{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:37:04Z","timestamp":1765827424437,"version":"build-2065373602"},"reference-count":32,"publisher":"CSIRO Publishing","issue":"2","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3463,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,12,3]]},"abstract":"<jats:p>Principles for selecting future research projects include interests of investigators, fundability, potential applications, ethical considerations, being able to formulate testable hypotheses and choosing the best models, including selection of the most appropriate species. The following 10 areas of assisted reproduction seem especially appropriate for further research: efficacious capacitation of bovine spermatozoa in vitro; improved in vitro bovine oocyte maturation; decreasing variability and increasing efficacy of bovine superovulation; improved fertility of sexed semen; improving equine IVF; improving cryopreservation of rooster spermatozoa; understanding differences between males in success of sperm cryopreservation and reasons for success in competitive fertilisation; mechanisms of reprogramming somatic cell nuclei after nuclear transfer; regulation of differentiation of ovarian primordial follicles; and means by which spermatozoa maintain fertility during storage in the epididymis. Issues are species specific for several of these topics, in most cases because the biology is species specific.<\/jats:p>","DOI":"10.1071\/rd15344","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T20:32:45Z","timestamp":1449088365000},"page":"125-129","source":"Crossref","is-referenced-by-count":4,"title":["Assisted reproduction with gametes and embryos: what research is needed and fundable?"],"prefix":"10.1071","volume":"28","author":[{"given":"George E.","family":"Seidel","sequence":"first","affiliation":[{"name":"1Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, CO 80523-1683, USA. Email: gseidel@colostate.edu"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2015,12,3]]},"reference":[{"key":"2025102820061499000_R1","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1095\/ BIOLREPROD30.4.926","article-title":"Inhibition of spermatozoa by caudal epididymal fluid: II. Interaction of pH and a quiescence factor.","volume":"30","author":"Acott","year":"1984","journal-title":"Biol. Reprod."},{"key":"2025102820061499000_R2","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1093\/MOLEHR\/GAT067","article-title":"Sperm capacitation: a distant landscape glimpsed but unexplored.","volume":"19","author":"Aitken","year":"2013","journal-title":"Mol. Hum. Reprod."},{"key":"2025102820061499000_R3","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1002\/MRD.1080410109","article-title":"Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes.","volume":"41","author":"Blondin","year":"1995","journal-title":"Mol. Reprod. Dev."},{"key":"2025102820061499000_R4","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/J.THERIOGENOLOGY.2012.10.024","article-title":"Seminal plasma effects on sex-sorting bovine sperm.","volume":"79","author":"Burroughs","year":"2013","journal-title":"Theriogenology"},{"key":"2025102820061499000_R5","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/J.THERIOGENOLOGY.2007.09.011","article-title":"The mare model for follicular maturation and reproductive aging in the woman.","volume":"69","author":"Carnevale","year":"2008","journal-title":"Theriogenology"},{"key":"2025102820061499000_R6","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/J.THERIOGENOLOGY.2014.05.011","article-title":"Use of a single injection of long-acting FSH to superovulate Holstein heifers: a preliminary study.","volume":"82","author":"Carvalho","year":"2014","journal-title":"Theriogenology"},{"key":"2025102820061499000_R7","doi-asserted-by":"publisher","first-page":"648","DOI":"10.1111\/J.1439-0531.2006.00837.X","article-title":"Successful low dose insemination of flow cytometrically sorted ram spermatozoa in sheep.","volume":"42","author":"de Graaf","year":"2007","journal-title":"Reprod. Domest. Anim."},{"key":"2025102820061499000_R8","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1113\/JPHYSIOL.1956.SP005447","article-title":"The effect of pituitary stalk section on light-induced oestrus in the ferret.","volume":"131","author":"Donovan","year":"1956","journal-title":"J. Physiol."},{"key":"2025102820061499000_R9","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/0378-4320(95)01463-2","article-title":"Factors that influence the proportion of offspring sired by a male after heterospermic insemination.","volume":"43","author":"Dziuk","year":"1996","journal-title":"Anim. Reprod. Sci."},{"key":"2025102820061499000_R10","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1016\/J.THERIOGENOLOGY.2011.09.025","article-title":"The mare: a 1000-pound guinea pig for study of the ovulatory follicular wave in women.","volume":"77","author":"Ginther","year":"2012","journal-title":"Theriogenology"},{"key":"2025102820061499000_R11","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1530\/JRF.0.0010283","article-title":"Physiological mechanisms concerned with conception: an inventory of unanswered questions.","volume":"1","author":"Hartman","year":"1960","journal-title":"J. Reprod. Fertil."},{"key":"2025102820061499000_R12","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/J.THERIOGENOLOGY.2013.09.010","article-title":"Forty years of embryo transfer in cattle: a review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces.","volume":"81","author":"Hasler","year":"2014","journal-title":"Theriogenology"},{"key":"2025102820061499000_R13","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1016\/S0093-691X(88)80038-4","article-title":"Fertilization rates in superovulating cows after deposition of semen on the infundibulum, near the uterotubal junction or after insemination with high numbers of sperm.","volume":"29","author":"Hawk","year":"1988","journal-title":"Theriogenology"},{"key":"2025102820061499000_R14","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1071\/RD12263","article-title":"Assisted reproduction techniques in the horse.","volume":"25","author":"Hinrichs","year":"2013","journal-title":"Reprod. Fertil. Dev."},{"key":"2025102820061499000_R15","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1016\/S0015-0282(16)37039-X","article-title":"Maturation and aging changes in rabbit spermatozoa isolated by ligatures at different levels of the epididymis.","volume":"20","author":"Igboeli","year":"1969","journal-title":"Fertil. Steril."},{"key":"2025102820061499000_R16","doi-asserted-by":"publisher","first-page":"9271","DOI":"10.1038\/SREP09271","article-title":"High survival of mouse oocytes\/embryos after vitrification without permeating cryoprotectants followed by ultra-rapid warming with an IR laser pulse.","volume":"5","author":"Jin","year":"2015","journal-title":"Sci. Rep."},{"key":"2025102820061499000_R17","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1530\/REP.1.01074","article-title":"TGF-beta superfamily members and ovarian follicle development.","volume":"132","author":"Knight","year":"2006","journal-title":"Reproduction"},{"key":"2025102820061499000_R18","doi-asserted-by":"publisher","DOI":"10.1111\/AND.12406","article-title":"High temperature is essential for preserved human sperm function during the devitrification process.","author":"Mansilla","year":"2015","journal-title":"Andrologia"},{"key":"2025102820061499000_R19","first-page":"47","article-title":"Centrifugation of stallion spermatozoa and its storage in large volume straws.","volume":"27","author":"Martin","year":"1979","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"2025102820061499000_R20","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1095\/BIOLREPROD.108.074880","article-title":"Hyperactivation of stallion sperm is required for successful in vitro fertilization of equine oocytes.","volume":"81","author":"McPartlin","year":"2009","journal-title":"Biol. Reprod."},{"key":"2025102820061499000_R21","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1530\/REP-11-0391","article-title":"FSH withdrawal improved developmental competence of oocytes in the bovine model.","volume":"143","author":"Nivet","year":"2012","journal-title":"Reproduction"},{"key":"2025102820061499000_R22","first-page":"375","article-title":"In vitro fertilization in the horse. A retrospective study.","volume":"44","author":"Palmer","year":"1991","journal-title":"J. Reprod. Fertil. Suppl."},{"key":"2025102820061499000_R23","doi-asserted-by":"publisher","first-page":"915","DOI":"10.3382\/PS.0750915","article-title":"Cryopreservation of rooster sperm using methyl cellulose.","volume":"75","author":"Phillips","year":"1996","journal-title":"Poult. Sci."},{"key":"2025102820061499000_R24","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1002\/JEZ.1402450114","article-title":"Comparison of heterospermic and homospermic insemination as measures of male fertility.","volume":"245","author":"Robl","year":"1988","journal-title":"J. Exp. Zool."},{"key":"2025102820061499000_R25","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1530\/JRF.0.0060049","article-title":"Composition of ram epididymal and testicular fluid and the biosynthesis of glycerylphosphorylcholine by the rabbit epididymis.","volume":"6","author":"Scott","year":"1963","journal-title":"J. Reprod. Fertil."},{"key":"2025102820061499000_R26","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/0093-691X(91)90156-8","article-title":"Embryo transfer: the next 100 years.","volume":"35","author":"Seidel","year":"1991","journal-title":"Theriogenology"},{"issue":"Suppl. s1","key":"2025102820061499000_R27","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1017\/S1751731114000202","article-title":"Update on sexed semen technology in cattle.","volume":"8","author":"Seidel","year":"2014","journal-title":"Animal"},{"key":"2025102820061499000_R28","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1146\/ANNUREV-ANIMAL-031412-103709","article-title":"Lessons from reproductive technology research.","volume":"3","author":"Seidel","year":"2015","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"2025102820061499000_R29","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1530\/REP.0.1240733","article-title":"Current status of sexing mammalian sperm.","volume":"124","author":"Seidel","year":"2002","journal-title":"Reproduction"},{"unstructured":"Utt, M. D. (2013). Relationships between in vivo and in vitro heterospermic ranking, embryo development, and sperm characteristic of Holstein and Jersey bulls. Ph.D. Thesis, The Ohio State University, Columbus, OH.","key":"2025102820061499000_R30"},{"key":"2025102820061499000_R31","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1530\/REP.1.01049","article-title":"Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm.","volume":"131","author":"Waterhouse","year":"2006","journal-title":"Reproduction"},{"key":"2025102820061499000_R32","doi-asserted-by":"publisher","first-page":"7298","DOI":"10.3168\/JDS.2014-8507","article-title":"Application of liquid semen technology improves conception rate of sex-sorted semen in lactating dairy cows.","volume":"97","author":"Xu","year":"2014","journal-title":"J. Dairy Sci."}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/28\/2\/125\/315535\/rd15344.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/28\/2\/125\/315535\/rd15344.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:07:22Z","timestamp":1761696442000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/28\/2\/125\/50530\/Assisted-reproduction-with-gametes-and-embryos"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,3]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,12,3]]}},"URL":"https:\/\/doi.org\/10.1071\/rd15344","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"type":"print","value":"1031-3613"},{"type":"electronic","value":"1448-5990"}],"subject":[],"published":{"date-parts":[[2015,12,3]]}}}