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Compared to the control, deterioration in spermatological parameters and significant increases in lipid peroxidation and global DNA methylation levels were observed in groups 3 and 4. When compared with the control, significant downregulation in the levels of miR-485 of group 2, miR-29a of group 3 and let-7a, miR-485 and miR-29a of group 4, and significant upregulation in the levels of miR-107 of group 3 and miR-127 of groups 3 and 4 were detected. In comparison to the control, significant upregulation in the levels of CatSper1, CatSper2, CatSper3, CatSper4, ANO1 and TRPM3 of group 2, CatSper4, ANO1 and TRPM3 of group 3 and KCNJ11 of group 4, and significant downregulation in the CatSper 3 level of group 4 were determined. As a result, the semen freeze\u2013thawing process causes motility and morphological disorders in rams. This may be due to molecular changes associated with lipid peroxidation in spermatozoa.<\/jats:p>","DOI":"10.1071\/rd21091","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T05:54:22Z","timestamp":1632894862000},"page":"747-759","source":"Crossref","is-referenced-by-count":27,"title":["Effect of freeze\u2013thawing process on lipid peroxidation, miRNAs, ion channels, apoptosis and global DNA methylation in ram spermatozoa"],"prefix":"10.1071","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5250-1478","authenticated-orcid":true,"given":"\u0130brahim Halil","family":"G\u00fcng\u00f6r","sequence":"first","affiliation":[{"name":"AFaculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, F\u0131rat University, Elaz\u0131\u011f, 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