{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:29:31Z","timestamp":1761719371430,"version":"build-2065373602"},"reference-count":35,"publisher":"CSIRO Publishing","issue":"9","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3728,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,7,21]]},"abstract":"<jats:p>This study investigated ways of improving the usefulness of ~1700 mL of poor-quality frozen semen collected from wild African elephant (Loxodonta africana) bulls. Ten semen samples from six bulls, frozen with 5% glycerol in Berliner cryomedium, with or without prior removal of the seminal plasma by centrifugation, were tested. All samples were subjected to the following density-gradient centrifugation treatments: no centrifugation (control), sham centrifugation, Percoll, OptiPrep, Isolate and PureSperm. Sample evaluation included motility, concentration, viability, acrosome integrity and normal morphology after thawing and after gradient centrifugation. Motility was also evaluated 3 h after thawing. While all treatments were similar to the Control in acrosome integrity and normal morphology, significant differences were noted in concentration, viability and motility. Samples treated by Percoll showed the best motility, which was maintained unchanged over 3 h of incubation (37\u00b0C). Correlations between manual and automated evaluations of concentration were high (cytometer; rho = 0.92), but were lower for viability (cytometer; rho = 0.57) and motility (computer-aided sperm analysis; rho = 0.66). By performing density centrifugation, the quality of these sperm samples may be improved to a level suitable for artificial insemination in elephants. Although a sizeable proportion of cells are lost in the process, combining samples may still allow for multiple inseminations.<\/jats:p>","DOI":"10.1071\/rd14378","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T19:42:14Z","timestamp":1426189334000},"page":"1433-1442","source":"Crossref","is-referenced-by-count":3,"title":["Sperm rescue in wild African elephants"],"prefix":"10.1071","volume":"28","author":[{"given":"Joseph","family":"Saragusty","sequence":"first","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."},{"name":"DCorresponding author. Email: saragusty@izw-berlin.de"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Prieto","sequence":"additional","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre","family":"Courtiol","sequence":"additional","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romain","family":"Potier","sequence":"additional","affiliation":[{"name":"BZooParc de Beauval, 41110 Saint Aignan s\/ Cher, France."},{"name":"CPresent address: FauneVet, CHV Atlantia, 22 rue Ren\u00e9 Viviani, 44200 Nantes, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"G\u00f6ritz","sequence":"additional","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.","family":"Hildebrandt","sequence":"additional","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Hermes","sequence":"additional","affiliation":[{"name":"ALeibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Stra\u00dfe 17, D-10315 Berlin, Germany."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2015,3,13]]},"reference":[{"key":"2025102820171105300_R1","first-page":"9","article-title":"Semen preservation for reproduction management in rare and endangered species.","volume":"s33","author":"Blottner","year":"1998","journal-title":"Adv. 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