{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T08:27:56Z","timestamp":1782376076209,"version":"3.54.5"},"reference-count":17,"publisher":"CSIRO Publishing","issue":"6","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":1544,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,9]]},"abstract":"<jats:p>Urethral catheterisation after medetomidine administration is the method of choice for semen collection in cats, but it yields variable results. This study tested whether scrotal manual stimulation can improve urethral sperm collection in domestic cats. The study was performed on 20 male cats, from which two urethral semen samples were collected, one before and one after 2 min of transscrotal finger massage of the testes and epididymides. Both sperm samples were assessed for total sperm count and motility using computer-aided sperm analysis, viability and morphology (eosin\u2013nigrosin staining). The transscrotal manual stimulation allowed a significantly higher number of spermatozoa to be obtained (P = 0.0015). Viability was similar before and after the stimulation (median 92% and 90.5%), whereas the number of motile (median 60% and 70%) and morphologically normal (median 17% and 30.5%) spermatozoa was higher in the second sample (P = 0.03 and P = 0.002 respectively), which confirms that transscrotal massage induced the expulsion of a fresh pool of spermatozoa into the urethra. Transscrotal stimulation of the testes and epididymides significantly improves urethral semen collection in domestic cats and can be easily introduced into clinical practice.<\/jats:p>","DOI":"10.1071\/rd21010","type":"journal-article","created":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T18:17:46Z","timestamp":1614881866000},"page":"437-440","source":"Crossref","is-referenced-by-count":11,"title":["Transscrotal stimulation of the testes and epididymides improves urethral sperm collection in domestic cats"],"prefix":"10.1071","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5708-8141","authenticated-orcid":true,"given":"S.","family":"Prochowska","sequence":"first","affiliation":[{"name":"ADepartment of Reproduction and Clinic of Farm Animals, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland."},{"name":"BCorresponding author. Email: sylwia.prochowska@upwr.edu.pl"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Ni\u017ca\u0144ski","sequence":"additional","affiliation":[{"name":"ADepartment of Reproduction and Clinic of Farm Animals, Wroc\u0142aw University of Environmental and Life Sciences, 50-366 Wroc\u0142aw, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2021,3,5]]},"reference":[{"key":"2025102820153602400_R1","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1093\/HUMREP\/DEX101","article-title":"Improved sperm kinematics in semen samples collected after 2 h versus 4\u20137 days of ejaculation abstinence.","volume":"32","author":"Alipour","year":"2017","journal-title":"Hum. 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