{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T22:28:41Z","timestamp":1763504921322,"version":"3.45.0"},"reference-count":45,"publisher":"CSIRO Publishing","issue":"10","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":173,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,20]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Obesity, a metabolic syndrome that results in excess fat accumulation in the body, is one of the most common diseases today, and its molecular background remains unclear. Studies have shown that a high-fat diet-induced obesity can cause changes in gene expression by affecting epigenetics in germ cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Our study aims to show the changes in DNA methyltransferase 3b (Dnmt3b) and DNA methyltransferase 3l (Dnmt3l) in the testes of obese C57BL\/6 mice fed with a high-fat diet.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The presence and quantitative differences of Dnmt3b and Dnmt3l enzymes in the testes of C57BL\/6 mice fed with a high-fat diet were demonstrated using the immunohistochemistry and Western blotting method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Our findings showed that high-fat diet-induced obesity led to a significant upregulation of Dnmt3b expression levels in the mice testes. In contrast, Dnmt3l expression was dramatically downregulated in the testes of high-fat diet-induced obese mice. Specifically, a significant increase in the expression level of Dnmt3b was observed in the all testicular cells of high-fat diet group compared with the control group, whereas on the contrary, a decrease in the expression level of Dnmt3l was observed only in spermatogonium and spermatocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>High-fat diets altered the expression levels of Dnmt3b and Dnmt3l proteins in the testes of mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>We think that our results have important value in showing the effects of obesity on spermatogenesis and may bring a new perspective to obesity-related male infertility treatments.<\/jats:p>\n                  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