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There was a significant reduction in intratesticular testosterone (ITT) concentrations and testicular steroidogenic enzyme mRNA expression and a significant increase in germ cell apoptosis in DBP-treated compared with control quails at all time points. Maximum apoptosis was detected 6 h after treatment and the maximum reduction in testosterone concentrations was at 3 h. To investigate whether DBP suppressed testicular steroidogenesis by affecting the hypothalamic\u2013pituitary\u2013testicular axis, we analysed pituitary LH subunit \u03b2 (Lhb) mRNA expression and serum LH concentrations. At all time points, pituitary Lhb expression and serum LH concentrations were significantly decreased following DBP treatment. The present observations suggest the possibility that DBP blocked LH secretion from the hypothalamus and\/or pituitary, thereby decreasing LH stimulation of Leydig cells and reducing ITT concentrations. DBP-induced decreases in ITT concentrations may cause changes to the physical structure of Sertoli cells, which, in turn, may induce germ cell apoptosis.<\/jats:p>","DOI":"10.1071\/rd20150","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T00:57:36Z","timestamp":1614301056000},"page":"319-327","source":"Crossref","is-referenced-by-count":12,"title":["Di-n-butyl phthalate diminishes testicular steroidogenesis by blocking the hypothalamic\u2013pituitary\u2013testicular axis: relationship with germ cell apoptosis in Japanese quail"],"prefix":"10.1071","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2657-9121","authenticated-orcid":true,"given":"Mohammad Shah","family":"Alam","sequence":"first","affiliation":[{"name":"ADepartment of Anatomy and Histology, Faculty of Veterinary Medicine and Animal Sciences, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh."},{"name":"CDepartment of Veterinary Anatomy, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan."},{"name":"DCorresponding author. 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