{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T08:40:45Z","timestamp":1772613645954,"version":"3.50.1"},"reference-count":9,"publisher":"CSIRO Publishing","issue":"4","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":3014,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,7]]},"abstract":"<jats:p>porA pseudogene-negative Neisseria gonorrhoeae isolates produce false-negative results when examined by polymerase chain reaction (PCR) with porA pseudogene targets. In the present study, 533 representative gonococcal isolates received in 2011 via the Gonococcal Resistance to Antimicrobials Surveillance Program were examined to determine the prevalence of porA-negative isolates. Less than 0.4% (2\/533) of isolates were found to be reproducibly negative with the porA real-time PCR but were confirmed as N. gonorrhoeae with molecular, biochemical and immunological confirmatory tests. Sequencing revealed both isolates contained the Neisseria meningitidis porA gene. Low prevalence indicates that although these isolates do not present a major public health problem, microbiologists should remain vigilant.<\/jats:p>","DOI":"10.1071\/sh16162","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T20:17:29Z","timestamp":1495052249000},"page":"392-393","source":"Crossref","is-referenced-by-count":9,"title":["Prevalence of porA pseudogene deletion among Neisseria gonorrhoeae isolates referred to the UK\u2019s Gonococcal Resistance to Antimicrobials Surveillance Program"],"prefix":"10.1071","volume":"14","author":[{"given":"Martina","family":"Toby","sequence":"first","affiliation":[{"name":"A56 Dean Street, Soho, London, W1D 6AQ, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Saunders","sequence":"additional","affiliation":[{"name":"BSexually Transmitted Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Cole","sequence":"additional","affiliation":[{"name":"BSexually Transmitted Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vlad","family":"Grigorjev","sequence":"additional","affiliation":[{"name":"BSexually Transmitted Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Alexander","sequence":"additional","affiliation":[{"name":"BSexually Transmitted Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK."},{"name":"CCorresponding author. Email: Sarah.Alexander@phe.gov.uk"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Ison","sequence":"additional","affiliation":[{"name":"BSexually Transmitted Bacteria Reference Unit, Public Health England, 61 Colindale Avenue, London, NW9 5EQ, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"67","published-online":{"date-parts":[[2017,5,18]]},"reference":[{"key":"2025102615584819100_R1","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1136\/sextrans-2014-051749","article-title":"Survey of the laboratory diagnosis of gonorrhoea and chlamydial infection in the UK.","volume":"91","author":"Toby","year":"2015","journal-title":"Sex Transm Infect"},{"key":"2025102615584819100_R2","doi-asserted-by":"crossref","first-page":"20102","DOI":"10.2807\/ese.17.09.20102-en","article-title":"Clinical Neisseria gonorrhoeae isolate with a N. meningitidis porA gene and no prolyliminopeptidase activity, Sweden, 2011 \u2013 danger of false-negative genetic and culture diagnostic results.","volume":"17","author":"Golparian","year":"2012","journal-title":"Euro Surveill"},{"key":"2025102615584819100_R3","doi-asserted-by":"crossref","first-page":"20101","DOI":"10.2807\/ese.17.09.20101-en","article-title":"Identification of Neisseria gonorrhoeae isolates with a recombinant porA gene in Scotland, United Kingdom, 2010 to 2011.","volume":"17","author":"Eastick","year":"2012","journal-title":"Euro Surveill"},{"key":"2025102615584819100_R4","doi-asserted-by":"crossref","first-page":"19874","DOI":"10.2807\/ese.16.21.19874-en","article-title":"False-negative results using Neisseria gonorrhoeae porA pseudogene PCR \u2013 a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011.","volume":"16","author":"Whiley","year":"2011","journal-title":"Euro Surveill"},{"key":"2025102615584819100_R5","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1136\/sextrans-2012-050829","article-title":"Evolution of Neisseria gonorrhoeae is a continuing challenge for molecular detection of gonorrhoeae: false negative gonococcal porA mutants are spreading internationally.","volume":"89","author":"Ison","year":"2013","journal-title":"Sex Transm Infect"},{"key":"2025102615584819100_R6","unstructured":"Health Protection Agency. GRASP 2011 Report: the gonococcal resistance to antimicrobials surveillance programme. 2012. Available online at: http:\/\/webarchive.nationalarchives.gov.uk\/20140714084352\/http:\/\/www.hpa.org.uk\/webc\/HPAwebFile\/HPAweb_C\/1317136030908 [verified 23 April 2017]."},{"key":"2025102615584819100_R7","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1080\/00313020500254552","article-title":"Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies.","volume":"37","author":"Whiley","year":"2005","journal-title":"Pathology"},{"key":"2025102615584819100_R8","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1097\/01.olq.0000154495.24519.bf","article-title":"Evaluation of opa-based real-time PCR for detection of Neisseria gonorrhoeae.","volume":"32","author":"Tabrizi","year":"2005","journal-title":"Sex Transm Dis"},{"key":"2025102615584819100_R9","unstructured":"Public Health England. Guidance for the detection of gonorrhoea in England 2014. 2014. Available online at: https:\/\/www.gov.uk\/government\/uploads\/system\/uploads\/attachment_data\/file\/405293\/170215_Gonorrhoea_testing_guidance_REVISED__2_.pdf [verified 23 April 2017]."}],"container-title":["Sexual Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/sh\/article-pdf\/14\/4\/392\/845304\/sh16162.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/sh\/article-pdf\/14\/4\/392\/845304\/sh16162.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T19:58:53Z","timestamp":1761508733000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/sh\/article\/14\/4\/392\/106969\/Prevalence-of-porA-pseudogene-deletion-among"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,18]]},"references-count":9,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,8,7]]}},"URL":"https:\/\/doi.org\/10.1071\/sh16162","relation":{},"ISSN":["1448-5028","1449-8987"],"issn-type":[{"value":"1448-5028","type":"print"},{"value":"1449-8987","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,18]]}}}