{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:17:01Z","timestamp":1782188221046,"version":"3.54.5"},"reference-count":67,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":505,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871203"],"award-info":[{"award-number":["81871203"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,25]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>The human TSPY1 (testis-specific protein, Y-linked 1) gene is critical for spermatogenesis and male fertility. However, there have been difficulties with studying the mechanism underlying its function, partly due to the presence of the Tspy1 pseudogene in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>TSPYL5 (TSPY-like 5), an autosomal homologous gene of TSPY1 showing a similar expression pattern in both human and mouse testes, is also speculated to play a role in male spermatogenesis. It is beneficial to understand the role of TSPY1 in spermatogenesis by investigating Tspyl5 functions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Tspyl5-knockout mice were generated to investigate the effect of TSPYL5 knockout on spermatogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Tspyl5 deficiency caused a decline in fertility and decreased the numbers of spermatogonia and spermatozoa in aged male mice. Trancriptomic detection of spermatogonia derived from aged Tspyl5-knockout mice revealed that the Pcna-mediated DNA replication pathway was downregulated. Furthermore, Tspyl5 was proven to facilitate spermatogonia proliferation and upregulate Pcna expression by promoting the ubiquitination-degradation of the TRP53 protein.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings suggest that Tspyl5 is a positive regulator for the maintenance of the spermatogonia pool by enhancing Pcna-mediated DNA replication.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>This observation provides an important clue for further investigation of the spermatogenesis-related function of TSPY1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd23042","type":"journal-article","created":{"date-parts":[[2024,1,7]],"date-time":"2024-01-07T18:07:31Z","timestamp":1704650851000},"source":"Crossref","is-referenced-by-count":3,"title":["Mouse Tspyl5 promotes spermatogonia proliferation through enhancing Pcna-mediated DNA replication"],"prefix":"10.1071","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7046-2233","authenticated-orcid":true,"given":"Xiangyou","family":"Leng","sequence":"first","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengyu","family":"Xie","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dachang","family":"Tao","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaokun","family":"Wang","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Shi","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yi","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Tan","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7691-7630","authenticated-orcid":true,"given":"Yunqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-0312","authenticated-orcid":true,"given":"Yuan","family":"Yang","sequence":"additional","affiliation":[{"name":"ADepartment of Medical Genetics, State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"2025102820314487000_R1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/rmb2.12067","article-title":"Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR\/Cas9.","volume":"17","author":"Abbasi","year":"2018","journal-title":"Reproductive Medicine and Biology"},{"key":"2025102820314487000_R2","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.ando.2014.04.006","article-title":"Disrupting the male germ line to find infertility and contraception targets.","volume":"75","author":"Archambeault","year":"2014","journal-title":"Annales d\u2019Endocrinologie"},{"key":"2025102820314487000_R3","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1093\/humupd\/dmi006","article-title":"Fertility and ageing.","volume":"11","author":"Baird","year":"2005","journal-title":"Human Reproduction Update"},{"key":"2025102820314487000_R4","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1016\/S0015-0282(03)02161-7","article-title":"Spermatogonial proliferation patterns in men with azoospermia of different etiologies.","volume":"80","author":"Bar-Shira Maymon","year":"2003","journal-title":"Fertility and Sterility"},{"key":"2025102820314487000_R5","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1126\/science.1137741","article-title":"Male germline stem cells: from mice to men.","volume":"316","author":"Brinster","year":"2007","journal-title":"Science"},{"key":"2025102820314487000_R6","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1210\/en.2017-00653","article-title":"Sex- and age-specific impact of ERK loss within the pituitary gonadotrope in mice.","volume":"159","author":"Brown","year":"2018","journal-title":"Endocrinology"},{"key":"2025102820314487000_R7","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1038\/ng1366","article-title":"Plzf is required in adult male germ cells for stem cell self-renewal.","volume":"36","author":"Buaas","year":"2004","journal-title":"Nature Genetics"},{"key":"2025102820314487000_R8","doi-asserted-by":"publisher","first-page":"3431","DOI":"10.1093\/hmg\/ddaa233","article-title":"Unravelling the disease mechanism for TSPYL1 deficiency.","volume":"29","author":"Buyse","year":"2020","journal-title":"Human Molecular Genetics"},{"key":"2025102820314487000_R9","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1038\/ng1367","article-title":"Essential role of Plzf in maintenance of spermatogonial stem cells.","volume":"36","author":"Costoya","year":"2004","journal-title":"Nature Genetics"},{"key":"2025102820314487000_R10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102200","article-title":"Human testis-specific Y-encoded protein-like protein 5 is a histone H3\/H4-specific chaperone that facilitates histone deposition in vitro.","volume":"298","author":"Dalui","year":"2022","journal-title":"Journal of Biological Chemistry"},{"key":"2025102820314487000_R11","doi-asserted-by":"publisher","first-page":"3430","DOI":"10.1242\/dev.150284","article-title":"Spermatogonial kinetics in humans.","volume":"144","author":"Di Persio","year":"2017","journal-title":"Development"},{"key":"2025102820314487000_R12","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1012119","article-title":"Testicular aging, male fertility and beyond.","volume":"13","author":"Dong","year":"2022","journal-title":"Frontiers in Endocrinology"},{"key":"2025102820314487000_R13","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1093\/humupd\/dmk001","article-title":"Spermatogonial stem cells: questions, models and perspectives.","volume":"12","author":"Ehmcke","year":"2006","journal-title":"Human Reproduction Update"},{"key":"2025102820314487000_R14","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.molcel.2019.05.027","article-title":"TSPYL5 depletion induces specific death of ALT cells through USP7-dependent proteasomal degradation of POT1.","volume":"75","author":"Episkopou","year":"2019","journal-title":"Molecular Cell"},{"key":"2025102820314487000_R15","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1038\/ncb2142","article-title":"TSPYL5 suppresses p53 levels and function by physical interaction with USP7.","volume":"13","author":"Epping","year":"2011","journal-title":"Nature Cell Biology"},{"key":"2025102820314487000_R16","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-019-1073-x","article-title":"LINC00908 negatively regulates microRNA-483-5p to increase TSPYL5 expression and inhibit the development of prostate cancer.","volume":"20","author":"Fan","year":"2020","journal-title":"Cancer Cell International"},{"key":"2025102820314487000_R17","doi-asserted-by":"publisher","first-page":"6770","DOI":"10.1128\/MCB.00479-07","article-title":"Repression of kit expression by Plzf in germ cells.","volume":"27","author":"Filipponi","year":"2007","journal-title":"Molecular and Cellular Biology"},{"key":"2025102820314487000_R18","doi-asserted-by":"publisher","first-page":"4016","DOI":"10.1210\/jc.2009-1029","article-title":"TSPY1 copy number variation influences spermatogenesis and shows differences among Y lineages.","volume":"94","author":"Giachini","year":"2009","journal-title":"The Journal of Clinical Endocrinology & Metabolism"},{"key":"2025102820314487000_R19","doi-asserted-by":"publisher","DOI":"10.3390\/biom10040570","article-title":"Human PCNA structure, function and interactions.","volume":"10","author":"Gonz\u00e1lez-Maga\u00f1a","year":"2020","journal-title":"Biomolecules"},{"key":"2025102820314487000_R20","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.stem.2017.09.003","article-title":"Chromatin and single-cell RNA-seq profiling reveal dynamic signaling and metabolic transitions during human spermatogonial stem cell development.","volume":"21","author":"Guo","year":"2017","journal-title":"Cell Stem Cell"},{"key":"2025102820314487000_R21","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1002\/path.1711620403","article-title":"Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some, neoplasms.","volume":"162","author":"Hall","year":"1990","journal-title":"The Journal of Pathology"},{"key":"2025102820314487000_R22","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1093\/humupd\/dmx005","article-title":"Men\u2019s knowledge, attitudes and behaviours relating to fertility.","volume":"23","author":"Hammarberg","year":"2017","journal-title":"Human Reproduction Update"},{"key":"2025102820314487000_R23","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1002\/hep.31659","article-title":"DNA methylation profiling of human hepatocarcinogenesis.","volume":"74","author":"Hernandez-Meza","year":"2021","journal-title":"Hepatology"},{"key":"2025102820314487000_R24","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/0014-4827(92)90291-F","article-title":"Immortalization of germ cells and somatic testicular cells using the SV40 large T antigen.","volume":"201","author":"Hofmann","year":"1992","journal-title":"Experimental Cell Research"},{"key":"2025102820314487000_R25","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1002\/path.1587","article-title":"Pathobiological implications of the expression of markers of testicular carcinoma in situ by fetal germ cells.","volume":"203","author":"Honecker","year":"2004","journal-title":"The Journal of Pathology"},{"key":"2025102820314487000_R26","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1002\/path.1872","article-title":"Germ cell lineage differentiation in non-seminomatous germ cell tumours.","volume":"208","author":"Honecker","year":"2006","journal-title":"The Journal of Pathology"},{"key":"2025102820314487000_R27","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1089\/dna.2017.3804","article-title":"miR-19-5p enhances tumorigenesis in human colorectal cancer cells by targeting TSPYL5.","volume":"37","author":"Huang","year":"2018","journal-title":"DNA and Cell Biology"},{"key":"2025102820314487000_R28","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3892\/or.2020.7639","article-title":"TSPYL5 activates endoplasmic reticulum stress to inhibit cell proliferation, migration and invasion in colorectal cancer.","volume":"44","author":"Huang","year":"2020","journal-title":"Oncology Reports"},{"key":"2025102820314487000_R29","doi-asserted-by":"publisher","first-page":"3659","DOI":"10.1242\/dev.152413","article-title":"Unraveling transcriptome dynamics in human spermatogenesis.","volume":"144","author":"Jan","year":"2017","journal-title":"Development"},{"key":"2025102820314487000_R30","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1111\/acel.12631","article-title":"Transcriptional coactivator with PDZ-binding motif is required to sustain testicular function on aging.","volume":"16","author":"Jeong","year":"2017","journal-title":"Aging Cell"},{"key":"2025102820314487000_R31","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1006\/excr.1997.3590","article-title":"Cytoplasmic localization of cyclin D3 in seminiferous tubules during testicular development.","volume":"234","author":"Kang","year":"1997","journal-title":"Experimental Cell Research"},{"key":"2025102820314487000_R32","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1083\/jcb.130.3.661","article-title":"Members of the NAP\/SET family of proteins interact specifically with B-type cyclins.","volume":"130","author":"Kellogg","year":"1995","journal-title":"The Journal of Cell Biology"},{"key":"2025102820314487000_R33","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20200602","article-title":"Identification of PCNA-interacting protein motifs in human DNA polymerase \u03b4.","volume":"40","author":"Khandagale","year":"2020","journal-title":"Bioscience Reports"},{"key":"2025102820314487000_R34","doi-asserted-by":"publisher","first-page":"2110","DOI":"10.1093\/humrep\/dex267","article-title":"The age of fathers in the USA is rising: an analysis of 168 867 480 births from 1972 to 2015.","volume":"32","author":"Khandwala","year":"2017","journal-title":"Human Reproduction"},{"key":"2025102820314487000_R35","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1016\/j.bbrc.2010.01.045","article-title":"TSPYL5 is involved in cell growth and the resistance to radiation in A549 cells via the regulation of p21WAF1\/Cip1 and PTEN\/AKT pathway.","volume":"392","author":"Kim","year":"2010","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2025102820314487000_R36","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02303-x","article-title":"Targeting therapy-resistant lung cancer stem cells via disruption of the AKT\/TSPYL5\/PTEN positive-feedback loop.","volume":"4","author":"Kim","year":"2021","journal-title":"Communications Biology"},{"key":"2025102820314487000_R37","doi-asserted-by":"publisher","first-page":"6487","DOI":"10.1073\/pnas.0631767100","article-title":"Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells.","volume":"100","author":"Kubota","year":"2003","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"2025102820314487000_R38","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1095\/biolreprod.104.029207","article-title":"Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells.","volume":"71","author":"Kubota","year":"2004","journal-title":"Biology of Reproduction"},{"key":"2025102820314487000_R39","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3134-7","article-title":"Testis specific Y-like 5: gene expression, methylation and implications for drug sensitivity in prostate carcinoma.","volume":"17","author":"Kumar","year":"2017","journal-title":"BMC Cancer"},{"key":"2025102820314487000_R40","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/0014-4827(86)90520-3","article-title":"Expression of proliferating cell nuclear antigen (PCNA)\/cyclin during the cell cycle.","volume":"166","author":"Kurki","year":"1986","journal-title":"Experimental Cell Research"},{"key":"2025102820314487000_R41","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S1016-8478(23)13956-2","article-title":"PCNA modifications for regulation of post-replication repair pathways.","volume":"26","author":"Lee","year":"2008","journal-title":"Molecules and Cells"},{"key":"2025102820314487000_R42","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1210\/me.2012-1397","article-title":"TSPYL5 SNPs: association with plasma estradiol concentrations and aromatase expression.","volume":"27","author":"Liu","year":"2013","journal-title":"Molecular Endocrinology"},{"key":"2025102820314487000_R43","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1007\/s10456-018-9656-z","article-title":"TSPYL5-mediated inhibition of p53 promotes human endothelial cell function.","volume":"22","author":"Na","year":"2019","journal-title":"Angiogenesis"},{"key":"2025102820314487000_R44","doi-asserted-by":"publisher","DOI":"10.1139\/o06-088","article-title":"Structure and function of nucleosome assembly proteins.","volume":"84","author":"Park","year":"2006","journal-title":"Biochemistry and Cell Biology"},{"key":"2025102820314487000_R45","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.xfss.2020.08.001","article-title":"TSPYL1 regulates steroidogenic gene expression and male factor fertility in mice.","volume":"1","author":"Peng","year":"2020","journal-title":"F&S Science"},{"key":"2025102820314487000_R46","doi-asserted-by":"publisher","first-page":"R89","DOI":"10.1530\/REP-20-0633","article-title":"Healthy ageing and spermatogenesis.","volume":"161","author":"Pohl","year":"2021","journal-title":"Reproduction"},{"key":"2025102820314487000_R47","doi-asserted-by":"publisher","first-page":"11689","DOI":"10.1073\/pnas.0401194101","article-title":"Mapping of sudden infant death with dysgenesis of the testes syndrome (SIDDT) by a SNP genome scan and identification of TSPYL loss of function.","volume":"101","author":"Puffenberger","year":"2004","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"2025102820314487000_R48","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1530\/REP-09-0513","article-title":"THY1 is a conserved marker of undifferentiated spermatogonia in the pre-pubertal bull testis.","volume":"139","author":"Reding","year":"2010","journal-title":"Reproduction"},{"key":"2025102820314487000_R49","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2018-026336","article-title":"Fertility rates and the postponement of first births: a descriptive study with Finnish population data.","volume":"9","author":"Roustaei","year":"2019","journal-title":"BMJ Open"},{"key":"2025102820314487000_R50","doi-asserted-by":"publisher","first-page":"F727","DOI":"10.1152\/ajprenal.00114.2002","article-title":"Spatial repression of PCNA by p53 during kidney development.","volume":"283","author":"Saifudeen","year":"2002","journal-title":"American Journal of Physiology - Renal Physiology"},{"key":"2025102820314487000_R51","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1093\/gerona\/gly082","article-title":"Testicular aging: an overview of ultrastructural, cellular, and molecular alterations.","volume":"74","author":"Santiago","year":"2019","journal-title":"The Journals of Gerontology: Series A"},{"key":"2025102820314487000_R52","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1093\/hmg\/5.11.1801","article-title":"Testis-specific protein, Y-encoded (TSPY) expression in testicular tissues.","volume":"5","author":"Schnieders","year":"1996","journal-title":"Human Molecular Genetics"},{"key":"2025102820314487000_R53","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1095\/biolreprod.109.082156","article-title":"Partial rescue of the KIT-deficient testicular phenotype in KitW-v\/KitW-vTg(TSPY) mice.","volume":"83","author":"Sch\u00f6ner","year":"2010","journal-title":"Biology of Reproduction"},{"key":"2025102820314487000_R54","doi-asserted-by":"publisher","first-page":"1679","DOI":"10.1093\/hmg\/ddt004","article-title":"A significant effect of the TSPY1 copy number on spermatogenesis efficiency and the phenotypic expression of the gr\/gr deletion.","volume":"22","author":"Shen","year":"2013","journal-title":"Human Molecular Genetics"},{"key":"2025102820314487000_R55","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-015-0105-1","article-title":"Integrative epigenomic and genomic filtering for methylation markers in hepatocellular carcinomas.","volume":"8","author":"Shen","year":"2015","journal-title":"BMC Medical Genomics"},{"key":"2025102820314487000_R56","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0589-7","article-title":"TSPY1 suppresses USP7-mediated p53 function and promotes spermatogonial proliferation.","volume":"9","author":"Shen","year":"2018","journal-title":"Cell Death & Disease"},{"key":"2025102820314487000_R57","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1016\/j.freeradbiomed.2013.05.021","article-title":"Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress.","volume":"65","author":"Smith","year":"2013","journal-title":"Free Radical Biology and Medicine"},{"key":"2025102820314487000_R58","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1093\/molehr\/4.3.227","article-title":"The proliferation of spermatogonia in normal and pathological human seminiferous epithelium: an immunohistochemical study using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen.","volume":"4","author":"Steger","year":"1998","journal-title":"Molecular Human Reproduction"},{"key":"2025102820314487000_R59","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1016\/S0015-0282(01)02732-7","article-title":"Spermatogonial proliferation and apoptosis in hypospermatogenesis associated with nonobstructive azoospermia.","volume":"76","author":"Takagi","year":"2001","journal-title":"Fertility and Sterility"},{"key":"2025102820314487000_R60","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.stem.2007.10.023","article-title":"Stem cell defects in ATM-deficient undifferentiated spermatogonia through DNA damage-induced cell-cycle arrest.","volume":"2","author":"Takubo","year":"2008","journal-title":"Cell Stem Cell"},{"key":"2025102820314487000_R61","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1007\/BF03343780","article-title":"Lessons from knockout and transgenic mice for infertility in men.","volume":"23","author":"Venables","year":"2000","journal-title":"Journal of Endocrinological Investigation"},{"key":"2025102820314487000_R62","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1455-y","article-title":"Germline deletion of Cdyl causes teratozoospermia and progressive infertility in male mice.","volume":"10","author":"Xia","year":"2019","journal-title":"Cell Death & Disease"},{"key":"2025102820314487000_R63","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1111\/j.1432-1033.1994.tb18733.x","article-title":"Differential effect of p53 on the promoters of mouse DNA polymerase \u03b2 gene and proliferating-cell-nuclear-antigen gene.","volume":"221","author":"Yamaguchi","year":"1994","journal-title":"European Journal of Biochemistry"},{"key":"2025102820314487000_R64","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/S0015-0282(00)00634-8","article-title":"Evaluation of meiotic impairment of azoospermic men by fluorescence in situ hybridization.","volume":"74","author":"Yogev","year":"2000","journal-title":"Fertility and Sterility"},{"key":"2025102820314487000_R65","doi-asserted-by":"publisher","first-page":"623","DOI":"10.4103\/1008-682X.153851","article-title":"Advantages of using the CRISPR\/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models.","volume":"17","author":"Young","year":"2015","journal-title":"Asian Journal of Andrology"},{"key":"2025102820314487000_R66","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1093\/hmg\/1.9.717","article-title":"Molecular isolation and characterization of an expressed gene from the human Y chromosome.","volume":"1","author":"Zhang","year":"1992","journal-title":"Human Molecular Genetics"},{"key":"2025102820314487000_R67","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.124768","article-title":"Reactive oxygen species-evoked genotoxic stress mediates arsenic-induced suppression of male germ cell proliferation and decline in sperm quality.","volume":"406","author":"Zhang","year":"2021","journal-title":"Journal of Hazardous Materials"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/doi\/10.1071\/RD23042\/320409\/rd23042.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/doi\/10.1071\/RD23042\/320409\/rd23042.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:31:48Z","timestamp":1761697908000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/36\/3\/RD23042\/51074\/Mouse-Tspyl5-promotes-spermatogonia-proliferation"}},"subtitle":[],"editor":[{"given":"Jessica","family":"Dunleavy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,1,8]]},"references-count":67,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,1,25]]}},"URL":"https:\/\/doi.org\/10.1071\/rd23042","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,8]]},"article-number":"RD23042"}}