{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T03:23:43Z","timestamp":1774668223168,"version":"3.50.1"},"reference-count":76,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3282,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,6,19]]},"abstract":"<jats:p>The role of Ca2+ in sturgeon sperm maturation and motility was investigated. Sperm from mature male sterlets (Acipenser ruthenus) were collected from the Wolffian duct and testis 24 h after hormone induction. Testicular spermatozoa (TS) were incubated in Wolffian duct seminal fluid (WDSF) for 5 min at 20\u00b0C and were designated \u2018TS after IVM\u2019 (TSM). Sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. To investigate the role of calcium transport in the IVM process, IVM was performed (5 min at 20\u00b0C) in the presence of 2 mM EGTA, 100 \u00b5M Verapamil or 100 \u00b5M Tetracaine. No motility was observed in the case of TS (10 mM Tris, 25 mM NaCl, 50 mM Sucr with or without the addition of 2 mM EGTA). Both incubation of TS in WDSF and supplementation of the activation medium with Ca2+ led to sperm motility. The minimal Ca2+ concentration required for motility activation of Wolffian duct spermatozoa, TS and TSM was determined (1\u20132 nM for Wolffian duct spermatozoa and TSM; approximately 0.6 mM for TS). Motility was obtained after the addition of verapamil to the incubation medium during IVM, whereas the addition of EGTA completely suppressed motility, implying Ca2+ involvement in sturgeon sperm maturation. Further studies into the roles of Ca2+ transport in sturgeon sperm maturation and motility are required.<\/jats:p>","DOI":"10.1071\/rd16145","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T20:55:26Z","timestamp":1464728126000},"page":"1319-1328","source":"Crossref","is-referenced-by-count":8,"title":["Role of Ca2+ in the IVM of spermatozoa from the sterlet Acipenser ruthenus"],"prefix":"10.1071","volume":"29","author":[{"given":"Olga","family":"Bondarenko","sequence":"first","affiliation":[{"name":"AFaculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Z\u00e1ti\u0161\u00ed 728\/II, 389 25, Vodnany, Czech Republic."},{"name":"BCorresponding author. 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