{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:53:55Z","timestamp":1761710035792,"version":"build-2065373602"},"reference-count":59,"publisher":"CSIRO Publishing","issue":"6","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":6166,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,9]]},"abstract":"<jats:p>Nitric oxide (NO) plays an important role in prostaglandin secretion and angiogenesis in the reproductive system. In the present study, the roles of the NO donor spermine NONOate and tumour necrosis factor-a (TNF; as a positive control) in prostaglandin production and angiogenic activity of equine endometria during the oestrous cycle were evaluated. In addition, the correlation between NO production and the expression of key prostaglandin synthase proteins was determined. The protein expression of prostaglandin F synthase (PGFS) increased in early and mid-luteal stages, whereas that of prostaglandin E synthase (PGES) was increased in the early luteal stage. The in vitro release of NO was highest after ovulation. There was a high correlation between NO production and PGES expression, as well as NO release and PGFS expression. There were no differences detected in prostaglandin H synthase 2 (PTGS-2) throughout the oestrous cycle and there was no correlation between PTGS-2 expression and NO. In TNF- or spermine-treated endometria, the expression of prostaglandin (PG) E2 increased in the early and mid-luteal phases, whereas that of PGF2a increased in the follicular and late luteal phases. Bovine aortic endothelial cell (BAEC) proliferation was stimulated in TNF-treated follicular-phase endometria. However, in spermine-treated endometria, NO delivered from its donor had no effect, or even an inhibitory effect, on BAEC proliferation. In conclusion, despite no change in PTGS-2 expression throughout the oestrous cycle in equine endometrial tissue, there were changes observed in the expression of PGES and PGFS, as well as in the production of PGE2 and PGF2a. In the mare, NO is involved in the secretory function of the endometrium, modulating PGE2 and PGF2a production. Even though TNF caused an increase in the production of angiogenic factors and prostaglandins, its complex action in mare uterus should be elucidated.<\/jats:p>","DOI":"10.1071\/rd08015","type":"journal-article","created":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T01:39:11Z","timestamp":1215567551000},"page":"674-683","source":"Crossref","is-referenced-by-count":25,"title":["Actions of a nitric oxide donor on prostaglandin production and angiogenic activity in the equine endometrium"],"prefix":"10.1071","volume":"20","author":[{"given":"Ros\u00e1rio P.","family":"Roberto da Costa","sequence":"first","affiliation":[{"name":"ADepartment of Animal Sciences, Escola Superior Agr\u00e1ria de Coimbra, Bencanta, 3040-316 Coimbra, Portugal."}]},{"given":"Ana S.","family":"Costa","sequence":"additional","affiliation":[{"name":"BCentro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Technical University of Lisbon, 1300-477 Lisboa, Portugal."}]},{"given":"Anna J.","family":"Korzekwa","sequence":"additional","affiliation":[{"name":"CInstitute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland."}]},{"given":"Rafal","family":"Platek","sequence":"additional","affiliation":[{"name":"CInstitute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland."}]},{"given":"Marta","family":"Siemieniuch","sequence":"additional","affiliation":[{"name":"CInstitute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland."}]},{"given":"Ant\u00f3nio","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"BCentro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Technical University of Lisbon, 1300-477 Lisboa, Portugal."},{"name":"CInstitute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland."}]},{"given":"Dale A.","family":"Redmer","sequence":"additional","affiliation":[{"name":"DDepartment of Animal and Range Sciences, North Dakota State University, Fargo, ND 58105-5727, USA."}]},{"given":"Jos\u00e9 Robalo","family":"Silva","sequence":"additional","affiliation":[{"name":"BCentro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Technical University of Lisbon, 1300-477 Lisboa, Portugal."}]},{"given":"Dariusz J.","family":"Skarzynski","sequence":"additional","affiliation":[{"name":"CInstitute of Animal Reproduction and Food Research, 10-747 Olsztyn, Poland."}]},{"given":"Gra\u00e7a","family":"Ferreira-Dias","sequence":"additional","affiliation":[{"name":"BCentro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria, Technical University of Lisbon, 1300-477 Lisboa, Portugal."},{"name":"ECorresponding author. Email: gmlfdias@fmv.utl.pt"}]}],"member":"67","published-online":{"date-parts":[[2008,7,9]]},"reference":[{"key":"2025102818403205300_R1"},{"key":"2025102818403205300_R2"},{"key":"2025102818403205300_R3"},{"key":"2025102818403205300_R4"},{"key":"2025102818403205300_R5"},{"key":"2025102818403205300_R6"},{"key":"2025102818403205300_R7"},{"key":"2025102818403205300_R8"},{"key":"2025102818403205300_R9"},{"key":"2025102818403205300_R10"},{"key":"2025102818403205300_R11"},{"key":"2025102818403205300_R12"},{"key":"2025102818403205300_R13"},{"key":"2025102818403205300_R14"},{"key":"2025102818403205300_R15"},{"key":"2025102818403205300_R16"},{"key":"2025102818403205300_R17"},{"key":"2025102818403205300_R18"},{"key":"2025102818403205300_R19"},{"key":"2025102818403205300_R20"},{"key":"2025102818403205300_R21"},{"key":"2025102818403205300_R22"},{"key":"2025102818403205300_R23"},{"key":"2025102818403205300_R24"},{"key":"2025102818403205300_R25"},{"key":"2025102818403205300_R26"},{"key":"2025102818403205300_R27"},{"key":"2025102818403205300_R28"},{"key":"2025102818403205300_R29"},{"key":"2025102818403205300_R30"},{"key":"2025102818403205300_R31"},{"key":"2025102818403205300_R32"},{"key":"2025102818403205300_R33"},{"key":"2025102818403205300_R34"},{"key":"2025102818403205300_R35"},{"key":"2025102818403205300_R36"},{"key":"2025102818403205300_R37"},{"key":"2025102818403205300_R38"},{"key":"2025102818403205300_R39"},{"key":"2025102818403205300_R40"},{"key":"2025102818403205300_R41"},{"key":"2025102818403205300_R42"},{"key":"2025102818403205300_R43"},{"key":"2025102818403205300_R44"},{"key":"2025102818403205300_R45"},{"key":"2025102818403205300_R46"},{"key":"2025102818403205300_R47"},{"key":"2025102818403205300_R48"},{"key":"2025102818403205300_R49"},{"key":"2025102818403205300_R50"},{"key":"2025102818403205300_R51"},{"key":"2025102818403205300_R52"},{"key":"2025102818403205300_R53"},{"key":"2025102818403205300_R54"},{"key":"2025102818403205300_R55"},{"key":"2025102818403205300_R56"},{"key":"2025102818403205300_R57"},{"key":"2025102818403205300_R58"},{"key":"2025102818403205300_R59"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/20\/6\/674\/301991\/rd08015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/20\/6\/674\/301991\/rd08015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:40:36Z","timestamp":1761691236000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/20\/6\/674\/48590\/Actions-of-a-nitric-oxide-donor-on-prostaglandin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,9]]},"references-count":59,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,7,9]]}},"URL":"https:\/\/doi.org\/10.1071\/rd08015","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"type":"print","value":"1031-3613"},{"type":"electronic","value":"1448-5990"}],"subject":[],"published":{"date-parts":[[2008,7,9]]}}}