{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:20:42Z","timestamp":1790162442169,"version":"4.1.0"},"reference-count":41,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":1888,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,22]]},"abstract":"<jats:p>Long non-coding (lnc) RNAs are a series of RNAs longer than 200 nucleotides that do not code for protein products. Whole-genome expression profiles of lncRNAs suggest that they play important roles in spermatogenesis because they are particularly abundant in testes. However, most of their characteristics and functions remain unclear. The aim of this study was to define the function of lncRNA5512, which is abundant in spermatocytes and round spermatids, in mouse fertility in\u00a0vivo. To investigate this we generated lncRNA5512-knockout mice by clustered regularly interspaced palindromic repeats (CRISPR)\/CRISPR-associated protein (Cas) 9 technology. Knockout mice showed normal spermatogenesis and fertility, and had no detectable abnormalities. This indicates that lncRNA5512 does not affect mouse fertility despite its high expression in the testes. Its specific localisation in spermatocytes and round spermatids suggests that it could be a useful marker for the identification of spermatocytes and round spermatids in mouse testes.<\/jats:p>","DOI":"10.1071\/rd19246","type":"journal-article","created":{"date-parts":[[2020,3,25]],"date-time":"2020-03-25T20:17:10Z","timestamp":1585167430000},"page":"706-713","source":"Crossref","is-referenced-by-count":14,"title":["Deletion of lncRNA5512 has no effect on spermatogenesis and reproduction in mice"],"prefix":"10.1071","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-0174","authenticated-orcid":true,"given":"Yu","family":"Zhu","sequence":"first","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Lin","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"He","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanshu","family":"Wang","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shitao","family":"Chen","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Li","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Song","sequence":"additional","affiliation":[{"name":"BShanghai Key Laboratory of Reproductive Medicine, School of Medicine, Shanghai Jiao Tong University, 280 South Chongqing Road, Huangpu District, Shanghai 200025, China."},{"name":"CCorresponding authors. Email: sunfei@shsmu.edu.cn; ningsong@shsmu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0870-8375","authenticated-orcid":true,"given":"Fei","family":"Sun","sequence":"additional","affiliation":[{"name":"AInternational Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, 910 Hengshan Road, Xuhui District, Shanghai 200030, China."},{"name":"CCorresponding authors. 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