{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T07:19:05Z","timestamp":1761722345421,"version":"build-2065373602"},"reference-count":33,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":454,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,8]]},"abstract":"<jats:p>The term \u2018mitochondrial vesicle\u2019 was first used in 2003 in a description of anuran sperm and persists to this day throughout the literature on assisted reproductive technologies (ART) for amphibians. In the present paper, we argue that the term is inappropriate because the widely accepted definition of a \u2018vesicle\u2019 refers to an integral structure with an enclosing lipid bilayer\/membrane. Moreover, there are no electron micrographs that show a vesicular structure encapsulating mitochondria on amphibian sperm heads in the literature. In fact, in 1993, the mitochondria in the anuran sperm head had been described as positioned in \u2018mitochondrial collars\u2019 or \u2018mitochondrial sheaths\u2019 surrounded by the plasma membrane of the sperm head. On the other hand, mitochondrial-derived vesicles are defined as vesicles shed from mitochondria surfaces, potentially creating confusion. Therefore, our view is that the term \u2018mitochondrial vesicle\u2019 should be avoided in describing the positioning of mitochondria on sperm.<\/jats:p>","DOI":"10.1071\/rd24014","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T17:33:09Z","timestamp":1709055189000},"source":"Crossref","is-referenced-by-count":0,"title":["The case for considering the term \u2018mitochondrial vesicle\u2019 as a misnomer in publications about assisted reproductive technologies (ART) for amphibians"],"prefix":"10.1071","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3172-9991","authenticated-orcid":true,"given":"Robert K.","family":"Browne","sequence":"first","affiliation":[{"name":"ASustainability America, La Isla Road, Sarteneja, Corozal District, Belize."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4873-7418","authenticated-orcid":true,"given":"Govindappa","family":"Venu","sequence":"additional","affiliation":[{"name":"BDepartment of Zoology, Centre for Applied Genetics, Jnana Bharathi Campus, Bangalore University, Bengaluru, Karnataka 560056, India."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2298-1597","authenticated-orcid":true,"given":"Svetlana A.","family":"Kaurova","sequence":"additional","affiliation":[{"name":"CInstitute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"2025102820343865300_R1","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1007\/BF02704111","article-title":"The sperm of Hylodinae species (Anura, Leptodactylidae): ultrastructural characteristics and their relevance to interspecific taxonomic relationships.","volume":"31","author":"Aguiar","year":"2006","journal-title":"Journal of Biosciences"},{"issue":"4\u20135","key":"2025102820343865300_R2","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1071\/rd21037","article-title":"Fertilization potential of cold-stored Fowler\u2019s toad (Anaxyrus fowleri) spermatozoa: temporal changes in sperm motility based on temperature and osmolality.","volume":"34","author":"Arregui","year":"2022","journal-title":"Reproduction, Fertility and Development"},{"issue":"3\u20134","key":"2025102820343865300_R3","first-page":"257","article-title":"An ultrastructural study of sperm of the genus Bufo (Amphibia, Anura, Bufonidae).","volume":"36","author":"Braz","year":"2004","journal-title":"Journal of Submicroscopic Cytology and Pathology"},{"issue":"1","key":"2025102820343865300_R4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.theriogenology.2014.09.018","article-title":"Sperm motility of externally fertilizing fish and amphibians.","volume":"83","author":"Browne","year":"2015","journal-title":"Theriogenology"},{"issue":"1","key":"2025102820343865300_R5","doi-asserted-by":"publisher","first-page":"coac032","DOI":"10.1093\/conphys\/coac032","article-title":"Development of an amphibian sperm biobanking protocol for genetic management and population sustainability.","volume":"10","author":"Burger","year":"2022","journal-title":"Conservation Physiology"},{"issue":"2","key":"2025102820343865300_R6","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2022.109850","article-title":"Applying sperm collection and cryopreservation protocols developed in a model amphibian to three threatened anuran species targeted for biobanking management.","volume":"277","author":"Burger","year":"2023","journal-title":"Biological Conservation"},{"key":"2025102820343865300_R7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2022.111191","article-title":"The impact of time and environmental factors on the mitochondrial vesicle and subsequent motility of amphibian sperm.","volume":"268","author":"Chen","year":"2022","journal-title":"Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology"},{"key":"2025102820343865300_R8","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1002\/jmor.20550","article-title":"A comparative ultrastructural analysis of spermatozoa in Pleurodema (Anura, Leptodactylidae, Leiuperinae).","volume":"277","author":"Cruz","year":"2016","journal-title":"Journal of Morphology"},{"key":"2025102820343865300_R9","unstructured":"Della Togna G (2015) Structural and functional characterization of the Panamanian golden frog (Atelopus zeteki) spermatozoa \u2013 impact of medium osmolality and cryopreservation on motility and cell viability. 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