{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:40:09Z","timestamp":1773661209788,"version":"3.50.1"},"reference-count":53,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4715,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,7,12]]},"abstract":"<jats:p>We investigated the use of spectral reflectance techniques to monitor the physiological responses of Ceratonia siliqua L. seedlings exposed to different levels of water availability under normal (25 : 18\u00b0C, day : night) and elevated (32 : 21\u00b0C, day : night) temperatures. Three spectral reflectance indices (photochemical reflectance index, PRI; water index, WI; red edge position, REP) were measured along with water status, chlorophyll fluorescence and chlorophyll concentration variables in the leaves of well watered, moderately stressed, severely stressed and rehydrated plants under each temperature regime. The PSII effective photochemical efficiency (f2) and the intrinsic efficiency of open PSII centres (F'v\/F'm) correlated significantly with PRI, and these three variables loaded heavily onto the same principal component of a three-factor principal component analysis solution. Water concentration (WC) and the succulence index (SI) were more strongly correlated with WI than either water potential (?PD) or relative water content (RWC). Accordingly, WI, WC and SI were combined in the second principal component, and ?PD and RWC in the third. Our results provide clear evidence for interaction between water availability and temperature in the WI and the PRI response segments of the reflectance curves. Elevated temperature inhibited the recovery of WI spectral segments more than that of the PRI segments in SS plants. REP showed a strongly positive linear relationship with leaf total chlorophyll concentration across all water and temperature treatment combinations. PRI, WI and REP are therefore reliable markers that can be used to monitor f2, WC and total chlorophyll concentration, respectively, in C. siliqua seedlings under drought and temperature stress.<\/jats:p>","DOI":"10.1071\/fp11284","type":"journal-article","created":{"date-parts":[[2014,6,27]],"date-time":"2014-06-27T01:32:33Z","timestamp":1403832753000},"page":"588-597","source":"Crossref","is-referenced-by-count":17,"title":["Reflectance indices as nondestructive indicators of the physiological status of Ceratonia siliqua seedlings under varying moisture and temperature regimes"],"prefix":"10.1071","volume":"39","author":[{"given":"J\u00falio","family":"Os\u00f3rio","sequence":"first","affiliation":[{"name":"AInstitute of Mediterranean Agricultural and Environmental Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 8, Campus de Gambelas, 8005-139 Faro, Portugal."},{"name":"CCorresponding author. Email: josorio@ualg.pt"}]},{"given":"Maria Leonor","family":"Os\u00f3rio","sequence":"additional","affiliation":[{"name":"BInstitute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Faculty of Sciences and Technology, University of Algarve, Ed. 8, Campus de Gambelas, 8005-139 Faro, Portugal."}]},{"given":"Anabela","family":"Romano","sequence":"additional","affiliation":[{"name":"BInstitute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, Faculty of Sciences and Technology, University of Algarve, Ed. 8, Campus de Gambelas, 8005-139 Faro, Portugal."}]}],"member":"67","published-online":{"date-parts":[[2012,6,29]]},"reference":[{"key":"2025102218195477900_R1","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1071\/BI9620413","article-title":"A re-examination of the relative turgidity technique for estimating water deficits in leaves.","volume":"15","author":"Barrs","year":"1962","journal-title":"Australian Journal of Biological Sciences"},{"key":"2025102218195477900_R2","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1071\/FP08043","article-title":"Biochemical constrains limit the potential of the photochemical reflectance index as a predictor of effective quantum efficiency of photosynthesis during the winter spring transition in Jack pine seedlings.","volume":"36","author":"Busch","year":"2009","journal-title":"Functional Plant Biology"},{"key":"2025102218195477900_R3","doi-asserted-by":"publisher","first-page":"239","DOI":"10.2307\/2445346","article-title":"Responses of leaf spectral reflectance to plant stress.","volume":"80","author":"Carter","year":"1993","journal-title":"American Journal of Botany"},{"key":"2025102218195477900_R4"},{"key":"2025102218195477900_R5","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1093\/treephys\/7.1-2-3-4.33","article-title":"Exploring the relationship between reflectance red edge and chlorophyll content in slash pine.","volume":"7","author":"Curran","year":"1990","journal-title":"Tree Physiology"},{"key":"2025102218195477900_R6","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1093\/treephys\/15.3.203","article-title":"Exploring the relationship between reflectance red edge and chlorophyll concentration in slash pine leaves.","volume":"15","author":"Curran","year":"1995","journal-title":"Tree Physiology"},{"key":"2025102218195477900_R7","doi-asserted-by":"publisher","first-page":"2133","DOI":"10.1080\/014311698214910","article-title":"A new technique for interpolating the reflectance red edge position.","volume":"19","author":"Dawson","year":"1998","journal-title":"International Journal of Remote Sensing"},{"key":"2025102218195477900_R8","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1146\/annurev.pp.43.060192.003123","article-title":"Photoprotection and other responses of plants to light stress.","volume":"43","author":"Demmig-Adams","year":"1992","journal-title":"Annual Review of Plant Physiology and Plant Molecular Biology"},{"key":"2025102218195477900_R9","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/S1360-1385(96)80019-7","article-title":"The role of xanthophyll cycle carotenoids in the protection of photosynthesis.","volume":"1","author":"Demmig-Adams","year":"1996","journal-title":"Trends in Plant Science"},{"key":"2025102218195477900_R10","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1080\/01431169408954177","article-title":"The red edge position and shape as indicators of plant chlorophyll content, biomass potential for land applications.","volume":"15","author":"Filella","year":"1994","journal-title":"International Journal of Remote Sensing"},{"key":"2025102218195477900_R11","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1046\/j.1469-8137.1999.00424.x","article-title":"Assessing leaf pigment content and activity with a reflectometer.","volume":"143","author":"Gamon","year":"1999","journal-title":"New Phytologist"},{"key":"2025102218195477900_R12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00317336","article-title":"Remote sensing of the xanthophyll cycle and chlorophyll fluorescence in sunflower leaves and canopies.","volume":"85","author":"Gamon","year":"1990","journal-title":"Oecologia"},{"key":"2025102218195477900_R13","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/0034-4257(92)90059-S","article-title":"A narrow-waveband spectral index that tracks diurnal changes in photosynthetic efficiency.","volume":"41","author":"Gamon","year":"1992","journal-title":"Remote Sensing of Environment"},{"key":"2025102218195477900_R14","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.rse.2010.08.023","article-title":"The photochemical reflectance index (PRI) and the remote sensing of leaf, canopy and ecosystem radiation use efficiencies. 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