{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:38:32Z","timestamp":1777448312379,"version":"3.51.4"},"reference-count":37,"publisher":"CSIRO Publishing","issue":"7","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3793,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,18]]},"abstract":"<jats:p>Phosphoprotein phosphatase 1 (PPP1) catalytic subunit gamma 2 (PPP1CC2), a PPP1 isoform, is largely restricted to testicular germ cells and spermatozoa. The key to understanding PPP1 regulation in male germ cells lies in the identification and characterisation of its interacting partners. This study was undertaken to determine the expression patterns of the several ankyrin repeat protein variant 2 (SARP2), a PPP1-interacting protein, in testis and spermatozoa. SARP2 was found to be highly expressed in testis and spermatozoa, and its interaction with human spermatozoa endogenous PPP1CC2 was confirmed by immunoprecipitation. Expression analysis by RT-qPCR revealed that SARP2 and PPP1CC2 mRNA levels were significantly higher in the spermatocyte fraction. However, microscopy revealed that SARP2 protein was only present in the nucleus of elongating and mature spermatids and in spermatozoa. In spermatozoa, SARP2 was prominently expressed in the connecting piece and flagellum, as well as, to a lesser extent, in the acrosome. A yeast two-hybrid approach was used to detect SARP2-interacting proteins and a relevant interaction with a novel sperm-associated antigen 9 (SPAG9) variant, a testis and spermatozoa-specific c-Jun N-terminal kinase-binding protein, was validated in human spermatozoa. Given the expression pattern of SARP2 and its association with PPP1CC2 and SPAG9, it may play a role in spermiogenesis and sperm function, namely in sperm motility and the acrosome reaction.<\/jats:p>","DOI":"10.1071\/rd14303","type":"journal-article","created":{"date-parts":[[2015,1,6]],"date-time":"2015-01-06T19:54:44Z","timestamp":1420574084000},"page":"1009-1019","source":"Crossref","is-referenced-by-count":2,"title":["Characterisation of several ankyrin repeat protein variant 2, a phosphoprotein phosphatase 1-interacting protein, in testis and spermatozoa"],"prefix":"10.1071","volume":"28","author":[{"given":"Joana Vieira","family":"Silva","sequence":"first","affiliation":[{"name":"ALaboratory of Signal Transduction, Biology Department and Institute for Research in Biomedicine \u2013 iBiMED, Health Sciences Program, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193, Aveiro, Portugal."}]},{"given":"Lu\u00eds","family":"Korrodi-Greg\u00f3rio","sequence":"additional","affiliation":[{"name":"ALaboratory of Signal Transduction, Biology Department and Institute for Research in Biomedicine \u2013 iBiMED, Health Sciences Program, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193, Aveiro, Portugal."}]},{"given":"Georg","family":"Luers","sequence":"additional","affiliation":[{"name":"BInstitute for Anatomy and Experimental Morphology, Center for Experimental Medicine, University Hamburg-Eppendorf, D-20246 Hamburg, Germany."}]},{"given":"Maria Jo\u00e3o","family":"Cardoso","sequence":"additional","affiliation":[{"name":"ALaboratory of Signal Transduction, Biology Department and Institute for Research in Biomedicine \u2013 iBiMED, Health Sciences Program, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193, Aveiro, Portugal."}]},{"given":"Ant\u00f3nio","family":"Patr\u00edcio","sequence":"additional","affiliation":[{"name":"CInfante D. Pedro CHBV Hospital, 3810-193 Aveiro, Portugal."}]},{"given":"Nuno","family":"Maia","sequence":"additional","affiliation":[{"name":"CInfante D. Pedro CHBV Hospital, 3810-193 Aveiro, Portugal."}]},{"given":"Edgar F.","family":"da Cruz e Silva","sequence":"additional","affiliation":[{"name":"ALaboratory of Signal Transduction, Biology Department and Institute for Research in Biomedicine \u2013 iBiMED, Health Sciences Program, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193, Aveiro, Portugal."}]},{"given":"Margarida","family":"Fardilha","sequence":"additional","affiliation":[{"name":"ALaboratory of Signal Transduction, Biology Department and Institute for Research in Biomedicine \u2013 iBiMED, Health Sciences Program, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193, Aveiro, Portugal."},{"name":"DCorresponding author. 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