{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:34:43Z","timestamp":1792002883713,"version":"4.4.0"},"reference-count":33,"publisher":"CSIRO Publishing","issue":"1","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":6391,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,2,22]]},"abstract":"<jats:p>Nucleic acid detection tests (NADT) have considerable benefits for the detection of Neisseria gonorrhoeae (GC), including high sensitivity across a range of specimen types and use under widely differing settings and conditions. However, sexual health practitioners and others who use data generated by NADT for GC should be aware of some important limitations of these tests. False-positive results caused by cross reaction with commensal Neisseria species have been observed in many assays, and have lead to unacceptably low positive-predictive values in some patient populations. Further, false-negative results can be caused by GC sequence variation, with some gonococci lacking certain NADT target sequences. This review examines the issues associated with gonococcal NADT and considers best practice for use of these assays based on current knowledge. We emphasise the need for supplementary testing and extensive assay validation, and suggest appropriate strategies for these requirements irrespective of the setting in which they are used. Further, we highlight the need to maintain culture-based testing for certain specimen sites as well as for antimicrobial resistance surveillance.<\/jats:p>","DOI":"10.1071\/sh07050","type":"journal-article","created":{"date-parts":[[2008,2,21]],"date-time":"2008-02-21T19:28:40Z","timestamp":1203622120000},"page":"17-23","source":"Crossref","is-referenced-by-count":44,"title":["Exploring \u2018best practice\u2019 for nucleic acid detection of Neisseria gonorrhoeae"],"prefix":"10.1071","volume":"5","author":[{"given":"David M.","family":"Whiley","sequence":"first","affiliation":[{"name":"AQueensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children\u2019s Hospital and Health Service District, Brisbane, Qld 4029, Australia."},{"name":"BClinical Medical Virology Centre, University of Queensland, Qld 4029, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne M.","family":"Garland","sequence":"additional","affiliation":[{"name":"CDepartment of Microbiology and Infectious Diseases, The Royal Womens\u2019 Hospital, Melbourne, Vic. 3053, Australia."},{"name":"DDepartment of Obstetrics and Gynaecology, University of Melbourne, Vic. 3053, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Harnett","sequence":"additional","affiliation":[{"name":"EDivision of Microbiology and Infectious Diseases, PathWest Laboratory Medicine Nedlands, WA 6009, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Lum","sequence":"additional","affiliation":[{"name":"FMicrobiology Laboratory, Pathology Department, Royal Darwin Hospital, Casuarina, NT 0810, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Smith","sequence":"additional","affiliation":[{"name":"EDivision of Microbiology and Infectious Diseases, PathWest Laboratory Medicine Nedlands, WA 6009, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepehr N.","family":"Tabrizi","sequence":"additional","affiliation":[{"name":"CDepartment of Microbiology and Infectious Diseases, The Royal Womens\u2019 Hospital, Melbourne, Vic. 3053, Australia."},{"name":"DDepartment of Obstetrics and Gynaecology, University of Melbourne, Vic. 3053, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo P.","family":"Sloots","sequence":"additional","affiliation":[{"name":"AQueensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children\u2019s Hospital and Health Service District, Brisbane, Qld 4029, Australia."},{"name":"BClinical Medical Virology Centre, University of Queensland, Qld 4029, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Tapsall","sequence":"additional","affiliation":[{"name":"GWHO Collaborating Centre for STD and HIV, Microbiology Department, The Prince of Wales Hospital, Randwick, NSW 2031, Australia."},{"name":"HCorresponding author. Email: j.tapsall@unsw.edu.au"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"67","published-online":{"date-parts":[[2008,2,22]]},"reference":[{"key":"2025102616210180700_R1"},{"key":"2025102616210180700_R2"},{"key":"2025102616210180700_R3"},{"key":"2025102616210180700_R4"},{"key":"2025102616210180700_R5"},{"key":"2025102616210180700_R6"},{"key":"2025102616210180700_R7"},{"key":"2025102616210180700_R8"},{"key":"2025102616210180700_R9"},{"key":"2025102616210180700_R10"},{"key":"2025102616210180700_R11"},{"key":"2025102616210180700_R12"},{"key":"2025102616210180700_R13"},{"key":"2025102616210180700_R14"},{"key":"2025102616210180700_R15"},{"key":"2025102616210180700_R16"},{"key":"2025102616210180700_R17"},{"key":"2025102616210180700_R18"},{"key":"2025102616210180700_R19"},{"key":"2025102616210180700_R20"},{"key":"2025102616210180700_R21"},{"key":"2025102616210180700_R22"},{"key":"2025102616210180700_R23"},{"key":"2025102616210180700_R24"},{"key":"2025102616210180700_R25"},{"key":"2025102616210180700_R26"},{"key":"2025102616210180700_R27"},{"key":"2025102616210180700_R28"},{"key":"2025102616210180700_R29"},{"key":"2025102616210180700_R30"},{"key":"2025102616210180700_R31"},{"key":"2025102616210180700_R32"},{"key":"2025102616210180700_R33"}],"container-title":["Sexual Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/sh\/article-pdf\/5\/1\/17\/1021395\/sh07050.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/sh\/article-pdf\/5\/1\/17\/1021395\/sh07050.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:21:07Z","timestamp":1761510067000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/sh\/article\/5\/1\/17\/128063\/Exploring-best-practice-for-nucleic-acid-detection"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,2,22]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,2,22]]}},"URL":"https:\/\/doi.org\/10.1071\/sh07050","relation":{},"ISSN":["1448-5028","1449-8987"],"issn-type":[{"value":"1448-5028","type":"print"},{"value":"1449-8987","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,2,22]]}}}