{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T23:48:40Z","timestamp":1773704920221,"version":"3.50.1"},"reference-count":45,"publisher":"CSIRO Publishing","issue":"12","license":[{"start":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T00:00:00Z","timestamp":1748304000000},"content-version":"vor","delay-in-days":3624,"URL":"https:\/\/doi.org\/10.1071\/journalslicense"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,10,11]]},"abstract":"<jats:p>The basic pathway of oocyte development and its regulation is evolutionarily conserved among vertebrates; however, little is known about the role of hormones at the first stages (Stages I and II) of follicle development in fish. In the present study, zebrafish follicles at Stages I and II were exposed in vitro to the reproductive hormones 17\u00df-oestradiol (E2), 11-ketotestosterone (11KT), 17,20\u00df-dihydroxy-4-pregnen-3-one (DHP) and to the secondary messenger dibutyryl cyclic adenosine monophosphate (db-cAMP) at a concentration of 1 \u00b5M for a 48-h period. Morphological alterations of the ooplasm were assessed by transmission electron microscopy and of the granulosa cell layer by quantitative stereology. Expression of mRNA was analysed for cell-cycle genes (cyclin B and E) and resident proteins of the endoplasmic reticulum (calnexin and 78-kDa glucose-regulated protein (grp78\/bip)). E2 and db-cAMP stimulated the presence of endoplasmic reticulum in the ooplasm and calnexin mRNA increased in the db-cAMP treatment, but also in response to 11KT and DHP. 11KT, DHP and db-cAMP inhibited the progression of the cell cycle in the granulosa\u2013theca cell layer, indicated by a reduction of the nucleus volume-weighted size of granulosa cells and of increased cyclin E mRNA expression. Reproductive hormones had different effects on the ooplasm and the granulosa\u2013theca cell layer of zebrafish follicles, predominantly at Stage II.<\/jats:p>","DOI":"10.1071\/rd15100","type":"journal-article","created":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T01:28:56Z","timestamp":1435195736000},"page":"1945-1952","source":"Crossref","is-referenced-by-count":3,"title":["Reproductive hormones affect follicular cells and ooplasm of Stage I and II oocytes in zebrafish"],"prefix":"10.1071","volume":"28","author":[{"given":"Maria L\u00edgia","family":"Sousa","sequence":"first","affiliation":[{"name":"ACIIMAR \u2013 Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto \u2013 University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal."},{"name":"BICBAS \u2013 Institute of Biomedical Sciences Abel Salazar, UPorto \u2013 University of Porto, Laboratory of Histology and Embryology, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal."}]},{"given":"Ana","family":"Silva","sequence":"additional","affiliation":[{"name":"BICBAS \u2013 Institute of Biomedical Sciences Abel Salazar, UPorto \u2013 University of Porto, Laboratory of Histology and Embryology, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal."}]},{"given":"Fernanda","family":"Malh\u00e3o","sequence":"additional","affiliation":[{"name":"ACIIMAR \u2013 Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto \u2013 University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal."},{"name":"BICBAS \u2013 Institute of Biomedical Sciences Abel Salazar, UPorto \u2013 University of Porto, Laboratory of Histology and Embryology, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal."}]},{"given":"Maria Jo\u00e3o","family":"Rocha","sequence":"additional","affiliation":[{"name":"ACIIMAR \u2013 Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto \u2013 University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal."},{"name":"BICBAS \u2013 Institute of Biomedical Sciences Abel Salazar, UPorto \u2013 University of Porto, Laboratory of Histology and Embryology, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal."},{"name":"CISCS-N \u2013 Superior Institute of Health Sciences-North, CESPU, Cooperative Higher Education, Polytechnic and University, Rua Central de Gandra 1317, 4585-116 Gandra, Paredes, Portugal."}]},{"given":"Eduardo","family":"Rocha","sequence":"additional","affiliation":[{"name":"ACIIMAR \u2013 Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto \u2013 University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal."},{"name":"BICBAS \u2013 Institute of Biomedical Sciences Abel Salazar, UPorto \u2013 University of Porto, Laboratory of Histology and Embryology, R. Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal."}]},{"given":"Ralph","family":"Urbatzka","sequence":"additional","affiliation":[{"name":"ACIIMAR \u2013 Interdisciplinary Centre of Marine and Environmental Research, CIMAR Associated Laboratory, UPorto \u2013 University of Porto, Laboratory of Cellular, Molecular and Analytical Studies, Rua dos Bragas 289, 4050-123 Porto, Portugal."},{"name":"DCorresponding author. Email: rurbatzka@ciimar.up.pt"}]}],"member":"67","published-online":{"date-parts":[[2015,6,25]]},"reference":[{"key":"2025102820194911400_R1","doi-asserted-by":"publisher","first-page":"2938","DOI":"10.1093\/HUMREP\/17.11.2938","article-title":"Calcium-binding proteins and calcium-release channels in human maturing oocytes, pronuclear zygotes and early preimplantation embryos.","volume":"17","author":"Balakier","year":"2002","journal-title":"Hum. 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