{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:25:49Z","timestamp":1787005549403,"version":"build-2736575974"},"reference-count":58,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":1209,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,9]]},"abstract":"<jats:p>The aim of this study was to investigate: (1) the ability of granulosa cells to produce amphiregulin (AREG), kisspeptin (KISS) and FSH receptor (FSHR); (2) the role of AREG and KISS in the control of ovarian functions; (3) the effect of FSH and KISS on AREG; and (4) the ability of KISS to affect FSHR and to modify FSH action on AREG output by human ovarian granulosa cells. We examined: (1) time-dependent accumulation of AREG; (2) effects of AREG (0, 1, 10, 100\u00a0ng\/mL) and KISS (0, 1, 10, 100\u00a0ng\/mL) on granulosa cell functions; and (3) the effects of KISS (0, 1, 10, 100\u00a0ng\/mL), FSH (0, 1, 10, 100\u00a0ng\/mL), and their combinations on AREG release. Viability, markers of proliferation [accumulation of\u00a0proliferating cell nuclear antigen (PCNA) cyclin B1 and sodium 3\u2032-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy6-nitro)benzene sulfonic acid hydrate (XTT formazan)] and apoptosis (accumulation of bax, caspase 3 and terminal deoxynucleotidyl transferase dUTP nick-end labelling), accumulation of KISS, FSHR and steroid hormones, and AREG release were analysed by Trypan blue exclusion test, quantitative immunocytochemistry, XTT, terminal deoxynucleotidyl transferase dUTP nick-end labelling assays and enzyme-linked immunosorbent assay. AREG promoted cell viability, proliferation and steroid hormone output, and inhibited apoptosis. KISS (1 and 10\u00a0ng\/mL) stimulated viability, proliferation, steroid hormone release and occurrence of FSHR and suppressed apoptosis and AREG output; KISS (100\u00a0ng\/mL) had the opposite effect. FSH stimulated AREG release, whilst addition of KISS reversed this FSH effect. FSH mimicked and promoted the inhibitory effect of KISS on AREG release. These results suggest an intra-ovarian production and a functional interrelationship between AREG, KISS, FSH and FSHR in direct regulation of basic ovarian cell functions.<\/jats:p>","DOI":"10.1071\/rd21230","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T02:01:36Z","timestamp":1643853696000},"page":"362-377","source":"Crossref","is-referenced-by-count":16,"title":["Interrelationships between amphiregulin, kisspeptin, FSH and FSH receptor in promotion of human ovarian cell functions"],"prefix":"10.1071","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5063-6344","authenticated-orcid":true,"given":"Zuzana","family":"Fabov\u00e1","sequence":"first","affiliation":[{"name":"ADepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A. Hlinku 1, 94974 Nitra, Slovakia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbora","family":"Loncov\u00e1","sequence":"additional","affiliation":[{"name":"ADepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A. Hlinku 1, 94974 Nitra, Slovakia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milo\u0161","family":"Mlyn\u010dek","sequence":"additional","affiliation":[{"name":"ADepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A. Hlinku 1, 94974 Nitra, Slovakia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-3512","authenticated-orcid":true,"given":"Alexander V.","family":"Sirotkin","sequence":"additional","affiliation":[{"name":"ADepartment of Zoology and Anthropology, Constantine the Philosopher University, Tr. A. Hlinku 1, 94974 Nitra, Slovakia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2022,2,3]]},"reference":[{"issue":"13","key":"2025102820260689800_R1","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1016\/j.steroids.2012.07.011","article-title":"Progesterone production requires activation of caspase-3 in preovulatory granulosa cells in a serum starvation model.","volume":"77","author":"An","year":"2012","journal-title":"Steroids"},{"issue":"11","key":"2025102820260689800_R2","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1002\/mrd.22876","article-title":"Kisspeptin regulates ovarian steroidogenesis during delayed embryonic development in the fruit bat, Cynopterus sphinx.","volume":"84","author":"Anuradha","year":"2017","journal-title":"Molecular Reproduction & Development"},{"key":"2025102820260689800_R3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.theriogenology.2018.04.006","article-title":"Presence and function of kisspeptin\/KISS1R system in swine ovarian follicles.","volume":"115","author":"Basini","year":"2018","journal-title":"Theriogenology"},{"key":"2025102820260689800_R4","doi-asserted-by":"publisher","first-page":"106466","DOI":"10.1016\/j.domaniend.2020.106466","article-title":"The kisspeptin system in domestic animals: what we know and what we still need to understand of its role in reproduction.","volume":"73","author":"Beltramo","year":"2020","journal-title":"Domestic Animal Endocrinology"},{"key":"2025102820260689800_R5","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/S1387-2656(05)11004-7","article-title":"Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction.","volume":"11","author":"Berridge","year":"2005","journal-title":"Biotechnology Annual Review"},{"issue":"4","key":"2025102820260689800_R6","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/j.fertnstert.2020.05.006","article-title":"Female infertility is associated with an altered expression of the neurokinin B\/neurokinin B receptor and kisspeptin\/kisspeptin receptor systems in ovarian granulosa and cumulus cells.","volume":"114","author":"Blasco","year":"2020","journal-title":"Fertility and Sterility"},{"issue":"1","key":"2025102820260689800_R7","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1186\/s12958-019-0511-x","article-title":"Reproductive functions of Kisspeptin\/KISS1R systems in the periphery.","volume":"17","author":"Cao","year":"2019","journal-title":"Reproductive Biology and Endocrinology"},{"issue":"11","key":"2025102820260689800_R8","doi-asserted-by":"publisher","first-page":"5258","DOI":"10.1210\/en.2007-0554","article-title":"Kisspeptin synchronizes preovulatory surges in cyclical ewes and causes ovulation in seasonally acyclic ewes.","volume":"148","author":"Caraty","year":"2007","journal-title":"Endocrinology"},{"issue":"2","key":"2025102820260689800_R9","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.domaniend.2012.03.002","article-title":"Kisspeptins and the reproductive axis: potential applications to manage reproduction in farm animals.","volume":"43","author":"Caraty","year":"2012","journal-title":"Domestic Animal Endocrinology"},{"issue":"1","key":"2025102820260689800_R10","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.peptides.2008.07.025","article-title":"Intracellular signaling pathways activated by kisspeptins through GPR54: do multiple signals underlie function diversity?","volume":"30","author":"Casta\u00f1o","year":"2009","journal-title":"Peptides"},{"issue":"10","key":"2025102820260689800_R11","doi-asserted-by":"publisher","first-page":"4852","DOI":"10.1210\/en.2006-0117","article-title":"Expression of KiSS-1 in rat ovary: putative local regulator of ovulation?","volume":"147","author":"Castellano","year":"2006","journal-title":"Endocrinology"},{"key":"2025102820260689800_R12"},{"key":"2025102820260689800_R13","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1111\/rda.12841","article-title":"The localization of kisspeptin and kisspeptin receptor in the canine ovary during different stages of the reproductive cycle.","volume":"52","author":"Cielesh","year":"2017","journal-title":"Reproduction in Domestic Animals"},{"issue":"4","key":"2025102820260689800_R14","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1210\/me.2005-0185","article-title":"Role of the epidermal growth factor network in ovarian follicles.","volume":"20","author":"Conti","year":"2006","journal-title":"Molecular Endocrinology"},{"issue":"8","key":"2025102820260689800_R15","doi-asserted-by":"publisher","first-page":"653","DOI":"10.7150\/jca.7626","article-title":"Kisspeptin\/KISS1R system in breast cancer.","volume":"4","author":"Cvetkovi\u0107","year":"2013","journal-title":"Journal of Cancer"},{"issue":"3","key":"2025102820260689800_R16","doi-asserted-by":"publisher","first-page":"R131","DOI":"10.1530\/JME-17-0308","article-title":"Molecular regulation of follicle-stimulating hormone synthesis, secretion and action.","volume":"60","author":"Das","year":"2018","journal-title":"Journal of Molecular Endocrinology"},{"issue":"6","key":"2025102820260689800_R17","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1093\/biolre\/ioaa135","article-title":"Peripheral action of kisspeptin at reproductive tissues \u2013 role in ovarian function and embryo implantation and relevance to assisted reproductive technology in livestock: a review.","volume":"103","author":"D\u2019Occhio","year":"2020","journal-title":"Biology of Reproduction"},{"issue":"47","key":"2025102820260689800_R18","doi-asserted-by":"publisher","first-page":"16778","DOI":"10.1073\/pnas.1414648111","article-title":"Follicle-stimulating hormone regulates expression and activity of epidermal growth factor receptor in the murine ovarian follicle.","volume":"111","author":"El-Hayek","year":"2014","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"2025102820260689800_R19","doi-asserted-by":"publisher","first-page":"106520","DOI":"10.1016\/j.domaniend.2020.106520","article-title":"Interrelationships between kisspeptin and FSH in control of porcine ovarian cell functions.","volume":"74","author":"Fabov\u00e1","year":"2021","journal-title":"Domestic Animal Endocrinology"},{"key":"2025102820260689800_R20","doi-asserted-by":"publisher","first-page":"24917","DOI":"10.1038\/srep24917","article-title":"Amphiregulin mediates hCG-induced StAR expression and progesterone production in human granulosa cells.","volume":"6","author":"Fang","year":"2016","journal-title":"Scientific Reports"},{"issue":"3","key":"2025102820260689800_R21","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1530\/JOE-15-0429","article-title":"Kisspeptin is involved in ovarian follicular development during aging in rats.","volume":"228","author":"Fernandois","year":"2016","journal-title":"Journal of Endocrinology"},{"issue":"2","key":"2025102820260689800_R22","doi-asserted-by":"publisher","first-page":"829","DOI":"10.1016\/j.bbrc.2004.09.129","article-title":"EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins\/cAMP in human ovarian follicular cells.","volume":"324","author":"Freimann","year":"2004","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"12","key":"2025102820260689800_R23","doi-asserted-by":"publisher","first-page":"2736","DOI":"10.1093\/humrep\/deu247","article-title":"Expression of neurokinin B\/NK3 receptor and kisspeptin\/KISS1 receptor in human granulosa cells.","volume":"29","author":"Garc\u00eda-Ortega","year":"2014","journal-title":"Human Reproduction"},{"issue":"6","key":"2025102820260689800_R24","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1095\/biolreprod.116.139881","article-title":"Expression of tachykinins and tachykinin receptors and interaction with kisspeptin in human granulosa and cumulus cells.","volume":"94","author":"Garc\u00eda-Ortega","year":"2016","journal-title":"Biology of Reproduction"},{"key":"2025102820260689800_R25","doi-asserted-by":"publisher","first-page":"804","DOI":"10.1038\/sj.cdd.4400714","article-title":"Bcl-2 and Bax mammalian regulators of apoptosis are functional in Drosophila.","volume":"7","author":"Gaumer","year":"2000","journal-title":"Cell Death & Differentiation"},{"issue":"3","key":"2025102820260689800_R26","doi-asserted-by":"publisher","first-page":"E520","DOI":"10.1152\/ajpendo.90895.2008","article-title":"KiSS-1 in the mammalian ovary: distribution of kisspeptin in human and marmoset and alterations in KiSS-1 mRNA levels in a rat model of ovulatory dysfunction.","volume":"296","author":"Gayt\u00e1n","year":"2009","journal-title":"American Journal of Physiology\u2013Endocrinology and Metabolism"},{"issue":"9","key":"2025102820260689800_R27","doi-asserted-by":"publisher","first-page":"4073","DOI":"10.1210\/en.2004-0431","article-title":"A role for kisspeptins in the regulation of gonadotropin secretion in the mouse.","volume":"145","author":"Gottsch","year":"2004","journal-title":"Endocrinology"},{"issue":"1","key":"2025102820260689800_R28","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1055\/s-0028-1108010","article-title":"Epidermal growth factor-like growth factors in the follicular fluid: role in oocyte development and maturation.","volume":"27","author":"Hsieh","year":"2009","journal-title":"Seminars in Reproductive Medicine"},{"key":"2025102820260689800_R29","doi-asserted-by":"publisher","first-page":"365","DOI":"10.3389\/fendo.2017.00365","article-title":"Kisspeptin\/kisspeptin receptor system in the ovary.","volume":"8","author":"Hu","year":"2018","journal-title":"Frontiers in Endocrinology"},{"issue":"1","key":"2025102820260689800_R30","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1095\/biolreprod.115.127902","article-title":"Augmentation of metastin\/kisspeptin mRNA expression by the proestrous luteinizing hormone surge in granulosa cells of rats: implications for luteinization1.","volume":"93","author":"Laoharatchatathanin","year":"2015","journal-title":"Biology of Reproduction"},{"key":"2025102820260689800_R31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anireprosci.2017.07.008","article-title":"Effect of kisspeptin on the proliferation and apoptosis of bovine granulosa cells.","volume":"185","author":"Liu","year":"2017","journal-title":"Animal Reproduction Science"},{"issue":"2","key":"2025102820260689800_R32","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.bbrc.2004.05.185","article-title":"Peripheral administration of metastin induces marked gonadotropin release and ovulation in the rat.","volume":"320","author":"Matsui","year":"2004","journal-title":"Biochemical and Biophysical Research Communications"},{"issue":"23","key":"2025102820260689800_R33","doi-asserted-by":"publisher","first-page":"9090","DOI":"10.3390\/ijms21239090","article-title":"Do TUNEL and other apoptosis assays detect cell death in preclinical studies?","volume":"21","author":"Mirzayans","year":"2020","journal-title":"International Journal of Molecular Sciences"},{"key":"2025102820260689800_R34","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1016\/B978-0-12-407695-2.00007-X","article-title":"Role of cyclin B1 levels in DNA damage and DNA damage-induced senescence.","volume":"305","author":"Nakayama","year":"2013","journal-title":"International Review of Cell and Molecular Biology"},{"issue":"1","key":"2025102820260689800_R35","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1210\/en.2004-0836","article-title":"Characterization of the potent luteinizing hormone-releasing activity of KiSS-1 peptide, the natural ligand of GPR54.","volume":"146","author":"Navarro","year":"2005","journal-title":"Endocrinology"},{"key":"2025102820260689800_R36"},{"issue":"2","key":"2025102820260689800_R37","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1093\/humrep\/dex357","article-title":"The direct and indirect effects of kisspeptin-54 on granulosa lutein cell function.","volume":"33","author":"Owens","year":"2018","journal-title":"Human Reproduction"},{"issue":"5658","key":"2025102820260689800_R38","doi-asserted-by":"publisher","first-page":"682","DOI":"10.1126\/science.1092463","article-title":"EGF-like growth factors as mediators of LH action in the ovulatory follicle.","volume":"303","author":"Park","year":"2004","journal-title":"Science"},{"issue":"8","key":"2025102820260689800_R39","doi-asserted-by":"publisher","first-page":"2430","DOI":"10.1093\/humrep\/zdes158","article-title":"Amphiregulin promotes the maturation of oocytes isolated from the small antral follicles of the rhesus macaque.","volume":"27","author":"Peluffo","year":"2012","journal-title":"Human Reproduction"},{"issue":"3","key":"2025102820260689800_R40","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1111\/febs.14186","article-title":"Bax, Bak and beyond \u2013 mitochondrial performance in apoptosis.","volume":"285","author":"Pe\u00f1a-Blanco","year":"2018","journal-title":"The FEBS Journal"},{"issue":"5","key":"2025102820260689800_R41","doi-asserted-by":"publisher","first-page":"1436","DOI":"10.1016\/j.fertnstert.2012.12.008","article-title":"Kisspeptin stimulates progesterone secretion via the Erk1\/2 mitogen-activated protein kinase signaling pathway in rat luteal cells.","volume":"99","author":"Peng","year":"2013","journal-title":"Fertility and Sterility"},{"issue":"1","key":"2025102820260689800_R42","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1095\/biolreprod.112.103002","article-title":"EGF-like ligands mediate progesterone\u2019s anti-apoptotic action on macaque granulosa cells.","volume":"88","author":"Puttabyatappa","year":"2013","journal-title":"Biology of Reproduction"},{"issue":"5","key":"2025102820260689800_R43","doi-asserted-by":"publisher","first-page":"618","DOI":"10.6002\/ect.2019.0113","article-title":"Effects of ovarian transplant on anti-m\u00fcllerian hormone, kisspeptin-1, and kisspeptin-1 receptor levels in rats.","volume":"18","author":"Risvanli","year":"2020","journal-title":"Experimental and Clinical Transplantation"},{"issue":"3","key":"2025102820260689800_R44","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/j.fertnstert.2020.06.038","article-title":"Role of kisspeptins in the control of the hypothalamic\u2013pituitary\u2013ovarian axis: old dogmas and new challenges.","volume":"114","author":"Ruohonen","year":"2020","journal-title":"Fertility and Sterility"},{"issue":"5","key":"2025102820260689800_R45","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1071\/RD11118","article-title":"Paradoxical effects of kisspeptin: it enhances oocyte in vitro maturation but has an adverse impact on hatched blastocysts during in vitro culture.","volume":"24","author":"Saadeldin","year":"2012","journal-title":"Reproduction, Fertility and Development"},{"key":"2025102820260689800_R46","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.ygcen.2012.10.012","article-title":"Human chorionic gonadotropin-dependent up-regulation of epiregulin and amphiregulin in equine and bovine follicles during the ovulatory process.","volume":"180","author":"Sayasith","year":"2013","journal-title":"General and Comparative Endocrinology"},{"issue":"1","key":"2025102820260689800_R47","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1677\/jme.0.0330281","article-title":"Expression of epiregulin and amphiregulin in the rat ovary.","volume":"33","author":"Sekiguchi","year":"2004","journal-title":"Journal of Molecular Endocrinology"},{"issue":"4","key":"2025102820260689800_R48","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1007\/s12522-016-0236-x","article-title":"Roles of epidermal growth factor (EGF)-like factor in the ovulation process.","volume":"15","author":"Shimada","year":"2016","journal-title":"Reproductive Medicine and Biology"},{"issue":"2","key":"2025102820260689800_R49","doi-asserted-by":"publisher","first-page":"52","DOI":"10.3390\/genes8020052","article-title":"Control of genome integrity by RFC complexes; conductors of PCNA loading onto and unloading from chromatin during DNA replication.","volume":"8","author":"Shiomi","year":"2017","journal-title":"Genes"},{"key":"2025102820260689800_R50"},{"key":"2025102820260689800_R51","doi-asserted-by":"publisher","first-page":"172560","DOI":"10.1016\/j.ejphar.2019.172560","article-title":"Quercetin directly inhibits basal ovarian cell functions and their response to the stimulatory action of FSH.","volume":"860","author":"Sirotkin","year":"2019","journal-title":"European Journal of Pharmacology"},{"issue":"4","key":"2025102820260689800_R52","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1093\/humupd\/dmu009","article-title":"The kisspeptin\u2013GnRH pathway in human reproductive health and disease.","volume":"20","author":"Skorupskaite","year":"2014","journal-title":"Human Reproduction Update"},{"key":"2025102820260689800_R53","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.critrevonc.2019.07.015","article-title":"The role of kisspeptin system in cancer biology.","volume":"142","author":"Stathaki","year":"2019","journal-title":"Critical Reviews in Oncology\/Hematology"},{"key":"2025102820260689800_R54","doi-asserted-by":"publisher","first-page":"A.3B.1","DOI":"10.1002\/0471142735.ima03bs21","article-title":"Trypan blue exclusion test of cell viability.","volume":"Suppl_21","author":"Strober","year":"2001","journal-title":"Current Protocols in Immunology"},{"issue":"5","key":"2025102820260689800_R55","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1007\/s40618-019-01160-0","article-title":"Kisspeptin and neurokinin B analogs use in gynecological endocrinology: where do we stand?","volume":"43","author":"Szeliga","year":"2020","journal-title":"Journal of Endocrinological Investigation"},{"key":"2025102820260689800_R56","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1007\/978-1-4614-6199-9_12","article-title":"Kisspeptin and puberty in mammals.","volume":"784","author":"Terasawa","year":"2013","journal-title":"Advances in Experimental Medicine and Biology"},{"issue":"10","key":"2025102820260689800_R57","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1016\/j.peptides.2011.09.001","article-title":"Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory (F1\u2013F3) follicles.","volume":"32","author":"Xiao","year":"2011","journal-title":"Peptides"},{"key":"2025102820260689800_R58","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1186\/1477-7827-12-127","article-title":"High-fat diet decreases the expression of Kiss1 mRNA and kisspeptin in the ovary, and increases ovulatory dysfunction in postpubertal female rats.","volume":"12","author":"Zhou","year":"2014","journal-title":"Reproductive Biology and Endocrinology"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/34\/3\/362\/319406\/rd21230.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/34\/3\/362\/319406\/rd21230.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:26:15Z","timestamp":1761697575000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/34\/3\/362\/50954\/Interrelationships-between-amphiregulin-kisspeptin"}},"subtitle":[],"editor":[{"given":"Jennifer","family":"Juengel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,2,3]]},"references-count":58,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,2,9]]}},"URL":"https:\/\/doi.org\/10.1071\/rd21230","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,3]]}}}