{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T11:40:32Z","timestamp":1770118832438,"version":"3.49.0"},"reference-count":37,"publisher":"CSIRO Publishing","issue":"3","license":[{"start":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T00:00:00Z","timestamp":1768003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,19]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>A key endocannabinoid, 2-arachidonoyl glycerol (2-AG), regulates sperm hyperactivation in humans via alpha\/beta hydrolase domain-containing protein 2 (ABHD2)-mediated CatSper calcium channel activation. However, the role of ABHD2 in mice is unclear, whereas monoacylglycerol lipase (MAGL), ABHD-containing protein 6 (ABHD6), and ABHD-containing protein 12 (ABHD12) modulate 2-AG in the mouse central nervous system (CNS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study explored the impact of 2-AG on mouse sperm motility, its regulatory mechanisms, and the involvement of MAGL, ABHD6, and ABHD12.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>2-AG concentrations in caput and caudal epididymal sperm from Kunming mice were quantified. ABHD2\/6\/12 and MAGL were identified, localized, and assessed for roles in motility and calcium (Ca2+) influx.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Caput sperm exhibited higher 2-AG contents than did caudal sperm. Four hydrolases regulated motility via 2-AG hydrolysis to induce calcium influx. MAGL showed the strongest hydrolytic activity, ABHD2 the weakest. Subcellular localization showed MAGL in mid-piece, ABHD12 in mid-piece and principal piece, and ABHD2\/ABHD6 in acrosomal region.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>2-AG modulates mouse sperm motility via calcium signaling. MAGL, ABHD6, and ABHD12 drive 2-AG hydrolysis, while ABHD2 is linked to acrosome exocytosis, not 2-AG breakdown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>Targeting MAGL\/ABHD12 offers non-hormonal contraceptive strategies or assisted reproductive technology (ART) improvements. Monitoring 2-AG and hydrolase activity could aid in diagnosing male infertility.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd25040","type":"journal-article","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T19:26:29Z","timestamp":1768073189000},"source":"Crossref","is-referenced-by-count":0,"title":["Investigation of endocannabinoid 2-AG and its hydrolases for regulation on sperm motility and acrosome reaction in Kunming mice"],"prefix":"10.1071","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9323-1570","authenticated-orcid":true,"given":"Xin","family":"Zhang","sequence":"first","affiliation":[{"name":"AFaculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Wu","sequence":"additional","affiliation":[{"name":"BNanjing Genscript Biotech Corporation\u200c, Nanjing 210000, 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