{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T13:29:25Z","timestamp":1781530165063,"version":"3.54.1"},"reference-count":57,"publisher":"CSIRO Publishing","issue":"9","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":2618,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,8,13]]},"abstract":"<jats:p>The present study evaluated the effect of four ovarian stimulation protocols on the follicular population and molecular status of cumulus\u2013oocyte complexes (COCs). Twelve Santa In\u00eas ewes (in a cross-over design) received 80 or 120 mg FSH alone in a multiple-dose (MD80 and MD120) regimen or in combination with 300 IU equine chorionic gonadotrophin (eCG) in a one-shot (OS80 and OS120) protocol. The follicular population, COC recovery rate, mean COCs per ewe and the rate of brilliant Cresyl blue-positive (BCB+) COCs were similar among treatments (P &amp;gt; 0.05). The expression of markers of oocyte competence (ZAR1, zygote arrest 1; MATER, maternal antigen that embryo requires; GDF9, growth differentiation factor 9; BMP15, bone morphogenetic protein 15; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2 associated X protein) and the steroidogenic pathway (ERa, oestrogen receptor a; LHr, LH receptor; FSHr, FSH receptor; STAR, steroidogenic acute regulatory protein) was affected by stimulation. Specifically, the expression of markers of the steroidogenic pathway was reduced with increasing FSH dose in the OS protocol. FSH at a dose of 80 mg reduced the expression of FSHr and ERa in the OS versus MD protocol. Conversely, in MD protocol, only LHr was affected by increasing FSH dose. In conclusion, 80 mg FSH in the MD or OS protocol was sufficient to promote the development of multiple follicles and obtain fully grown (BCB+) oocytes. The MD protocol may be more appropriate for the production of better-quality oocytes.<\/jats:p>","DOI":"10.1071\/rd17337","type":"journal-article","created":{"date-parts":[[2018,3,26]],"date-time":"2018-03-26T18:22:56Z","timestamp":1522088576000},"page":"1234-1244","source":"Crossref","is-referenced-by-count":19,"title":["Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus\u2013oocyte complexes"],"prefix":"10.1071","volume":"30","author":[{"given":"Gl\u00e1ucia M.","family":"Bragan\u00e7a","sequence":"first","affiliation":[{"name":"AFaculdade de Veterin\u00e1ria, Universidade Federal Fluminense, Rua Vital Brazil, 64, CEP 24320-340, Niteroi, RJ, Brazil."},{"name":"DCorresponding author. Email: glauciaveterinaria@yahoo.com.br"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ribrio Ivan T. 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