{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T20:21:30Z","timestamp":1772914890554,"version":"3.50.1"},"reference-count":49,"publisher":"CSIRO Publishing","issue":"8","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":203,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"funder":[{"name":"Research Management Wing of Gazipur Agricultural University","award":["30, FY 2021-2024"],"award-info":[{"award-number":["30, FY 2021-2024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,12]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>The increasing use of nanoparticles (NPs) in various consumer, agricultural, and pharmaceutical applications has raised considerable concern about their potential risks to human health and the environment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study investigated the progressive toxic effects of silver nanoparticles (AgNPs) in mouse testes after single and repeated exposure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Prepubertal male mice were exposed to AgNPs by gavage at 50, 200, and 500\u00a0mg\/kg body weight. Testis, epididymis, and serum were collected and subjected to histopathological analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Daily exposure to AgNPs for 7 and 15\u00a0days (n\u00a0=\u00a08) decreased sperm count, while increasing abnormal sperm count and testicular atrophy in a dose- and exposure-time-dependent manner. A single exposure to AgNPs at a dose of 200\u00a0mg\/kg body weight (n\u00a0=\u00a08) resulted in testicular histopathological changes and spermatogenic cell apoptosis in a time-dependent manner. The highest number of apoptotic cells was detected 24\u00a0h after exposure, whereas testicular testosterone (TT) concentrations decreased at 12 and 24\u00a0h. To explore whether AgNPs suppress TT concentrations by affecting the hypothalamus\u2013pituitary\u2013testicular (HPT) axis, we analyzed serum LH concentrations; however, no significant changes in LH levels were found.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study showed that AgNPs cause potential adverse effects on the testis, specifically, spermatogenic cell apoptosis, and impaired spermatogenesis in an exposure time- and dose-dependent manner. The testicular toxicity was not associated with suppression of the HPT axis, possibly involving other mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>These findings contribute to the broader discussion on NP safety and regulatory considerations, particularly regarding their reproductive toxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd24161","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T21:27:18Z","timestamp":1745875638000},"source":"Crossref","is-referenced-by-count":2,"title":["Spermatogenic cell apoptosis and impaired spermatogenesis in prepubertal mice: time- and dose-dependent toxicity of silver nanoparticles"],"prefix":"10.1071","volume":"37","author":[{"given":"Zannatul","family":"Maowa","sequence":"first","affiliation":[{"name":"ADepartment of Anatomy and Histology, Gazipur Agricultural University, Salna, Gazipur 1706, Bangladesh."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Sharifur","family":"Rahman","sequence":"additional","affiliation":[{"name":"ADepartment of Anatomy and Histology, Gazipur Agricultural University, Salna, Gazipur 1706, Bangladesh."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Nazmul","family":"Hoque","sequence":"additional","affiliation":[{"name":"BMolecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Salna, Gazipur 1706, Bangladesh."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Abdullah Al","family":"Mahmud","sequence":"additional","affiliation":[{"name":"ADepartment of Anatomy and Histology, Gazipur Agricultural University, Salna, Gazipur 1706, Bangladesh."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2657-9121","authenticated-orcid":true,"given":"Mohammad Shah","family":"Alam","sequence":"additional","affiliation":[{"name":"ADepartment of Anatomy and Histology, Gazipur Agricultural University, Salna, Gazipur 1706, Bangladesh."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"2025111817262801000_R1","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1007\/s10735-016-9701-4","article-title":"Evaluating the effect of silver nanoparticles on testes of adult albino rats (histological, immunohistochemical and biochemical study).","volume":"48","author":"Ahmed","year":"2017","journal-title":"Journal of Molecular 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