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After storage (0, 24, 48 and 72 h), 1 \u03bcM MLT in semen markedly improved sperm quality, as reflected by better plasma membrane integrity, the relative steady level of reactive oxygen species (ROS) and slower rate of decrease in mitochondrial membrane potential. Activated spermatozoa in semen with 1 \u03bcM MLT had higher kinematic performance (i.e. percentage of motile and progressive spermatozoa and the beat cross frequency; P &amp;lt; 0.05) and longer duration of sperm motility (P &amp;lt; 0.05) compared with spermatozoa in semen withother MLT concentrations. Furthermore, 1 \u03bcM MLT maintained higher ATP concentrations in spermatozoa during semen storage and significantly improved the fertilising capacity of spermatozoa after 72 h semen storage compared with the other MLT concentrations. To expand wild resources of O. macrolepis, 1 \u03bcM MLT can be used as a semen additive to maintain better sperm function and enhance sperm fertilising capacity in artificial insemination (AI).<\/jats:p>","DOI":"10.1071\/rd20153","type":"journal-article","created":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T22:46:59Z","timestamp":1601938019000},"page":"1212-1222","source":"Crossref","is-referenced-by-count":8,"title":["Melatonin regulates ATP content and fertilising capacity of Onychostoma macrolepis spermatozoa by inhibiting ROS accumulation during semen storage in\u2009vitro"],"prefix":"10.1071","volume":"32","author":[{"given":"Jinmeng","family":"Yang","sequence":"first","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daqian","family":"Dong","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofan","family":"Peng","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingfang","family":"Sun","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenhao","family":"Yang","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Gao","sequence":"additional","affiliation":[{"name":"BNutrigenomics and Growth Biology Laboratory, Department of Animal Sciences, Washington State University, Pullman, WA 99163, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Ji","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4363-8456","authenticated-orcid":true,"given":"Wuzi","family":"Dong","sequence":"additional","affiliation":[{"name":"ACollege of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China."},{"name":"CCorresponding author. 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