{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:01:41Z","timestamp":1767859301468,"version":"3.49.0"},"reference-count":0,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":9476,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,6,17]]},"abstract":"<jats:p>The peri-implantation development involves zona escape (hatching) of blastocysts and their attachment and proliferation. These events are difficult to study in vivo , so in this study hamster 8-cell embryos were cultured through the hatched and attached blastocyst stages using different formulations of hamster embryo culture medium (HECM)-2. Supplementation of succinate, amino acids, vitamins (inositol, pantothenate, choline chloride) and bovine serum albumin (BSA) to HECM-2 supported 100% development of \u2018zona-escaped\u2019 blastocysts. In this medium (designated as hatching, i.e. HECM-2h) all blastocysts invariably deflated and escaped from focally lysed zonae, which underwent complete dissolution. In their presence, pre-morula stage embryos also escaped from zonae. Omission of BSA from HECM-2h failed to support zona escape whereas that of vitamins reduced zona escape (34.0% 7.0). Blastocysts with the potential to undergo zona escape in HECM-2h were of high quality as they had a higher mean cell number (MCN) than the MCN of those developing in BSA-free HECM-2h (35.2 1.6 v. 24.3 1.1). Cell allocation (i.e. trophectoderm to inner cell mass ratio) in blastocysts remained unaltered in both media (2.6 0.2 v. 2.7 0.2). Supplementation of 10% bovine fetal serum (BFS) to HECM-2h was detrimental to the development of blastocysts (22.3% 7.4) and none of them underwent zona escape. Interestingly, BFS was required either as a supplement to the medium or as a coating on dishes for azonal blastocysts to attach (=70%) and exhibit trophoblast (TB) outgrowth (30.3 \u00d7 103 2.9 \u00d7 103 \u00b5m2 at 48 h). These results show that HECM-2h supports maximal development of zona-escaped blastocysts with the potential to attach and exhibit TB outgrowth, and there is a developmental stage-specific requirement for serum during peri-attachment in hamster development.<\/jats:p>","DOI":"10.1071\/rd98100","type":"journal-article","created":{"date-parts":[[2003,11,16]],"date-time":"2003-11-16T22:35:35Z","timestamp":1069022135000},"page":"413-420","source":"Crossref","is-referenced-by-count":20,"title":["Successful development\n                    <i>in vitro<\/i>\n                    of hamster 8-cell embryos to \u2018zona-escaped\u2019 and attached blastocysts: assessment of quality and trophoblast outgrowth"],"prefix":"10.1071","volume":"10","author":[{"given":"Archana","family":"Mishra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. B.","family":"Seshagiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/10\/5\/413\/304716\/rd98100.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/10\/5\/413\/304716\/rd98100.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:03:05Z","timestamp":1761692585000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/10\/5\/413\/49072\/Successful-development-in-vitro-of-hamster-8-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,6,17]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,6,17]]}},"URL":"https:\/\/doi.org\/10.1071\/rd98100","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,6,17]]}}}