{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:29:19Z","timestamp":1761719359127,"version":"build-2065373602"},"reference-count":39,"publisher":"CSIRO Publishing","issue":"10","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3722,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,18]]},"abstract":"<jats:p>Our previous study showed that the chemokine regulated upon activation normal T-cell expressed and secreted (RANTES) originating from the mouse epididymis bound to the midpiece of luminal spermatozoa. The present study was undertaken to investigate the association between RANTES and epididymal spermatozoa and to determine whether the association is mediated by the RANTES receptors CCR1, CCR3 or CCR5. The use of reverse transcription polymerase chain reaction (RT-PCR), immunohistochemical staining and immunofluorescent staining demonstrated that RANTES secreted by apical and narrow cells of mouse epididymal ducts was associated with luminal spermatozoa. Flow cytometric analysis and immunofluorescent labelling revealed that the association between RANTES and spermatozoa of different regions weakened gradually as the spermatozoa moved along the epididymis. Moreover, CCR1, CCR3 and CCR5 were expressed in epididymal spermatozoa and located on the head of epididymal spermatozoa, while RANTES was generally located at the midpiece. In conclusion, RANTES and its receptors were not in the same sperm location, suggesting that RANTES binding to mouse epididymal spermatozoa is independent of CCR1, CCR3 and CCR5.<\/jats:p>","DOI":"10.1071\/rd14231","type":"journal-article","created":{"date-parts":[[2015,3,18]],"date-time":"2015-03-18T22:32:33Z","timestamp":1426717953000},"page":"1509-1517","source":"Crossref","is-referenced-by-count":1,"title":["Different locations of RANTES and its receptors on mouse epididymal spermatozoa"],"prefix":"10.1071","volume":"28","author":[{"given":"Jin-Hua","family":"Wei","sequence":"first","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."},{"name":"EThese authors contributed equally to this work."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Feng","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."},{"name":"EThese authors contributed equally to this work."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"BResearch Center for Reproductive Medicine, 202 Hospital of PLA, No. 5, Guangrong Street, Shenyang 110003, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pang","family":"Cheng","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin-Fang","family":"Ma","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhao","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hang","family":"Dong","sequence":"additional","affiliation":[{"name":"CDepartment of Stomatology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan-Qiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."},{"name":"DCorresponding authors. Emails:"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"ADepartment of Human Anatomy, Histology and Embryology, The Fourth Military Medical University, No. 169, Changle West Road, Xi\u2019an 710032, China."},{"name":"DCorresponding authors. Emails:"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2015,3,19]]},"reference":[{"issue":"6","key":"2025102820164840300_R1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1002\/j.1939-4640.1999.tb03386.x","article-title":"Characterisation and development of the regional- and cellular-specific abnormalities in the epididymis of mice with beta-hexosaminidase A deficiency.","volume":"20","author":"Adamali","year":"1999","journal-title":"J. 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