20% increase in the pregnancy per AI (P AI\u20131) using cooled semen compared with frozen\u2013thawed semen (59.9 \u00b1 4.7 vs 49.4 \u00b1 5.0%; P &amp;lt; 0.005). There was no difference in P AI\u20131 among the bulls (P = 0.40). The frozen\u2013thawed semen had fewer functional spermatozoa than did the cooled semen when evaluated by sperm motility (61.7 vs 81.0%), slow thermoresistance test (41.7 vs 66.7%) and hypoosmotic swelling test (38.3 vs 53.7%; P &amp;lt; 0.05). The percentage of sperm abnormalities did not differ between the freeze\u2013thawing and cooling processes (18.6 vs 22.1%; P &amp;gt; 0.05). Because there was less damage to spermatozoa and improvement in P AI\u20131, the use of cooled semen instead of frozen\u2013thawed semen is an interesting approach to increase reproductive efficiency in cattle submitted to a FTAI protocol.<\/jats:p>","DOI":"10.1071\/rd14185","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T19:55:59Z","timestamp":1420574159000},"page":"1004-1008","source":"Crossref","is-referenced-by-count":21,"title":["Cooled semen for fixed-time artificial insemination in beef cattle"],"prefix":"10.1071","volume":"28","author":[{"given":"Juliana C.","family":"Borges-Silva","sequence":"first","affiliation":[{"name":"AUniversity of S\u00e3o Paulo, Piracicaba, 13418-900, Brazil."},{"name":"DEmbrapa Pantanal, Corumb\u00e1, 79320-900, Brazil."},{"name":"FCorresponding author. Email: juliana.correa@embrapa.br"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio R.","family":"Silva","sequence":"additional","affiliation":[{"name":"BMelhore Animal Consultoria LTDA, Jaboticabal, 14871-835, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel B.","family":"Marinho","sequence":"additional","affiliation":[{"name":"CEma Agropecu\u00e1ria Pantanal LTDA, Corumb\u00e1, 79300-006, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eriklis","family":"Nogueira","sequence":"additional","affiliation":[{"name":"DEmbrapa Pantanal, Corumb\u00e1, 79320-900, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deiler C.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"EFederal University of Mato Grosso do Sul, Campo Grande, 79090-900, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Orc\u00edrio F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"DEmbrapa Pantanal, Corumb\u00e1, 79320-900, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urbano G. 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