{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T22:27:30Z","timestamp":1776378450526,"version":"3.51.2"},"reference-count":38,"publisher":"CSIRO Publishing","issue":"5","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":4374,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,19]]},"abstract":"<jats:p>Interspecific testicular germ cell (TGC) transplantation was investigated in two commercial flatfish species. Testes from donor species (Senegalese sole) were evaluated using classical histological techniques (haematoxylin\u2013eosin staining and haematoxylin\u2013light green\u2013orange G\u2013acid fuchsine staining), in situ hybridisation and immunohistochemical analysis. Both Ssvasa1\u20132 mRNAs and SsVasa protein allowed the characterisation of TGCs, confirming the usefulness of the vasa gene in the detection of Senegalese sole TGCs. Xenogenic transplants were carried out using TGCs from one-year-old Senegalese sole into turbot larvae. Propidium iodide\u2013SYBR-14 and 4',6'-diamidino-2-phenylindole (DAPI) staining showed that 87.98% of the extracted testicular cells were viable for microinjection and that 15.63% of the total recovered cells were spermatogonia. The vasa gene was characterised in turbot recipients using cDNA cloning. Smvasa mRNA was confirmed as a germ cell-specific molecular marker in this species. Smvasa expression analysis during turbot ontogeny was carried out before Senegalese sole TGC transplants into turbot larvae. Turbot larvae at 18 days after hatching (DAH) proved to be susceptible to manipulation procedures. High survival rates (83.75 \u00b1 15.90 \u2013 100%) were obtained for turbot larvae at 27, 34 and 42 DAH. These data highlight the huge potential of this species for transplantation studies. Quantitative PCR was employed to detect Senegalese sole vasa mRNAs (Ssvasa1\u20132) in the recipient turbot larvae. The Ssvasa mRNAs showed a significant increase in relative expression in 42-DAH microinjected larvae three weeks after treatment, showing the proliferation of Senegalese sole spermatogonia in transplanted turbot larvae.<\/jats:p>","DOI":"10.1071\/rd13103","type":"journal-article","created":{"date-parts":[[2013,6,4]],"date-time":"2013-06-04T20:20:46Z","timestamp":1370377246000},"page":"690-702","source":"Crossref","is-referenced-by-count":21,"title":["Development of interspecies testicular germ-cell transplantation in flatfish"],"prefix":"10.1071","volume":"26","author":[{"given":"Tiziana","family":"Pacchiarini","sequence":"first","affiliation":[{"name":"AInstitute of Marine Science of Andalusia- ICMAN.CSIC, Av Republica Saharaui 2, 11510 Puerto Real, C\u00e1diz, Spain."}]},{"given":"Carmen","family":"Sarasquete","sequence":"additional","affiliation":[{"name":"AInstitute of Marine Science of Andalusia- ICMAN.CSIC, Av Republica Saharaui 2, 11510 Puerto Real, C\u00e1diz, Spain."}]},{"given":"Elsa","family":"Cabrita","sequence":"additional","affiliation":[{"name":"AInstitute of Marine Science of Andalusia- ICMAN.CSIC, Av Republica Saharaui 2, 11510 Puerto Real, C\u00e1diz, Spain."},{"name":"BCorresponding author. Email: elsa.cabrita@icman.csic.es"}]}],"member":"67","published-online":{"date-parts":[[2013,6,5]]},"reference":[{"key":"2025102819440851600_R1","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1046\/J.1444-2906.2000.00047.X","article-title":"A method for estimating the number of mitotic divisions in fish testis.","volume":"66","author":"Ando","year":"2000","journal-title":"Fish. Sci."},{"key":"2025102819440851600_R2","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ygcen.2010.10.007","article-title":"Cloning and sequence analysis of a vasa homolog in the European sea bass (Dicentrarchus labrax): Tissue distribution and mRNA expression levels during early development and sex differentiation.","volume":"170","author":"Bl\u00e1zquez","year":"2011","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102819440851600_R3","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1016\/J.AQUACULTURE.2006.08.020","article-title":"Characterization of Senegalese sole, Solea senegalensis, male broodstock in terms of sperm production and quality.","volume":"261","author":"Cabrita","year":"2006","journal-title":"Aquaculture"},{"key":"2025102819440851600_R4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/J.THERIOGENOLOGY.2010.07.003","article-title":"Endocrine and milt response of Senegalese sole, Solea senegalensis, males maintained in captivity.","volume":"75","author":"Cabrita","year":"2011","journal-title":"Theriogenology"},{"key":"2025102819440851600_R5","doi-asserted-by":"publisher","first-page":"12 258","DOI":"10.1073\/PNAS.91.25.12258","article-title":"Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ-cell lineage.","volume":"91","author":"Fujiwara","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025102819440851600_R6","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/J.CBPA.2006.11.014","article-title":"Ovarian development and plasma sex-steroid levels in cultured female Senegalese sole Solea senegalensis.","volume":"146","author":"Garc\u00eda-L\u00f3pez","year":"2007","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"issue":"2","key":"2025102819440851600_R7","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/J.CBPA.2010.11.016","article-title":"Effects of in vivo treatment with dopamine antagonist pimozide and gonadotrophin-releasing hormone agonist (GnRHa) on the reproductive axis of Senegalese sole, Solea senegalensis.","volume":"158","author":"Guzm\u00e1n","year":"2011","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2025102819440851600_R8","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1007\/S12562-010-0314-7","article-title":"Colonization, proliferation and survival of intraperitoneally transplanted yellowtail Seriola quinqueradiata spermatogonia in nibe croaker Nibea mitsukurii recipient.","volume":"77","author":"Higuchi","year":"2011","journal-title":"Fish. Sci."},{"key":"2025102819440851600_R9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1017\/S0025315400040698","article-title":"Studies on egg development and larval rearing of turbot, Scophthalmus maximus.","volume":"52","author":"Jones","year":"1972","journal-title":"J. Mar. Biol. Assoc. UK"},{"issue":"5","key":"2025102819440851600_R10","doi-asserted-by":"crossref","first-page":"700","DOI":"10.2331\/fishsci.65.700","article-title":"Growth and morphological development of laboratory-reared yellowfin tuna Thunnus albacares larvae and early juveniles, with special emphasis on the digestive system.","volume":"65","author":"Kaji","year":"1999","journal-title":"Fish. Sci."},{"key":"2025102819440851600_R11","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/S0925-4773(01)00613-X","article-title":"Two isoforms of vasa homologs in a teleost fish: their differential expression during germ-cell differentiation.","volume":"111","author":"Kobayashi","year":"2002","journal-title":"Mech. Dev."},{"key":"2025102819440851600_R12","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ydbio.2004.04.004","article-title":"Sexual dimorphic expression pattern of a splice variant of zebrafish vasa during gonadal development.","volume":"271","author":"Kr\u00f8vel","year":"2004","journal-title":"Dev. Biol."},{"key":"2025102819440851600_R13","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1002\/MRD.22120","article-title":"Germ-line specific expression of a vasa homologue gene in turbot (Scophthalmus maximus): evidence for vasa localization at cleavage furrows in euteleostei.","volume":"79","author":"Lin","year":"2012","journal-title":"Mol. Reprod. Dev."},{"key":"2025102819440851600_R14","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1038\/337121A0","article-title":"Birth of the DEAD box.","volume":"337","author":"Linder","year":"1989","journal-title":"Nature"},{"key":"2025102819440851600_R15","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/METH.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2013(??Ct).","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2025102819440851600_R16"},{"key":"2025102819440851600_R17","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1095\/BIOLREPROD.111.097873","article-title":"Production of donor-derived offspring by allogeneic transplantation of spermatogonia in the yellowtail (Seriola quinqueradiata).","volume":"86","author":"Morita","year":"2012","journal-title":"Biol. Reprod."},{"key":"2025102819440851600_R18","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1007\/S12562-008-0021-9","article-title":"cDNA cloning and expression analysis of a vasa-like gene in Pacific bluefin tuna Thunnus orientalis.","volume":"75","author":"Nagasawa","year":"2009","journal-title":"Fish. Sci."},{"key":"2025102819440851600_R19","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/S10695-008-9252-Z","article-title":"An overview of functional and stereological evaluation of spermatogenesis and germ-cell transplantation in fish.","volume":"35","author":"N\u00f3brega","year":"2009","journal-title":"Fish Physiol. Biochem."},{"key":"2025102819440851600_R20","doi-asserted-by":"publisher","first-page":"2725","DOI":"10.1073\/PNAS.0509218103","article-title":"Testicular germ cells can colonize sexually undifferentiated embryonic gonads and produce functional eggs in fish.","volume":"103","author":"Okutsu","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025102819440851600_R21","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1126\/SCIENCE.1145626","article-title":"Production of trout offspring from triploid salmon parents.","volume":"317","author":"Okutsu","year":"2007","journal-title":"Science"},{"key":"2025102819440851600_R22","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/J.CRYOBIOL.2012.07.017","article-title":"Transplantation of cryopreserved testicular germ cells in flatfish species: a useful technique for reproductive biotechnology.","volume":"65","author":"Pacchiarini","year":"2012","journal-title":"Cryobiology"},{"key":"2025102819440851600_R23","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1071\/RD11240","article-title":"Solea senegalensis vasa transcripts: molecular characterisation, tissue distribution and developmental expression profiles.","volume":"25","author":"Pacchiarini","year":"2013","journal-title":"Reprod. Fertil. Dev."},{"key":"2025102819440851600_R24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0044-8486(96)01326-9","article-title":"Ontogeny of the lymphoid organs in the turbot Scophtalmus maximus: a light and electron microscope study.","volume":"144","author":"Padr\u00f3s","year":"1996","journal-title":"Aquaculture"},{"key":"2025102819440851600_R25","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1002\/j.1460-2075.1992.tb05330.x","article-title":"Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A.","volume":"11","author":"Pause","year":"1992","journal-title":"EMBO J."},{"key":"2025102819440851600_R26","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.cbpa.2010.04.017","article-title":"Cloning and differential expression pattern of vasa in the developing and recrudescing gonads of catfish, Clarias gariepinus.","volume":"157","author":"Raghuveer","year":"2010","journal-title":"Comp. Biochem. Physiol. A"},{"key":"2025102819440851600_R27"},{"issue":"2","key":"2025102819440851600_R28","first-page":"211","article-title":"New tetrachromic VOF stain (type III-g.s) for normal and pathological fish tissues.","volume":"49","author":"Sarasquete","year":"2005","journal-title":"Eur. J. Histochem."},{"key":"2025102819440851600_R29","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/J.YGCEN.2009.02.013","article-title":"Spermatogenesis in fish.","volume":"165","author":"Schulz","year":"2010","journal-title":"Gen. Comp. Endocrinol."},{"key":"2025102819440851600_R30","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1006\/DBIO.1998.9130","article-title":"Expression of vasa (vas)-related genes in germline cells and totipotent somatic stem cells of planarians.","volume":"206","author":"Shibata","year":"1999","journal-title":"Dev. Biol."},{"key":"2025102819440851600_R31","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1095\/BIOLREPROD.103.017624","article-title":"Generation of live fry from intraperitoneally transplanted primordial germ cells in rainbow trout.","volume":"69","author":"Takeuchi","year":"2003","journal-title":"Biol. Reprod."},{"key":"2025102819440851600_R32","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1038\/430629A","article-title":"Biotechnology: surrogate broodstock produces salmonids.","volume":"430","author":"Takeuchi","year":"2004","journal-title":"Nature"},{"key":"2025102819440851600_R33","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1095\/BIOLREPROD.109.077701","article-title":"Establishment of spermatogonial cell transplantation in Nibe croaker (Nibea mitsukurii).","volume":"81","author":"Takeuchi","year":"2009","journal-title":"Biol. Reprod."},{"key":"2025102819440851600_R34","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1002\/DVDY.20410","article-title":"Differential expression of vasa RNA and protein during spermatogenesis and oogenesis in the gibel carp (Carassius auratus gibelio), a bisexually and gynogenetically reproducing vertebrate.","volume":"233","author":"Xu","year":"2005","journal-title":"Dev. Dyn."},{"key":"2025102819440851600_R35","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1095\/BIOLREPROD.107.064667","article-title":"Flow-cytometric isolation of testicular germ cells from rainbow trout (Oncorhynchus mykiss) carrying the green fluorescent protein gene driven by trout vasa regulatory regions.","volume":"78","author":"Yano","year":"2008","journal-title":"Biol. Reprod."},{"key":"2025102819440851600_R36","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1095\/BIOLREPROD.109.081281","article-title":"Chub mackerel gonads support colonization, survival and proliferation of intraperitoneally transplanted xenogenic germ cells.","volume":"82","author":"Yazawa","year":"2010","journal-title":"Biol. Reprod."},{"key":"2025102819440851600_R37","doi-asserted-by":"crossref","first-page":"3157","DOI":"10.1242\/dev.124.16.3157","article-title":"Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells.","volume":"124","author":"Yoon","year":"1997","journal-title":"Development"},{"key":"2025102819440851600_R38","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/J.CBD.2010.05.003","article-title":"Spermatogonial transplantation in fish: a novel method for the preservation of genetic resources.","volume":"6","author":"Yoshizaki","year":"2011","journal-title":"Comp. Biochem. Physiol. D Genomics Proteomics"}],"container-title":["Reproduction, Fertility and Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/26\/5\/690\/311271\/rd13103.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/connectsci.au\/rd\/article-pdf\/26\/5\/690\/311271\/rd13103.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:44:14Z","timestamp":1761695054000},"score":1,"resource":{"primary":{"URL":"https:\/\/connectsci.au\/rd\/article\/26\/5\/690\/50059\/Development-of-interspecies-testicular-germ-cell"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,5]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,6,19]]}},"URL":"https:\/\/doi.org\/10.1071\/rd13103","relation":{},"ISSN":["1031-3613","1448-5990"],"issn-type":[{"value":"1031-3613","type":"print"},{"value":"1448-5990","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,5]]}}}