{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T09:56:40Z","timestamp":1792144600579,"version":"4.4.0"},"reference-count":20,"publisher":"CSIRO Publishing","issue":"12","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":2531,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,10,30]]},"abstract":"<jats:p>Polycystic ovary syndrome (PCOS), a familial aggregation disease that causes anovulation in women, has well-recognised characteristics, two of which are hyperinsulinaemia and hyperandrogenaemia. To determine whether the DNA methylation status is altered in oocytes by high insulin and androgen levels, we generated a mouse model with hyperinsulinaemia and hyperandrogenaemia by injection of insulin and human chorionic gonadotrophin and investigated DNA methylation changes through single-cell level whole genome bisulphite sequencing. Our results showed that hyperinsulinaemia and hyperandrogenaemia had no significant effects on the global DNA methylation profile and different functional regions of genes, but did alter methylation status of some genes, which were significantly enriched in 17 gene ontology (GO) terms (P &amp;lt; 0.05) by GO analysis. Among differently methylated genes, some were related to the occurrence of PCOS. Based on our results, we suggest that hyperinsulinaemia and hyperandrogenaemia may cause changes in some DNA methylation loci in oocytes.<\/jats:p>","DOI":"10.1071\/rd18002","type":"journal-article","created":{"date-parts":[[2018,6,22]],"date-time":"2018-06-22T01:58:01Z","timestamp":1529632681000},"page":"1713-1719","source":"Crossref","is-referenced-by-count":14,"title":["The DNA methylation profile of oocytes in mice with hyperinsulinaemia and hyperandrogenism as detected by single-cell level whole genome bisulphite sequencing (SC-WGBS) technology"],"prefix":"10.1071","volume":"30","author":[{"given":"Qian-Nan","family":"Li","sequence":"first","affiliation":[{"name":"ACollege of Life Science, Northeast Agricultural University, #600 Changjiang Road, Harbin 150030, China."},{"name":"BState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, #1 Beichen West Road, Beijing 100101, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Guo","sequence":"additional","affiliation":[{"name":"CFertility Preservation Lab, Reproductive Medicine Centre, Guangdong Second Provincial General Hospital, The Second Peoples\u2019 Hospital of Guangdong Province, #466 Xingang Road, Guangzhou 510317, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Hou","sequence":"additional","affiliation":[{"name":"BState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, #1 Beichen West Road, Beijing 100101, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Hong","family":"Ou","sequence":"additional","affiliation":[{"name":"CFertility Preservation Lab, Reproductive Medicine Centre, Guangdong Second Provincial General Hospital, The Second Peoples\u2019 Hospital of Guangdong Province, #466 Xingang Road, Guangzhou 510317, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhonghua","family":"Liu","sequence":"additional","affiliation":[{"name":"ACollege of Life Science, Northeast Agricultural University, #600 Changjiang Road, Harbin 150030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing-Yuan","family":"Sun","sequence":"additional","affiliation":[{"name":"ACollege of Life Science, Northeast Agricultural University, #600 Changjiang Road, Harbin 150030, China."},{"name":"BState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, #1 Beichen West Road, Beijing 100101, China."},{"name":"DQingdao Agricultural University, #700 Changcheng Road, Qingdao 266109, China."},{"name":"ECorresponding author. 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