{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:17:42Z","timestamp":1761722262341,"version":"build-2065373602"},"reference-count":36,"publisher":"CSIRO Publishing","issue":"9","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":379,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Health Research Council of New Zealand, Gravida: National Centre for Growth and Development"},{"name":"The University of the Republic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,28]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>A maternal high-fat diet is thought to pose a risk to spermatogenesis in the progeny.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>We tested whether a maternal high-fat diet would affect Sertoli cell expression of transcription factors (insulin-like growth factor I (IGF-I); glial-cell line-derived neurotrophic factor (GDNF); Ets variant 5 (ETV5)) and cell proliferation and apoptotic proteins, in the testis of adult offspring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pregnant rats were fed ad libitum with a standard diet (Control) or a high-fat diet (HFat) throughout pregnancy and lactation. After weaning, male pups were fed the standard diet until postnatal day 160. Males were monitored daily from postnatal day 34 to determine onset of puberty. On postnatal day 160, their testes were processed for morphometry and immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>The HFat diet increased seminiferous-tubule diameter (P\u00a0&amp;lt;\u00a00.03), the numbers of Sertoli cells (P\u00a0&amp;lt;\u00a00.0001) and Ki-67-positive spermatogonia (P\u00a0&amp;lt;\u00a00.0006), and the areas immunostained for ETV5 (P\u00a0&amp;lt;\u00a00.0001), caspase-3 (P\u00a0&amp;lt;\u00a00.001) and Bcl-2 (P\u00a0&amp;lt;\u00a00.0001). By contrast, the HFat diet reduced the areas immunostained for IGF-I (P\u00a0&amp;lt;\u00a00.01) and GDNF (P\u00a0&amp;lt;\u00a00.0001).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A maternal high-fat diet alters the balance between spermatogonia proliferation and spermatid apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>A maternal high-fat diet seems to \u2018program\u2019 adult male fertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd23082","type":"journal-article","created":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:29:38Z","timestamp":1715556578000},"source":"Crossref","is-referenced-by-count":0,"title":["Maternal high-fat diet during pregnancy and lactation affects factors that regulate cell proliferation and apoptosis in the testis of adult progeny"],"prefix":"10.1071","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5187-5825","authenticated-orcid":true,"given":"Helen","family":"Viotti","sequence":"first","affiliation":[{"name":"AFacultad de Veterinaria, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8367-7142","authenticated-orcid":true,"given":"Daniel","family":"Cavestany","sequence":"additional","affiliation":[{"name":"AFacultad de Veterinaria, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1905-7934","authenticated-orcid":true,"given":"Graeme B.","family":"Martin","sequence":"additional","affiliation":[{"name":"BUWA School of Agriculture and Environment, and UWA Institute of Agriculture, University of Western Australia, Perth, WA, 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Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4955-6873","authenticated-orcid":true,"given":"Graciela","family":"Pedrana","sequence":"additional","affiliation":[{"name":"AFacultad de Veterinaria, Universidad de la Rep\u00fablica, Montevideo, Uruguay."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"2025102820333701700_R1","doi-asserted-by":"publisher","first-page":"783","DOI":"10.2174\/1381612822666151209151550","article-title":"Male fertility and obesity: are ghrelin, leptin and glucagon-like peptide-1 pharmacologically relevant?","volume":"22","author":"Alves","year":"2016","journal-title":"Current Pharmaceutical Design"},{"key":"2025102820333701700_R2","doi-asserted-by":"publisher","first-page":"1950","DOI":"10.1071\/RD16277","article-title":"Maternal obesity in the rat impairs male offspring aging of the testicular antioxidant defence 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