{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T18:18:18Z","timestamp":1789323498069,"version":"build-2803163510"},"reference-count":46,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":1950,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,2]]},"abstract":"<jats:p>This study compared the morphometric, subcellular characteristics, invitro fertilisation (IVF) and embryonic developmental potential of metaphase II (MII) mouse oocytes obtained from females superovulated with either anti-inhibin serum\u2013human chorionic gonadotrophin (AIS-hCG) or pregnant mare serum gonadotrophin (PMSG)-hCG. The oocyte\u2019s quantity, quality, zona pellucida (ZP) thickness, perivitelline space (PVS), diameter, microtubules, F-actin, cortical granules (CGs) and mitochondrial distribution were determined. Superovulation using AIS-hCG resulted in a higher numbers of oocyte\/donor compared with PMSG-hCG (P\u00a0=\u00a00.002). There was no difference in morphologically normal and abnormal oocytes between AIS-hCG and PMSG-hCG (P\u00a0=\u00a00.425 and P\u00a0=\u00a00.194, respectively). The morphometric measurements showed no difference in oocyte diameter between AIS-hCG and PMSG-hCG (P\u00a0=\u00a00.289). However, the thickness of the ZP of oocytes from AIS-hCG females was decreased compared with PMSG-hCG (P\u00a0&amp;lt;\u00a00.001). The PVS of oocytes from the AIS-hCG was larger than with PMSG-hCG (P\u00a0&amp;lt;\u00a00.001). The microtubules of oocytes from both AIS-hCG and PMSG-hCG were normal, although there was an increased fluorescence intensity in the AIS-hCG oocytes (P\u00a0&amp;lt;\u00a00.001). The F-actin and CGs distribution in oocytes from both AIS-hCG and PMSG-hCG were similar (P\u00a0=\u00a00.330 and P\u00a0=\u00a00.13, respectively). Although the oocytes from PMSG-hCG females had homogenously distributed mitochondria, AIS-hCG oocytes showed more peripheral distribution with no differences in fluorescence intensity (P\u00a0=\u00a00.137). The blastocyst development rates after IVF with fresh sperm showed no difference between AIS-hCG and PMSG-hCG (P\u00a0=\u00a00.235). These data suggested that AIS-hCG superovulation produces high numbers of morphologically normal oocytes that also possess normal subcellular structures, good morphological characteristics and had high in\u00a0vitro embryonic developmental potential.<\/jats:p>","DOI":"10.1071\/rd19131","type":"journal-article","created":{"date-parts":[[2020,1,23]],"date-time":"2020-01-23T17:56:31Z","timestamp":1579802191000},"page":"474-483","source":"Crossref","is-referenced-by-count":5,"title":["Morphometric, subcellular, in\u00a0vitro fertilisation and embryonic developmental assessment of mouse oocytes produced by anti-inhibin serum or pregnant mare serum gonadotrophin superovulation"],"prefix":"10.1071","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8820-139X","authenticated-orcid":true,"given":"Liga","family":"Wuri","sequence":"first","affiliation":[{"name":"ADivision of Animal Sciences, University of Missouri, 920 East Campus Drive, Columbia, MO 65201, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cansu","family":"Agca","sequence":"additional","affiliation":[{"name":"BCollege of Veterinary Medicine, University of Missouri, 1600 East Rollins Street, Columbia, MO 65211, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuksel","family":"Agca","sequence":"additional","affiliation":[{"name":"ADivision of Animal Sciences, University of Missouri, 920 East Campus Drive, Columbia, MO 65201, USA."},{"name":"BCollege of Veterinary Medicine, University of Missouri, 1600 East Rollins Street, Columbia, MO 65211, USA."},{"name":"CCorresponding author. 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