{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T04:52:55Z","timestamp":1761713575050,"version":"build-2065373602"},"reference-count":60,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":2800,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,3,16]]},"abstract":"<jats:p>Infection with Gram-negative bacteria is a major cause of aberrant inflammation in the oviduct; consequences can include tubal-based infertility and\/or ectopic pregnancy. Understanding the inflammatory response is necessary for the development of novel treatment options that counter inflammation-induced infertility. The aim of the present study was to determine the effect of intraperitoneal (i.p.) administration of Escherichia coli-derived lipopolysaccharide (LPS) on the acute expression of inflammatory mRNAs and microRNAs (miRNAs) in the oviduct. On the day of oestrus, 6- to 8-week-old CD1 mice were injected i.p. with 0, 2 or 10 \u00b5g LPS in 100 \u00b5L phosphate-buffered saline. Mice were killed 24 h later and the oviducts collected for gene expression analyses. The effect of treatment on the expression of mRNAs and miRNAs was evaluated by one-way analysis of variance (ANOVA), with treatment means of differentially expressed (P &amp;lt; 0.05) transcripts separated using Scheff\u00e9\u2019s test. LPS treatment affected 49 of 179 targeted inflammatory mRNAs and 51 of 578 miRNAs (P &amp;lt; 0.05). The identity of differentially expressed miRNAs predicted as regulators of chemokine and interleukin ligand mRNAs was then extracted using the microRNA.org database. The results of the present study indicate that systemic treatment with LPS induces a robust inflammatory response in the oviducts of mice, and identify key mRNAs and putative miRNAs modulating this effect.<\/jats:p>","DOI":"10.1071\/rd17241","type":"journal-article","created":{"date-parts":[[2017,9,25]],"date-time":"2017-09-25T18:56:35Z","timestamp":1506365795000},"page":"600-608","source":"Crossref","is-referenced-by-count":1,"title":["Effect of lipopolysaccharide on the expression of inflammatory mRNAs and microRNAs in the mouse oviduct"],"prefix":"10.1071","volume":"30","author":[{"given":"Katheryn L.","family":"Cerny","sequence":"first","affiliation":[{"name":"ADepartment of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanne A. 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