{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T06:09:17Z","timestamp":1761718157309,"version":"build-2065373602"},"reference-count":61,"publisher":"CSIRO Publishing","issue":"8","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":1863,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,11]]},"abstract":"<jats:p>Kisspeptin1 (Kiss1), a product of the Kiss1 gene, plays an important role in the regulation of reproduction in vertebrates by activating the Kiss1 receptor (Kiss1R) and its coexpression with gonadotrophin-releasing hormone (GnRH) in GnRH neurons. The purpose of this study was to clone the Kiss1 and Kiss1R genes found in the brain of Alligator sinensis and to explore their relationship with reproduction. The full-length cDNA of Kiss1 is 816\u00a0bp, the open reading frame (ORF) is 417\u00a0bp and the gene encodes a 138-amino acid precursor protein. The full-length cDNA of Kiss1R is 2348\u00a0bp, the ORF is 1086\u00a0bp and the gene encodes a 361-amino acid protein. Quantitative polymerase chain reaction showed that, except for Kiss1R expression in the hypothalamus, the expression of Kiss1 and Kiss1Rduring the reproductive period of A. sinensis was higher than that in the hypothalamus, pituitary gland and ovary during the hibernation period. The changes in GnRH2 mRNA in the hypothalamus were similar to those of GnRH1 and peaked during the reproductive period. This study confirms the existence of Kiss1 and Kiss1R in A. sinensis and the findings strongly suggest that Kiss1 and Kiss1R may participate in the regulation of GnRH secretion in the hypothalamus of alligators during the reproductive period. Furthermore, this is the first report of the full-length cDNA sequences of Kiss1 and Kiss1R in reptiles.<\/jats:p>","DOI":"10.1071\/rd19332","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T02:43:05Z","timestamp":1587350585000},"page":"792-804","source":"Crossref","is-referenced-by-count":9,"title":["Cloning, characterisation and expression profile of kisspeptin1 and the kisspeptin1 receptor in the hypothalamic\u2013pituitary\u2013ovarian axis of Chinese alligator Alligator sinensis during the reproductive cycle"],"prefix":"10.1071","volume":"32","author":[{"given":"Ruidong","family":"Zhang","sequence":"first","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."},{"name":"BCollege of Life Sciences, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2332-6391","authenticated-orcid":true,"given":"Haitao","family":"Nie","sequence":"additional","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulong","family":"Duan","sequence":"additional","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Yan","sequence":"additional","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Izaz","sequence":"additional","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renping","family":"Wang","sequence":"additional","affiliation":[{"name":"CAlligator Research Center of Anhui Province, Xuanzhou 242000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongkang","family":"Zhou","sequence":"additional","affiliation":[{"name":"CAlligator Research Center of Anhui Province, Xuanzhou 242000, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-3908","authenticated-orcid":true,"given":"Xiaobing","family":"Wu","sequence":"additional","affiliation":[{"name":"AKey Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241000, China."},{"name":"DCorresponding author. Email:wuxb@mail.ahnu.edu"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"2025102820104440400_R1","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/J.YGCEN.2013.03.030","article-title":"Expression of kisspeptins and their receptors, gnrh-1\/gnrhr-II-1a and gonadotropin genes in the brain of adult male and female European sea bass during different gonadal stages.","volume":"187","author":"Alvarado","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R2","doi-asserted-by":"publisher","first-page":"1218","DOI":"10.1210\/EN.2014-1915","article-title":"Kisspeptin: beyond the brain.","volume":"156","author":"Bhattacharya","year":"2015","journal-title":"Endocrinology"},{"key":"2025102820104440400_R3","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1016\/J.FERTNSTERT.2012.02.021","article-title":"Analysis of the expression of neurokinin B, kisspeptin, and their cognate receptors NK3R and KISS1R in the human female genital tract.","volume":"97","author":"Cejudo-Rom\u00e1n","year":"2012","journal-title":"Fertil. Steril."},{"key":"2025102820104440400_R4","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1095\/BIOLREPROD.112.103515","article-title":"Kisspeptin receptor, gpr54, as a candidate for the regulation of testicular activity in the frog Rana esculenta.","volume":"88","author":"Chianese","year":"2013","journal-title":"Biol. Reprod."},{"key":"2025102820104440400_R5","doi-asserted-by":"publisher","first-page":"727","DOI":"10.3389\/FENDO.2018.00727","article-title":"Editorial: the multiple facets of kisspeptin activity in biological systems.","volume":"9","author":"Chianese","year":"2018","journal-title":"Front. Endocrinol."},{"key":"2025102820104440400_R6","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1046\/J.1365-2826.1999.00390.X","article-title":"Fos localization in cytosolic and nuclear compartments in neurones of the frog, Rana esculenta, brain: an analysis carried out in parallel with GnRH molecular forms.","volume":"11","author":"Cobellis","year":"1999","journal-title":"J. Neuroendocrinol."},{"key":"2025102820104440400_R7","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1152\/PHYSIOL.00009.2010","article-title":"The role of kisspeptinsignaling in reproduction.","volume":"25","author":"D\u2019Anglemont","year":"2010","journal-title":"Physiology (Bethesda)"},{"key":"2025102820104440400_R8","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1159\/000215930","article-title":"Kisspeptin-like immunoreactive neuron distribution in the green anole (Anolis carolinensis).","volume":"73","author":"Dunham","year":"2009","journal-title":"Brain Behav. Evol."},{"key":"2025102820104440400_R9","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/J.YGCEN.2015.07.015","article-title":"Molecular characterization of the gonadal kisspeptin system: cloning, tissue distribution, gene expression analysis and localization in sablefish (Anoplopoma fimbria).","volume":"225","author":"Fairgrieve","year":"2016","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R10","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/J.MCE.2008.11.017","article-title":"Evidence for two distinct KiSS genes in non-placental vertebrates that encode kisspeptins with different gonadotropin-releasing activities in fish and mammals.","volume":"312","author":"Felip","year":"2009","journal-title":"Mol. Cell. Endocrinol."},{"key":"2025102820104440400_R11","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1016\/J.BBRC.2003.11.066","article-title":"The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system.","volume":"312","author":"Funes","year":"2003","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2025102820104440400_R12","doi-asserted-by":"publisher","first-page":"E520","DOI":"10.1152\/AJPENDO.90895.2008","article-title":"KiSS-1 in the mammalian ovary: distribution of kisspeptin in human and marmoset and alterations in KiSS-1 mRNA levels in a rat model of ovulatory dysfunction.","volume":"296","author":"Gayt\u00e1n","year":"2009","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"2025102820104440400_R13","doi-asserted-by":"publisher","first-page":"3088","DOI":"10.1210\/EN.2014-1110","article-title":"Kisspeptin receptor haplo-insufficiency causes premature ovarian failure despite preserved gonadotropin secretion.","volume":"155","author":"Gaytan","year":"2014","journal-title":"Endocrinology"},{"key":"2025102820104440400_R14","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/J.YGCEN.2010.07.018","article-title":"Social status regulates kisspeptin receptor mRNA in the brain of Astatotilapiaburtoni.","volume":"169","author":"Grone","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R15","doi-asserted-by":"publisher","first-page":"11349","DOI":"10.1523\/JNEUROSCI.3328-05.2005","article-title":"Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty.","volume":"25","author":"Han","year":"2005","journal-title":"J. Neurosci."},{"key":"2025102820104440400_R16","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1210\/EN.2009-0552","article-title":"Distribution and postnatal development of Gpr54 gene expression in mouse brain and gonadotropin-releasing hormone neurons.","volume":"151","author":"Herbison","year":"2010","journal-title":"Endocrinology"},{"key":"2025102820104440400_R17","doi-asserted-by":"publisher","first-page":"E520","DOI":"10.1111\/JNE.12356","article-title":"Mapping of kisspeptin receptor mRNA in the whole rat brain and its co-localisation with oxytocin in the paraventricular nucleus.","volume":"28","author":"Higo","year":"2016","journal-title":"J. Neuroendocrinol."},{"key":"2025102820104440400_R18","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/J.CBPA.2016.07.015","article-title":"Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages.","volume":"203","author":"Imamura","year":"2017","journal-title":"Comp. Biochem. Physiol., Part A Mol. Integr. Physiol."},{"key":"2025102820104440400_R19","doi-asserted-by":"publisher","first-page":"2467","DOI":"10.1210\/EN.2007-1503","article-title":"Identification of KiSS-1 product kisspeptin and steroid-sensitive sexually dimorphic kisspeptin neurons in medaka (Oryziaslatipes).","volume":"149","author":"Kanda","year":"2008","journal-title":"Endocrinology"},{"key":"2025102820104440400_R20","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1111\/J.1365-2826.2012.02296.X","article-title":"Steroid sensitive kiss2 of side-blotched in the goldfish: evolutionary insights into the duplicate kisspeptin gene-expressing neurones.","volume":"24","author":"Kanda","year":"2012","journal-title":"J. Neuroendocrinol."},{"key":"2025102820104440400_R21","doi-asserted-by":"publisher","first-page":"34631","DOI":"10.1074\/JBC.M104847200","article-title":"The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54.","volume":"276","author":"Kotani","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2025102820104440400_R22","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1093\/JNCI\/88.23.1731","article-title":"KiSS-1, a novel human malignant melanoma metastasis-suppressor gene.","volume":"88","author":"Lee","year":"1996","journal-title":"J. Natl Cancer Inst."},{"key":"2025102820104440400_R23","doi-asserted-by":"publisher","first-page":"2837","DOI":"10.1210\/EN.2008-1679","article-title":"Molecular evolution of multiple forms of kisspeptins and GPR54 receptors in vertebrates.","volume":"150","author":"Lee","year":"2009","journal-title":"Endocrinology"},{"key":"2025102820104440400_R24","doi-asserted-by":"publisher","first-page":"193","DOI":"10.3803\/ENM.2016.31.2.193","article-title":"Kisspeptinregulation of neuronal activity throughout the central nervous system.","volume":"31","author":"Liu","year":"2016","journal-title":"Endocrinol. Metab."},{"key":"2025102820104440400_R25","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1210\/EN.2010-1142","article-title":"Kisspeptin regulates gonadotroph and somatotroph function in nonhuman primate pituitary via common and distinct signaling mechanisms.","volume":"152","author":"Luque","year":"2011","journal-title":"Endocrinology"},{"key":"2025102820104440400_R26","doi-asserted-by":"publisher","first-page":"2813","DOI":"10.1210\/EN.2008-1698","article-title":"Biological and anatomical evidence for kisspeptin regulation of the hypothalamic\u2013pituitary\u2013gonadal axis of estrous horse mares.","volume":"150","author":"Magee","year":"2009","journal-title":"Endocrinology"},{"key":"2025102820104440400_R27","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/J.YGCEN.2003.09.017","article-title":"Jun localization in cytosolic and nuclear compartments in brain-pituitary system of the frog, Rana esculenta: an analysis carried out in parallel with GnRH molecular forms during the annual reproductive cycle.","volume":"135","author":"Meccariello","year":"2004","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R28","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/J.YGCEN.2012.07.033","article-title":"Kisspeptin and seasonal control of reproduction in male European sea bass (Dicentrarchuslabrax).","volume":"179","author":"Migaud","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R29","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/J.PEPTIDES.2008.04.015","article-title":"Molecular cloning of the bullfrog kisspeptin receptor GPR54 with high sensitivity to Xenopuskisspeptin.","volume":"30","author":"Moon","year":"2009","journal-title":"Peptides"},{"key":"2025102820104440400_R30","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/J.YHBEH.2016.12.006","article-title":"The role of the kisspeptin system in regulation of the reproductive endocrine axis and territorial behavior in male side-blotched lizards (Uta stansburiana).","volume":"89","author":"Neuman-Lee","year":"2017","journal-title":"Horm. Behav."},{"key":"2025102820104440400_R31","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1210\/ER.2009-0005","article-title":"Kisspeptinsignaling in the brain.","volume":"30","author":"Oakley","year":"2009","journal-title":"Endocr. Rev."},{"key":"2025102820104440400_R32","doi-asserted-by":"publisher","first-page":"4270","DOI":"10.1210\/EN.2012-2267","article-title":"Molecular evolution of kiss2 genes and peptides in vertebrates.","volume":"154","author":"Osugi","year":"2013","journal-title":"Endocrinology"},{"key":"2025102820104440400_R33","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1471-2148-14-30","article-title":"Looking for the bird kiss: evolutionary scenario in sauropsids.","volume":"14","author":"Pasquier","year":"2014","journal-title":"BMC Evol. Biol."},{"key":"2025102820104440400_R34","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/S0079-6123(08)81005-9","article-title":"Chapter 5:physiological roles of the kisspeptin\/GPR54 system in the neuroendocrine control of reproduction.","volume":"181","author":"Pineda","year":"2010","journal-title":"Prog. Brain Res."},{"key":"2025102820104440400_R35","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1152\/PHYSREV.00037.2010","article-title":"Kisspeptins and reproduction: physiological roles and regulatory mechanisms.","volume":"92","author":"Pinilla","year":"2012","journal-title":"Physiol. Rev."},{"key":"2025102820104440400_R36","doi-asserted-by":"publisher","first-page":"5927","DOI":"10.1210\/EN.2010-0565","article-title":"Kisspeptin-10 inhibits angiogenesis in human placental vessels ex\u00a0vivo and endothelial cells in\u00a0vitro.","volume":"151","author":"Ramaesh","year":"2010","journal-title":"Endocrinology"},{"key":"2025102820104440400_R37","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1111\/J.1365-2826.2009.01905.X","article-title":"Studies of the localisation of kisspeptin within the pituitary of the rhesus monkey (Macaca mulatta) and the effect of kisspeptin on the release of non-gonadotropic pituitary hormones.","volume":"21","author":"Ramaswamy","year":"2009","journal-title":"J. Neuroendocrinol."},{"key":"2025102820104440400_R38","doi-asserted-by":"publisher","first-page":"2485","DOI":"10.1080\/07391102.2016.1222970","article-title":"In silico analysis and expression studies of kisspeptin gene in C. catla.","volume":"35","author":"Rather","year":"2016","journal-title":"J. Biomol. Struct. Dyn."},{"key":"2025102820104440400_R39","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1111\/J.1365-2826.2008.01653.X","article-title":"KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in\u00a0vivo by oestradiol and gonadotrophin-releasing hormone.","volume":"20","author":"Richard","year":"2008","journal-title":"J. Neuroendocrinol."},{"key":"2025102820104440400_R40","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/J.YFRNE.2007.07.002","article-title":"New frontiers in kisspeptin\/GPR54 physiology as fundamental gatekeepers of reproductive function.","volume":"29","author":"Roa","year":"2008","journal-title":"Front. Neuroendocrinol."},{"key":"2025102820104440400_R41","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/J.CBPB.2015.10.008","article-title":"Molecular characterization of two kiss genes and their expression in rohu (Labeorohita) during annual reproductive cycle.","volume":"191","author":"Saha","year":"2016","journal-title":"Comp. Biochem. Physiol. B"},{"key":"2025102820104440400_R42","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/J.YGCEN.2014.09.019","article-title":"Expression changes of mRNAs encoding kisspeptins and their receptors and gonadotropin-releasing hormones during early development and gonadal sex differentiation periods in the brain of chub mackerel (Scomber japonicus).","volume":"222","author":"Selvaraj","year":"2015","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R43","doi-asserted-by":"publisher","first-page":"1527","DOI":"10.1210\/EN.2010-0948","article-title":"Organization of two independent kisspeptin systems derived from evolutionary-ancient kiss genes in the brain of zebrafish.","volume":"152","author":"Servili","year":"2011","journal-title":"Endocrinology"},{"key":"2025102820104440400_R44","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/J.CBPB.2016.11.010","article-title":"Molecular cloning, characterization and expression profile of kisspeptin1 and kisspeptin1 receptor at brain\u2013pituitary\u2013gonad (BPG) axis of golden mahseer, Tor putitora (Hamilton, 1822) during gonadal development.","volume":"205","author":"Shahi","year":"2017","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"2025102820104440400_R45","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1007\/S10695-016-0199-1","article-title":"Identification and characterization of kiss2 and kissr2 homologs in Paralichthysolivaceus.","volume":"42","author":"Song","year":"2016","journal-title":"Fish Physiol. Biochem."},{"key":"2025102820104440400_R46","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1007\/S10695-016-0328-X","article-title":"Characterization of kiss2 and kissr2 genes and the regulation of kisspeptin on the HPG axis in Cynoglossussemilaevis.","volume":"43","author":"Song","year":"2017","journal-title":"Fish Physiol. Biochem."},{"key":"2025102820104440400_R47","first-page":"5399","article-title":"Identification and characterization of mouse metastasis-suppressor KiSS1 and its G-protein-coupled receptor.","volume":"62","author":"Stafford","year":"2002","journal-title":"Cancer Res."},{"key":"2025102820104440400_R48","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/J.YGCEN.2009.05.004","article-title":"Control of puberty in farmed fish.","volume":"165","author":"Taranger","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R49","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/J.YGCEN.2011.11.015","article-title":"Comparative insights of the kisspeptin\/kisspeptin receptor system: lessons from non-mammalian vertebrates.","volume":"175","author":"Tena-Sempere","year":"2012","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R50","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1095\/BIOLREPROD.109.079863","article-title":"Molecular characterization and estrogen regulation of hypothalamic KISS1 gene in the pig.","volume":"82","author":"Tomikawa","year":"2010","journal-title":"Biol. Reprod."},{"key":"2025102820104440400_R51","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1111\/J.1749-6632.2010.05508.X","article-title":"Molecular coevolution of kisspeptins and their receptors from fish to mammals.","volume":"1200","author":"Um","year":"2010","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2025102820104440400_R52","doi-asserted-by":"publisher","first-page":"2050","DOI":"10.1360\/03WC0076","article-title":"Complete mitochondrial DNA sequence of Chinese alligator, Alligator sinensis, and phylogeny of crocodiles.","volume":"48","author":"Wu","year":"2003","journal-title":"Chin. Sci. Bull."},{"key":"2025102820104440400_R53","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1111\/1749-4877.12223","article-title":"Seasonal differential expression of KiSS-1\/GPR54 in the striped hamsters (Cricetulusbarabensis) among different tissues.","volume":"12","author":"Xu","year":"2016","journal-title":"Integr. Zool."},{"key":"2025102820104440400_R54","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/J.YGCEN.2009.06.001","article-title":"Goldfish kisspeptin: molecular cloning, tissue distribution of transcript expression, and stimulatory effects on prolactin, growth hormone and luteinizing hormone secretion and gene expression via direct actions at the pituitary level.","volume":"165","author":"Yang","year":"2009","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102820104440400_R55","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1007\/S11033-016-4014-Y","article-title":"Molecular identification of Kiss\/GPR54 and function analysis with mRNA expression profiles exposure to 17\u03b1-ethinylestradiol in rare minnow Gobiocyprisrarus.","volume":"43","author":"Yang","year":"2016","journal-title":"Mol. Biol. Rep."},{"key":"2025102820104440400_R56","doi-asserted-by":"publisher","first-page":"2582","DOI":"10.1210\/EN.2015-1131","article-title":"Conditional viral tract tracing delineates the projections of the distinct kisspeptin neuron populations to gonadotropin-releasing hormone (GnRH) neurons in the mouse.","volume":"156","author":"Yip","year":"2015","journal-title":"Endocrinology"},{"key":"2025102820104440400_R57","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/J.CBPB.2016.05.001","article-title":"Molecular cloning, characterization, tissue distribution and mRNA expression changes during the hibernation and reproductive periods of estrogen receptor alpha (esr1) in Chinese alligator, Alligatorsinensis.","volume":"200","author":"Zhang","year":"2016","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"2025102820104440400_R58","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/J.GENE.2017.04.028","article-title":"Molecular cloning of esr2 and gene expression analysis of esr1 and esr2 in the pituitary gland of the Chinese alligator (Alligator sinensis) during female reproductive cycle.","volume":"623","author":"Zhang","year":"2017","journal-title":"Gene"},{"key":"2025102820104440400_R59","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/J.GENE.2018.06.033","article-title":"Molecular cloning of androgen receptor and gene expression of sex steroid hormone receptors in the brain of newborn Chinese alligator (Alligator sinensis).","volume":"674","author":"Zhang","year":"2018","journal-title":"Gene"},{"key":"2025102820104440400_R60","doi-asserted-by":"publisher","first-page":"1874","DOI":"10.1210\/EN.2013-1894","article-title":"The medio-basal hypothalamus as a dynamic and plastic reproduction-related kisspeptin\u2013GnRH\u2013pituitary center in fish.","volume":"155","author":"Zmora","year":"2014","journal-title":"Endocrinology"},{"key":"2025102820104440400_R61","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1016\/J.YGCEN.2009.04.017","article-title":"Neuroendocrinology of reproduction in teleost fish.","volume":"165","author":"Zohar","year":"2010","journal-title":"Gen. Comp. Endocrinol."}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/32\/8\/792\/316358\/rd19332.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/32\/8\/792\/316358\/rd19332.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:11:17Z","timestamp":1761696677000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/32\/8\/792\/50627\/Cloning-characterisation-and-expression-profile-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":61,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,5,11]]}},"URL":"https:\/\/doi.org\/10.1071\/rd19332","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"type":"print","value":"1031-3613"},{"type":"electronic","value":"1448-5990"}],"subject":[],"published":{"date-parts":[[2020,4,20]]}}}