{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:23:11Z","timestamp":1790346191029,"version":"4.1.0"},"reference-count":64,"publisher":"CSIRO Publishing","issue":"15","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":253,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21H02345"],"award-info":[{"award-number":["21H02345"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,26]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Understanding of central nervous system mechanisms related to age-related infertility remains limited. Fibril \u03b1-synuclein, distinct from its monomer form, is implicated in age-related diseases and propagates among neurons akin to prions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>We compared \u03b1-synuclein expression in gonadotropin-releasing hormone-expressing neurons (GnRH neurons) in the pre-optic area, arcuate nucleus, and median eminence of healthy heifers and aged cows to determine its role in age-related infertility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analysed mRNA and protein expression, along with fluorescent immunohistochemistry for GnRH and \u03b1-synuclein, followed by Congo red staining to detect amyloid deposits, and confocal microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Both mRNA and protein expressions of \u03b1-synuclein were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and western blots in bovine cortex, hippocampus, and anterior and posterior hypothalamus tissues. Significant differences in \u03b1-synuclein mRNA expression were observed in the cortex and hippocampus between young and old cows. Western blots showed five bands of \u03b1-synuclein, probably reflecting monomer, dimer, and oligomers, in the cortex, hippocampus, hypothalamus tissues, and there were significant differences in some bands between young and old cows. Bright-field and polarised light microscopy did not detect obvious amyloid deposition in aged hypothalami; however, higher-sensitive confocal microscopy unveiled strong positive signal of Congo red and \u03b1-synuclein in GnRH neurons in aged hypothalami. Additionally, \u03b1-synuclein expression was detected in immortalised GnRH neurons, GT1-7 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Alpha-synuclein was expressed in GnRH neurons, and some differences were observed between young and old hypothalami.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>Alpha-synuclein may play an important role in aging-related infertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd24033","type":"journal-article","created":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T18:59:28Z","timestamp":1726426768000},"source":"Crossref","is-referenced-by-count":3,"title":["Alpha-synuclein expression in GnRH neurons of young and old bovine hypothalami"],"prefix":"10.1071","volume":"36","author":[{"given":"Yvan 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