{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:02:28Z","timestamp":1761717748046,"version":"build-2065373602"},"reference-count":53,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":2031,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,22]]},"abstract":"<jats:p>Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disease caused by a deficiency of the lysosomal hydrolase, \u03b1-L-iduronidase (IDUA). IDUA degrades heparan and dermatan sulfates, two types of glycosaminoglycan (GAG), important signalling and structural molecules of the extracellular matrix. Because many cell types store GAGs, MPS I has been investigated in human and animal models. Enzyme replacement therapy is available for MPS I patients and has improved their life expectancy, allowing them to achieve reproductive age. The aim of this study was to evaluate epididymal and sperm morphology and function in a murine model of MPS I. We used C57BL Idua+\/+ and Idua\u2212\/\u2212 adult male mice (6 months old) to investigate epididymal morphology, sperm ultrastructure, GAG characterisation and mating competence. Epithelial GAG storage, especially in the cauda epididymidis, was seen in Idua\u2212\/\u2212 mice. Regardless of the morphologic change and GAG storage found in the cauda epididymis, sperm morphology and motility were normal, similar to wild types. In the interstitium, vacuolated cells were found in addition to deposits of GAGs. Mating was not impaired in Idua\u2212\/\u2212 males and litter sizes were similar between groups. At the time point of the disease evaluated, the deficiency in IDUA affected the morphology of the epididymis in male Idua\u2212\/\u2212 mice, whereas sperm appearance and motility and the male\u2019s capacity to mate and impregnate females were preserved.<\/jats:p>","DOI":"10.1071\/rd19144","type":"journal-article","created":{"date-parts":[[2019,11,3]],"date-time":"2019-11-03T23:13:17Z","timestamp":1572822797000},"page":"304-312","source":"Crossref","is-referenced-by-count":5,"title":["Evidence that glycosaminoglycan storage and collagen deposition in the cauda epididymidis does not impair sperm viability in the Mucopolysaccharidosis type I mouse model"],"prefix":"10.1071","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-6012","authenticated-orcid":true,"given":"Cinthia Castro","family":"do Nascimento","sequence":"first","affiliation":[{"name":"ADepartment of Psychobiology, Universidade Federal de S\u00e3o Paulo, 04024-002, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odair","family":"Aguiar","sequence":"additional","affiliation":[{"name":"BDepartment of Biosciences, Universidade Federal de S\u00e3o Paulo, 11015-020, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo Monteiro","family":"Viana","sequence":"additional","affiliation":[{"name":"CDepartment of Biochemistry, Universidade Federal de S\u00e3o Paulo, 04044-020, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e2nia","family":"D\u2019Almeida","sequence":"additional","affiliation":[{"name":"ADepartment of Psychobiology, Universidade Federal de S\u00e3o Paulo, 04024-002, Brazil."},{"name":"DCorresponding author. 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