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The aim of the present study was to investigate the effects of prostaglandin (PG) E2 and PGF2a (10, 50, 100, 250 and 500 ng mL\u20131) on the expression and secretion of adiponectin and its receptor genes and proteins by cultured in vitro porcine endometrial and myometrial tissues on Days 10\u201328 of pregnancy and Days 10\u201311 of the oestrous cycle. The gene expression was analysed using the real-time PCR method. Adiponectin protein secretion was determined by ELISA, whereas the receptors proteins content was defined using Western Blot analysis. Both PGE2 and PGF2a modulated the expression of adiponectin system genes and proteins in the uterus during early pregnancy. PGE2 and PGF2a had similar effects on the adiponectin system, which differed between the stages of gestation and between pregnancy and the oestrous cycle. On Days 10\u201311 of gestation, PGE2 and PGF2a generally increased adiponectin secretion by endometrial and myometrial tissues. Both PGs decreased levels of endometrial adiponectin receptor type 1 (AdipoR1), whereas only PGF2a decreased myometrial levels of AdipoR1. Both PGs increased myometrial adiponectin receptor type 2 (AdipoR2) levels. On Days 12\u201313 of gestation, PGE2 decreased AdipoR1 concentrations in both tissues and AdipoR2 levels in the endometrium. PGF2a decreased myometrial concentrations of both receptors. On Days 15\u201316 of gestation, both PGE2 and PGF2a increased concentrations of AdipoR1 and AdipoR2 in the endometrium and myometrium. PGE2 stimulated the secretion of adiponectin in the endometrium, but not in the myometrium. On Days 27\u201328 of pregnancy, both PGE2 and PGF2a inhibited the expression of AdipoR1 and AdipoR2 in endometrial and myometrial tissues and decreased the secretion of endometrial adiponectin. Both PGE2 and PGF2a had tissue-specific and dose-dependent effects on the adiponectin system.<\/jats:p>","DOI":"10.1071\/rd16181","type":"journal-article","created":{"date-parts":[[2016,12,11]],"date-time":"2016-12-11T23:32:44Z","timestamp":1481499164000},"page":"1832-1845","source":"Crossref","is-referenced-by-count":3,"title":["Modulation of adiponectin system expression in the porcine uterus during early pregnancy by prostaglandin E2 and F2a"],"prefix":"10.1071","volume":"29","author":[{"given":"Kamil","family":"Dobrzyn","sequence":"first","affiliation":[{"name":"ADepartment of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Smolinska","sequence":"additional","affiliation":[{"name":"ADepartment of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland."},{"name":"BCorresponding author. 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