{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:34:52Z","timestamp":1789724092200,"version":"4.0.1"},"reference-count":31,"publisher":"CSIRO Publishing","issue":"11","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":76,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Nuffield Department of Women\u2019s and Reproductive Health, University of Oxford"},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004789","name":"John Fell Fund, University of Oxford","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004789","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014748","name":"Clarendon Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014748","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,10]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>For survivors of childhood blood cancer, fertility preservation through ovarian tissue cryopreservation (OTC) and reimplantation is not recommended because of the risk of reintroducing malignant cells. Since a robust in vitro ovarian tissue culture system does not exist for humans, new approaches are needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To investigate new approaches to in vitro follicle growth, our aim was to determine whether mouse serum (MS) could support follicle development better in mouse ovaries in vitro compared to fetal bovine serum (FBS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Neonatal ovaries were cultured for 14\u00a0days in either MS or FBS. Follicle development and health were assessed by histological and molecular analyses. Anti-M\u00fcllerian hormone (AMH) and laminin were analysed using immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>MS supported the development of primordial follicles to preantral follicles, whereas those in FBS did not develop beyond primary. Ovaries cultured in MS had fewer atretic follicles than those in FBS. There were more AMH-positive follicles in MS-cultured than in FBS-cultured ovaries, more primary follicles that were AMH-positive and, AMH-positive primary follicles contained more AMH-positive granulosa cells than those cultured in FBS. Finally, ovaries cultured in either MS or FBS contained laminin; however, the follicle basal lamina (FBL) in MS ovaries were more defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MS better supported follicle development, health, and function than did BS, indicating that MS contains additional factors important for follicle development in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>These findings demonstrated that as-yet-unknown factors exist that are important for in vitro follicle development, and we need to define them, and explore the role of these molecules in human studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd24178","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T01:31:51Z","timestamp":1750987911000},"source":"Crossref","is-referenced-by-count":1,"title":["Ovarian culture with mouse serum improves follicle development compared with fetal bovine serum, showing the importance of as yet unidentified factors in follicle growth"],"prefix":"10.1071","volume":"37","author":[{"given":"Nilay","family":"Kuscu","sequence":"first","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3958-5693","authenticated-orcid":true,"given":"Sabriya","family":"Abdul Kader","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2218-937X","authenticated-orcid":true,"given":"Eleanor R.","family":"Stephens","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5562-1451","authenticated-orcid":true,"given":"Babatomisin V.","family":"Adeniran","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar F.","family":"Ammar","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ava S.","family":"Harrison","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1080-9837","authenticated-orcid":true,"given":"Belinda KM","family":"Lo","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1798-976X","authenticated-orcid":true,"given":"Suzannah A.","family":"Williams","sequence":"additional","affiliation":[{"name":"ANuffield Department of Women\u2019s and Reproductive Health, University of Oxford, Women\u2019s Centre, Level 3, John Radcliffe Hospital, Oxford OX3 9DU, UK."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"issue":"2","key":"2025111817134712900_R1","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1002\/ar.1090940210","article-title":"Estimation of nuclear population from microtome sections.","volume":"94","author":"Abercrombie","year":"1946","journal-title":"The Anatomical Record"},{"issue":"7","key":"2025111817134712900_R2","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1093\/humrep\/deab075","article-title":"Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid.","volume":"36","author":"Adeniran","year":"2021","journal-title":"Human Reproduction"},{"issue":"4","key":"2025111817134712900_R3","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1055\/s-2006-948555","article-title":"Extracellular matrix functions in follicle maturation.","volume":"24","author":"Berkholtz","year":"2006","journal-title":"Seminars in Reproductive Medicine"},{"issue":"1","key":"2025111817134712900_R4","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1530\/RAF-20-0058","article-title":"Analysing culture methods of frozen human ovarian tissue to improve follicle survival.","volume":"2","author":"Bjarkadottir","year":"2021","journal-title":"Reproduction and Fertility"},{"issue":"6","key":"2025111817134712900_R5","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1093\/molehr\/gax016","article-title":"Further insights into the impact of mouse follicle stage on graft outcome in an artificial ovary environment.","volume":"23","author":"Chiti","year":"2017","journal-title":"Molecular Human Reproduction"},{"issue":"6","key":"2025111817134712900_R6","doi-asserted-by":"publisher","first-page":"1514","DOI":"10.1016\/j.fertnstert.2013.03.027","article-title":"Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue.","volume":"99","author":"Dolmans","year":"2013","journal-title":"Fertility and Sterility"},{"issue":"6","key":"2025111817134712900_R7","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1210\/mend.13.6.0309","article-title":"Molecular characterization of the follicle defects in the growth differentiation factor 9-deficient ovary.","volume":"13","author":"Elvin","year":"1999","journal-title":"Molecular Endocrinology"},{"issue":"1","key":"2025111817134712900_R8","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1095\/biolreprod54.1.197","article-title":"Development in vitro of mouse oocytes from primordial follicles.","volume":"54","author":"Eppig","year":"1996","journal-title":"Biology of Reproduction"},{"key":"2025111817134712900_R9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10616-021-00510-2","article-title":"Human ovarian tissue in-vitro culture: primordial follicle activation as a new strategy for female fertility preservation.","volume":"74","author":"Ghezelayagh","year":"2022","journal-title":"Cytotechnology"},{"key":"2025111817134712900_R10","doi-asserted-by":"publisher","DOI":"10.1186\/1743-1050-3-2","article-title":"Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human).","volume":"3","author":"Griffin","year":"2006","journal-title":"Journal of Experimental & Clinical Assisted Reproduction"},{"issue":"3","key":"2025111817134712900_R11","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1093\/humrep\/dead008","article-title":"Spatio-temporal remodelling of the composition and architecture of the human ovarian cortical extracellular matrix during in vitro culture.","volume":"38","author":"Grosbois","year":"2023","journal-title":"Human Reproduction"},{"issue":"1","key":"2025111817134712900_R12","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1210\/jcem.87.1.8185","article-title":"Growth differentiation factor-9 promotes the growth, development, and survival of human ovarian follicles in organ culture.","volume":"87","author":"Hreinsson","year":"2002","journal-title":"The Journal of Clinical Endocrinology & Metabolism"},{"issue":"2","key":"2025111817134712900_R13","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1530\/reprod\/118.2.221","article-title":"Ultrastructure of the basal lamina of bovine ovarian follicles and its relationship to the membrana granulosa.","volume":"118","author":"Irving-Rodgers","year":"2000","journal-title":"Reproduction"},{"issue":"8","key":"2025111817134712900_R14","doi-asserted-by":"publisher","first-page":"1538","DOI":"10.1071\/RD15524","article-title":"Analysis of in vitro follicle development during the onset of premature ovarian insufficiency in a mouse model.","volume":"29","author":"Kaune","year":"2017","journal-title":"Reproduction, Fertility and Development"},{"issue":"2","key":"2025111817134712900_R15","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1530\/rep.1.01074","article-title":"TGF-\u03b2 superfamily members and ovarian follicle development.","volume":"132","author":"Knight","year":"2006","journal-title":"Reproduction"},{"issue":"2","key":"2025111817134712900_R16","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1530\/REP-18-0115","article-title":"In vitro and in vivo mouse follicle development in ovaries and reaggregated ovaries.","volume":"157","author":"Lo","year":"2019","journal-title":"Reproduction"},{"issue":"3","key":"2025111817134712900_R17","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1093\/molehr\/gay002","article-title":"Metaphase II oocytes from human unilaminar follicles grown in a multi-step culture system.","volume":"24","author":"McLaughlin","year":"2018","journal-title":"Molecular Human Reproduction"},{"key":"2025111817134712900_R18","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28854","article-title":"Fertility assessment and treatment in adolescent and young adult cancer survivors.","volume":"70","author":"Moravek","year":"2023","journal-title":"Pediatric Blood & Cancer"},{"key":"2025111817134712900_R19","doi-asserted-by":"publisher","DOI":"10.3791\/52458","article-title":"Culture and co-culture of mouse ovaries and ovarian follicles.","volume":"97","author":"Morgan","year":"2015","journal-title":"Journal of Visualized Experiments"},{"issue":"1","key":"2025111817134712900_R20","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1530\/acta.0.0620117","article-title":"Follicle growth in the immature mouse ovary.","volume":"62","author":"Pedersen","year":"1969","journal-title":"Acta Endocrinologica"},{"issue":"3","key":"2025111817134712900_R21","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1530\/jrf.0.0170555","article-title":"Proposal for a classification of oocytes and follicles in the mouse ovary.","volume":"17","author":"Pedersen","year":"1968","journal-title":"Reproduction"},{"issue":"1","key":"2025111817134712900_R22","first-page":"98","article-title":"The development of the mouse ovary from birth to maturity.","volume":"62","author":"Peters","year":"1969","journal-title":"Acta Endocrinologica"},{"key":"2025111817134712900_R23","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1007\/s10815-012-9912-x","article-title":"The safety of transplanting cryopreserved ovarian tissue in cancer patients: a review of the literature.","volume":"30","author":"Rosendahl","year":"2013","journal-title":"Journal of Assisted Reproduction and Genetics"},{"issue":"3","key":"2025111817134712900_R24","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1016\/S0015-0282(16)47728-9","article-title":"Effect of variable concentration of serum on mouse embryo development.","volume":"41","author":"Saito","year":"1984","journal-title":"Fertility and Sterility"},{"key":"2025111817134712900_R25","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-4-19","article-title":"Oocyte\u2013granulosa cell interactions during mouse follicular development: regulation of kit ligand expression and its role in oocyte growth.","volume":"4","author":"Thomas","year":"2006","journal-title":"Reproductive Biology and Endocrinology"},{"issue":"3","key":"2025111817134712900_R26","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.ando.2010.02.016","article-title":"Anti-M\u00fcllerian hormone (AMH): regulator and marker of ovarian function.","volume":"71","author":"Van Houten","year":"2010","journal-title":"Annales d\u2019Endocrinologie"},{"issue":"1\u20132","key":"2025111817134712900_R27","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/j.mce.2004.09.008","article-title":"Anti-M\u00fcllerian hormone and folliculogenesis.","volume":"234","author":"Visser","year":"2005","journal-title":"Molecular and Cellular Endocrinology"},{"issue":"3","key":"2025111817134712900_R28","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1080\/14647273.2019.1616118","article-title":"Variation in follicle health and development in cultured cryopreserved ovarian cortical tissue: a study of ovarian tissue from patients undergoing fertility preservation.","volume":"24","author":"Walker","year":"2021","journal-title":"Human Fertility"},{"issue":"1","key":"2025111817134712900_R29","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1093\/toxsci\/kfz022","article-title":"Vincristine chemotherapy induces atresia of growing ovarian follicles in mice.","volume":"169","author":"Winship","year":"2019","journal-title":"Toxicological Sciences"},{"key":"2025111817134712900_R30","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1093\/humrep\/deab003","article-title":"Matrix-free 3D culture supports human follicular development from the unilaminar to the antral stage in vitro yielding morphologically normal metaphase II oocytes.","volume":"36","author":"Xu","year":"2021","journal-title":"Human Reproduction"},{"issue":"4","key":"2025111817134712900_R31","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1530\/REP-10-0094","article-title":"Theca: the forgotten cell of the ovarian follicle.","volume":"140","author":"Young","year":"2010","journal-title":"Reproduction"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/doi\/10.1071\/RD24178\/1361716\/rd24178.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/doi\/10.1071\/RD24178\/1361716\/rd24178.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T22:13:55Z","timestamp":1763504035000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/37\/11\/RD24178\/200621\/Ovarian-culture-with-mouse-serum-improves-follicle"}},"subtitle":[],"editor":[{"given":"Jennifer","family":"Juengel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,6,27]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,7,10]]}},"URL":"https:\/\/doi.org\/10.1071\/rd24178","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,27]]},"article-number":"RD24178"}}