{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:13:51Z","timestamp":1788401631597,"version":"build-2803163510"},"reference-count":30,"publisher":"CSIRO Publishing","issue":"13","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Trophectoderm (TE) biopsy combined with embryo vitrification is increasingly being used; however, concerns remain regarding its effects on embryo survival and physiological stability after warming.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>We aimed to evaluate whether TE biopsy performed prior to vitrification compromises early post-warming recovery and physiological responses of bovine embryos.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Expanded blastocysts were allocated into two groups, namely, Control and Biopsy. After warming, embryos were evaluated at 0, 4, 24, and 48\u00a0h for re-expansion and hatching rates. Mitochondrial activity, oxidative stress, antioxidant activity estimated by intracellular thiol content, and total cell number were assessed after 48\u00a0h.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key results<\/jats:title>\n                    <jats:p>Re-expansion differed between groups at 0\u00a0h (Control 13.40% vs Biopsy 33.90%; P\u00a0=\u00a00.005) and 4\u00a0h (Control 93.81% vs Biopsy 84.75%; P\u00a0=\u00a00.038), with no differences at 24 or 48\u00a0h (P\u00a0\u2265\u00a00.346). Hatching rates were similar at 4\u00a0h (P\u00a0=\u00a00.447), but higher in the Biopsy group at 24\u00a0h (77.56% vs 53.35%; P\u00a0=\u00a00.005) and 48\u00a0h (90.79% vs 77.39%; P\u00a0=\u00a00.027). The Biopsy group showed higher mitochondrial activity (P\u00a0&amp;lt;\u00a00.001), reactive oxygen species (P\u00a0=\u00a00.009), intracellular thiol content (P\u00a0&amp;lt;\u00a00.001), and total cell number (216.68\u00a0\u00b1\u00a09.75 vs 186.82\u00a0\u00b1\u00a010.22; P\u00a0=\u00a00.038).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>TE biopsy prior to vitrification induced oxidative stress, increased mitochondrial activity and increased thiol content, but did not compromise early post-warming recovery of bovine embryos.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Implications<\/jats:title>\n                    <jats:p>TE biopsy before vitrification is safe under short-term in vitro conditions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1071\/rd26017","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:31:16Z","timestamp":1788399076000},"source":"Crossref","is-referenced-by-count":0,"title":["Trophectoderm biopsy and vitrification: survival, oxidative stress and cell count in bovine embryos"],"prefix":"10.1071","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8346-7613","authenticated-orcid":true,"given":"Dayana Silva","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"ADepartment of Veterinary Clinical and Surgical Sciences, School of Veterinary Medicine, Federal University of Minas Gerais, 6627 Ant\u00f4nio Carlos Avenue, Belo Horizonte, Minas Gerais 31270-901, Brazil. Email: araujo.ds@hotmail.com; gabrielaclarindo2009@hotmail.com; leticiazoccolaro@yahoo.com.br"},{"name":"BEmbrapa Gado de Leite, 610 Eug\u00eanio do Nascimento, Juiz de Fora, Minas Gerais 36038-330, Brazil. Email: naiara.saraiva@embrapa.br; luiz.camargo@embrapa.br"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8652-381X","authenticated-orcid":true,"given":"Rafael Monteiro","family":"dos Santos","sequence":"additional","affiliation":[{"name":"CDepartment of Animal Science, School of Veterinary Medicine, Federal University of Minas Gerais, 6627 Ant\u00f4nio Carlos Avenue, Belo Horizonte, Minas Gerais 31270-901, Brazil. Email: rmtds.97@gmail.com"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8654-5201","authenticated-orcid":true,"given":"Gabriela Luiza Soares","family":"Clarindo","sequence":"additional","affiliation":[{"name":"ADepartment of Veterinary Clinical and Surgical Sciences, School of Veterinary Medicine, Federal University of Minas Gerais, 6627 Ant\u00f4nio Carlos Avenue, Belo Horizonte, Minas Gerais 31270-901, Brazil. Email: araujo.ds@hotmail.com; gabrielaclarindo2009@hotmail.com; leticiazoccolaro@yahoo.com.br"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7478-3664","authenticated-orcid":true,"given":"Naiara Zoccal","family":"Saraiva","sequence":"additional","affiliation":[{"name":"BEmbrapa Gado de Leite, 610 Eug\u00eanio do Nascimento, Juiz de Fora, Minas Gerais 36038-330, Brazil. Email: naiara.saraiva@embrapa.br; luiz.camargo@embrapa.br"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9974-711X","authenticated-orcid":true,"given":"Luiz Sergio de Almeida","family":"Camargo","sequence":"additional","affiliation":[{"name":"BEmbrapa Gado de Leite, 610 Eug\u00eanio do Nascimento, Juiz de Fora, Minas Gerais 36038-330, Brazil. Email: naiara.saraiva@embrapa.br; luiz.camargo@embrapa.br"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6660-9191","authenticated-orcid":true,"given":"Leticia Zoccolaro","family":"Oliveira","sequence":"additional","affiliation":[{"name":"ADepartment of Veterinary Clinical and Surgical Sciences, School of Veterinary Medicine, Federal University of Minas Gerais, 6627 Ant\u00f4nio Carlos Avenue, Belo Horizonte, Minas Gerais 31270-901, Brazil. 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