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In Experiment 1, delaying spontaneous resumption of meiosis through the process of simulated physiological oocyte maturation with the addition of the adenylate cyclase activator forskolin (50 \u00b5M) and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (100 \u00b5M) to overnight holding medium before maturation improved blastocyst production (P &amp;lt; 0.05). In Experiment 2, the blastocyst production rate was increased significantly when cumulin (100 ng mL\u22121) was added to the overnight holding or culture media (P &amp;lt; 0.05). In Experiment 3, immature oocytes held overnight at 16\u00b0C before maturation had improved developmental competence than those held at 20\u00b0C and 5\u00b0C (P &amp;lt; 0.05). There was no difference between maturation rates, but blastocyst formation per cleaved oocyte was significantly greater in oocytes held overnight at 16\u00b0C than at 20\u00b0C or 5\u00b0C. Furthermore, blastocyst formation per recovered oocyte and per fertilised oocyte was greater when oocytes were held before maturation at 16\u00b0C than at 5\u00b0C (P &amp;lt; 0.05). In Experiment 4, the addition of sodium ascorbate (AC; 50 \u00b5g mL\u22121) to the maturation and\/or culture media of oocytes and IVP embryos did not improve blastocyst production, but did appear to lower cleavage rates compared with oocytes and embryos cultured without AC.<\/jats:p>","DOI":"10.1071\/rd19249","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T20:24:05Z","timestamp":1594239845000},"page":"1012-1021","source":"Crossref","is-referenced-by-count":14,"title":["Conditions to optimise the developmental competence of immature equine oocytes*"],"prefix":"10.1071","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0601-799X","authenticated-orcid":true,"given":"Elizabeth S.","family":"Metcalf","sequence":"first","affiliation":[{"name":"AHonahlee PC, 14005 SW Tooze Road, Sherwood, OR 97140, USA."},{"name":"BAndrology Division, Department of Obstetrics and Gynecology, Oregon Health and Science University School of Medicine, 3303\u2009S. 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