{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:15:11Z","timestamp":1761722111517,"version":"build-2065373602"},"reference-count":52,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":494,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,25]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Resumption of testicular function after gonadotrophin-releasing hormone (GnRH) immunisation varies among individual animals and some stallions regain fertility only after a prolonged time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study evaluated endocrine effects of GnRH immunisation and early subsequent re-stimulation with a GnRH agonist. We hypothesised that GnRH agonist treatment advances resumption of normal endocrine function in GnRH-vaccinated stallions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Shetland stallions were assigned to an experimental and a control group (n\u00a0=\u00a06 each). Experimental stallions were GnRH-immunised twice, 4\u00a0weeks apart. Each experimental stallion was hemicastrated together with an age-matched control animal when testosterone concentration decreased below 0.3\u00a0ng\/mL. Three weeks later, daily treatment with the GnRH agonist buserelin was initiated (4\u00a0\u03bcg\/day for 4\u00a0weeks followed by 8\u00a0\u03bcg\/day). The remaining testicle was removed when testosterone concentration exceeded 0.5\u00a0ng\/mL in vaccinated stallions. Blood was collected for LH, FSH, oestradiol and anti-m\u00fcllerian hormone (AMH) analyses, and testicular and epididymal tissue were conserved for real-time qPCR and histology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>GnRH vaccination reduced blood concentrations of LH and FSH, with a structural deterioration of testicular tissue and disruption of spermatogenesis. Daily buserelin treatment for approximately 60\u00a0days partially restored gonadotropin secretion and induced a recovery of the functional organisation of the testicular tissue with effective spermatogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Endocrine testicular function can be restored in GnRH-vaccinated stallions by daily low-dose buserelin treatment. The buserelin treatment protocol may potentially be improved regarding the dose, interval and duration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>Daily buserelin treatment can be recommended for treatment of GnRH-vaccinated stallions with prolonged inhibition of testicular function.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd23185","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T18:54:15Z","timestamp":1705604055000},"source":"Crossref","is-referenced-by-count":0,"title":["Endocrine changes induced by GnRH immunisation and subsequent early re-stimulation of testicular function with a GnRH agonist in stallions"],"prefix":"10.1071","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3604-4836","authenticated-orcid":true,"given":"Camille","family":"Gautier","sequence":"first","affiliation":[{"name":"AArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterin\u00e4rplatz 1, Vienna 1210, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rg","family":"Aurich","sequence":"additional","affiliation":[{"name":"BObstetrics, Gynecology and Andrology, Department for Small Animals and Horses, Vetmeduni Vienna, Veterin\u00e4rplatz 1, Vienna 1210, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Melchert","sequence":"additional","affiliation":[{"name":"BObstetrics, Gynecology and Andrology, Department for Small Animals and Horses, Vetmeduni Vienna, Veterin\u00e4rplatz 1, Vienna 1210, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa-H\u00e9l\u00e8ne","family":"Wagner","sequence":"additional","affiliation":[{"name":"AArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterin\u00e4rplatz 1, Vienna 1210, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martim","family":"Kaps","sequence":"additional","affiliation":[{"name":"AArtificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Veterin\u00e4rplatz 1, Vienna 1210, Austria."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina T. 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