{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:30:12Z","timestamp":1773955812803,"version":"3.50.1"},"reference-count":171,"publisher":"CSIRO Publishing","issue":"15","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":56,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,2]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Platelet-rich plasma (PRP) is a plasma component containing high concentrations of platelets, growth factors, antioxidants and proliferative properties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To mitigate the negative effects of cryopreservation on ram semen by utilising PRP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Semen was collected from six rams twice a week for 3\u00a0weeks during the breeding season. Pooling was performed by dilution with tris\u00a0+\u00a0egg yolk diluent. Pooling was divided into three equal parts and re-diluted with diluents containing control (0% PRP), 5% PRP and 10% PRP. Sperms were frozen in an automatic freezing device and stored in liquid nitrogen. After thawing, spermatological, flow-cytometric, oxidative stress, cholesterol, fatty acid, ELISA, quantitative real-time polymerase chain reaction (qRT\u2013PCR) and western blot analyses were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Compared with the control group, the 5% PRP group exhibited a significant increase in progressive motility, viability and cholesterol ratios, glutathione-peroxidase activity, CATSPER1 (Cation Channel of Sperm), CATSPER3, vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) levels and KCNJ11 (Potassium Channel, Inwardly Rectifying, Subfamily J, Member 11), HSA-MIR-181A, HSA-MIR-150 and HSA-MIR-374 transcripts. Apoptotic protein, malondialdehyde and HSA-MIR-410, OAR-MIR-10B, BTA-MIR-22-3P and RNO-MIR-494 transcripts were decreased in 5% PRP group compared with control. PRP supplementation at 10% increased dead sperm and heptadecenoic acid ratios, VEGF and PDGF levels and HSA-MIR-410, PPY-MIR-16, CFA-MIR-199, HSA-MIR-181A, HSA-MIR-150, OAR-MIR-127, HSA-LET-7A and HSA-MIR-374 transcripts as well as CATSPER3, HSD3\u03b22, PDGFB and VEGFA proteins compared with the control. PRP supplementation at 10% significantly decreased plasma membrane integrity, insulin-like growth factor 1 (IGF1) level and CATSPER3 and KCNJ11 transcripts compared with the control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The addition of 5% PRP before cryopreservation has beneficial effects on the functional and molecular properties of frozen-thawed ram spermatozoa, whereas the addition of 10% PRP has negative effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>Five per cent PRP should be added to ram semen diluents.<\/jats:p>\n                  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