{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T22:33:00Z","timestamp":1747175580806,"version":"3.40.5"},"reference-count":31,"publisher":"CSIRO Publishing","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod. Fertil. Dev."],"accepted":{"date-parts":[[2023,3,7]]},"abstract":"<jats:p>Context   Sperm storage is a complex and highly coordinated process that is regulated by a variety of factors. The BCL 2 protein family plays a key role in regulating apoptosis, and determines sperm survival.     Aims   The objective of this study was to explore the correlation between sperm storage and the BCL 2 protein family in the oviduct of Mauremys reevesii.     Methods   Hematoxylin-eosin (HE) staining, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) techniques were used to investigate three parts of the reproductive tract (isthmus, uterus and vagina) of mated and unmated female M. reevesii.     Key results   Hematoxylin-eosin staining revealed many sperm stored in the oviduct. IHC showed positive immunostaining for the BCL 2 and BAX proteins in epithelial ciliated and glandular cells. RT-qPCR indicated that the mRNA expressions of anti-apoptotic genes (BCL 2, MCL 1, BCL-W, BCL-XL) and the androgen receptor (AR) were significantly higher in mated turtles than unmated turtles. However, the expression of pro-apoptotic genes (BAX, BAD, BID and CASPASE 3) showed the opposite relationship.     Conclusions   These results suggest that sperm entering the oviduct can promote the synthesis of anti-apoptotic genes to protect themselves from various degradation factors.     Implications   These findings will help researchers understand the mechanisms of sperm storage.<\/jats:p>","DOI":"10.1071\/rd22205","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T21:59:46Z","timestamp":1679608786000},"page":"406-415","source":"Crossref","is-referenced-by-count":0,"title":["Oviductal sperm storage in the Chinese pond turtle, Mauremys reevesii, depends on androgen-based promotion of the BCL 2 anti-apoptotic 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